Monolith NT.Automated Microscale Thermophoresis (MST)

NanoTemper Technologies

  • Determination of molecular interaction kinetics (Kd) and target-ligand library screening using microscale thermophoresis (MST) technology
  • Uses chips containing 24 micro-capillaries
    • Sample volume of 15 µL /capillary for loading
    • Capacity and throughput of instrument is up to four capillary chips (96 samples) per experiment analyzed in less than 30 minutes
  • Two detection modes available
    • PicoRED fluorescent detector channel detector
      • Provides Kd values from pM up to the mM range for protein-protein and protein-ligand interactions
      • Various PicoRed protein-labelling strategies including linkage through poly-histidine tags, amine groups and cysteine residues
    • LabelFree tryptophan fluorescence detector channel
      • No additional protein labelling required
      • Provides Kd values from nM up to the mM range for protein-ligand interactions