The Amino Acid Analysis lab has served over 250 academic and industry researchers worldwide since its inception in 1984, providing access to high quality proteomics applications at a competitive price. We routinely perform accurate analyses of sample concentration, composition, and purity using 500 picomoles (and as little as 50 picomoles) of purified protein by HPLC. This methodology is especially useful for protein or peptide samples that require hydrolysis (breakdown into amino acids). Services include:
For Purified Protein/Peptide Samples After Hydrolysis:
- Estimate amount of protein and degree of purity
- Estimate concentration of protein/peptide solution
- Determine amino acid composition, estimate amount of unusual amino acids
- Determine the complete amino acid profile of foodstuffs
- Estimate tryptophan and/or cysteine content of a peptide
For Amino Acid Samples Without Hydrolysis:
- Determine and estimate specific free amino acids in media and solutions
- Determine physiological amino acids in biological fluids
Note: quantification of free amino acids can also be accomplished by our Small Molecule Mass Spectrometry services.
For more information about amino acid analysis, please contact Leanne Wybenga-Groot (leanne.wybenga-groot@sickkids.ca)
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