AFBM Instrumentation
The AFBM is home to multiple mass spectrometers, HPLCs, and other instuments for small molecule and biomarker analysis.
Clicking on the instrument photo will take you to the specific service page to learn more.
Liquid Chromatography Mass Spectrometry (LC-MS/MS)
Sciex QTrap 5500 with SelexION + Agilent 1200 HPLC
- Mass range : 40 to 1250 m/z
- ESI and APCI Ionization
- Multiple scan modes: MS, MS/MS, Product Ion, Precursor Ion, Neutral Loss, Targeted MR
SelexION
- Differential Ion Mobility Spectrometry
- Added mode of selectivity complimentary to mass spectrometry
- Fast separation of isobaric species
Agilent Binary Analytical flow HPLC System
- Agilent 1200 for analytical flow HPLC separations up to 400 bar
Sciex 6500+ System + Exion HPLC
- Acquired in 2019 (more sensitive than the Sciex 5500)
- Mass range : 40 to 1250 m/z
- ESI and APCI Ionization
- Multiple scan modes: MS, MS/MS, Product Ion, Precursor Ion, Neutral Loss, Targeted MR
Shimadzu Exion LC-20AD Pump System
- Exion for analytical flow HPLC separations up to 1000 bar
Sciex QTrap 5500 + Agilent 1290 uHPLC
- Mass range : 40 to 1250 m/z
- ESI and APCI Ionization
- Multiple scan modes: MS, MS/MS, Product Ion, Precursor Ion, Neutral Loss, Targeted MR
Agilent Binary Analytical flow HPLC System
- Infinity uHPLC 1290 for analytical flow ultra-high pressure applications up to 1200 bar
Sciex 6600 Triple TOF + Agilent 1260 HPLC
- Mass range : 30 – 2250 m/z
- ESI and APCI Ionization
- High Resolution: 30,000 TOF MS and 20,000 MS/MS
- Key instrument for untargeted lipidomics
Agilent Binary Analytical flow HPLC System
- Agilent 1260 for analytical flows enhanced up to 600 bar
Gas Chromatography (GC)
Agilent 7890A GC
- 5975C MSD with EI/CI source
- Flame Ionization Detector (FID)
- Thermal desorption unit for volatiles analysis
High Pressure Liquid Chromatography (HPLC)
Dionex Ultimate 3000 HPLC
- x2 Dual-gradient micro pump system (normal and reverse phase)
- Temperature controlled autosampler and column compartment
- Photodiode array (PDA) detector (190-800 nm, 1 nm spectral resolution)
- Fluorescence detector (200-650 nm)
Agilent 1100 HPLC with ESA Coulochem III Electrochemical detector
- Binary pump
- Temperature controlled autosampler and column compartment
- ESA Coulochem III electrochemical detector: multi-electrode capability permits electrochemical detection from 2 analytical channels in combination with a Guard or conditioning cell for optimum sensitivity and selectivity.
Isotope Ratio Mass Spectrometry (IRMS)
Delta V Plus Isotope Ratio Mass Spectrometer
Flash 2000 Elemental Analyzer with Zero Blank Autosampler
GC IsoLink II
GasBench II
Immunoassays
Luminex 200
For Multiplexing Immunoassays
Up to 100 analytes/per well
Flow Cytometry meets ELISA
Luminex MagPix
Multiplexing Immunoassays
Measure up to 50 analytes/well
CCD camera captures signal
Magnetic Beads
Molecular Devices SpectraMax M2
- Fluorescene
- Absorbance
- Luminescence
- For enzymatic and colourimetric assays and ELISAs