Antibody Conjugations for Your Panels
Metal-tagged Abs specific for markers commonly used in mass cytometry are commercially available but are expensive since there is only a single vendor. To reduce costs and allow greater flexibility in experimental design, we perform custom metal conjugations of purified antibodies that you purchase or produce from hybridomas.
This service provides the most cost-effective way to build panels optimized for your specific experimental context. Add Abs (ideally validated to work in flow cytometry or on fixed tissue sections as per the protocol you will use) to our base immune profiling panels for mouse or human or start from scratch and design your own. You will need to provide us with 100-150 micrograms of purified, protein carrier free Ab for a single reaction).
Please contact Tina Chen for the list of currently available metal tags and pricing.
Optimizing Your Panels
Though panel design for CyTOF is simpler than for flow cytometry, there are some things to consider as described here.