Resources
Fluorescence Spectra Viewers
- BD Spectrum Viewer
- BioLegend Spectra Analyzer
- FluoroFinder Spectra Viewer
- Life Technologies SpectraViewer
- ThermoFisher FluorPlan Spectra Viewer
Fluorochrome Tables
- BD Relative Fluorochrome Brightness
- BioLegend Fluorochrome Brightness Index
- ThermoFisher Fluorophore Charts
- BD Multicolour Fluorochrome/Laser Chart
- BD Fluorochrome Specifications Chart
Fluorochrome Panel Design Tools
- BioLegend panel selector
- BioLegend Instrument Guide/Fluorophore Selector
- BD FACSelect Buffer Compatibility Resource
- FluoroFinder Panel Design Tool
Fluorochrome/Dye Info
User guides
- BD FACS Diva Software User Guide
- BD Biosciences Instrument User Guides
- AutoMACS Pro Separator – short instructions
Tech notes
- Tips on Cell Preparation for Flow Cytometric Analysis and Sorting
- Selecting Reagents for Multicolor Flow Cytometry
- Establishing Optimum Baseline PMT Gains to Maximize Resolution
- Analyzing Protein Phosphorylation Pathways in Heterogeneous Samples (BD Webinar slides)
- Optimizing Intracellular Flow Cytometry (BD Webinar slides)
Flow Cytometry
- “Practical Flow Cytometry,” e- book by Howard Shapiro
- Seventeen-colour flow cytometry: unravelling the immune system
- Interpreting flow cytometry data: a guide for the perplexed
- A practical approach to multicolor flow cytometry for immunophenotyping
- Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
- A new “Logicle” display method avoids deceptive effects of logarithmic scaling for low signals and compensated data
- Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry
- Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats
Instruments
Antibodies and Reagents
- AbCam
- BD Biosciences
- Beckman Coulter
- Biolegend
- Cell Signaling Technology
- eBioscience
- Jackson Immunoresearch
- Life Technologies
- Novus Biologicals
Flow Cytometry Particles
Assisted Acquisition Sample Info Form: Sample_Acquisition_Form-3