Cell-type deconvolution analyses of tumour-cell-specific clusters from human PFA and C-PA scRNA-seq
UMAP representation of the male embryonic mouse cerebellum scRNA-seq dataset, arrows show the predicted developmental trajectory (Velocyto)
Immunofluorescence of brain tumour markers in PFA cells demonstrate differentiation upon growth factor withdrawal.
Increased senescence as seen by XGal staining of PFA cells cultured in 21% compared to 1% oxygen.
Hypoxia is associated with decreased H3K27 trimethylation in biopsies of PFA tumour tissue.
In vivo optical imaging to visualize medulloblastoma metastasis (DaCosta/Taylor Lab Collaboration)
Developmental trajectory of GABAergic Interneurons
UMAP representation of the female embryonic mouse cerebellum scRNA-seq data set, arrows show the predicted developmental trajectory (Velocyto)
Gliogenic progenitor pathways (E16, E18, P0)
Graphical depiction of childhood cerebellar tumours mirroring conserved fetal transcriptional programs
Medulloblastoma primary tumor in the cerebellum of a Sleeping Beauty Patched1 Het mouse model
Confocal image of spinal meninges with Math1-GFP metastatic tumour cells (green) associating with a blood vessel network (blue and red) in a transgenic medulloblastoma model model.
Confocal image of whole-mount spinal meninges labelled for Math1-GFP metastatic tumour cells (green) and meningeal blood vessels (red) in a transgenic medulloblastoma mouse model.
In vivo live imaging of GFP+ medulloblastoma cells (at 20X) in the leptomeningeal space next to the spinal vein (TRITC+) via a surgically implanted spinal window
Spinal cord of a rat that was injected with Med8A GFP+ medulloblastoma cells in the lateral ventricle.
Med8A GFP+ medulloblastoma cells that attached and survived on the cerebellum after injection into the right lateral ventricle of a rat brain. Notice that a lot of the GFP+ cells are in contact/close to blood vessels.
Staining for Sirpα (green) for neurons and DNA (green) of normal cerebellar and medulloblastoma tissue by imaging mass cytometry
Confocal image of spinal cord cross-section labelled for nuclei (blue), neurons (cyan) and the leptomeninges (magenta). Math1-GFP metastatic tumour cells (green) reside on the surface of the spinal cord within the leptomeningeal space of the transgenic medulloblastoma mouse model.
Confocal image of spinal meninges with Math1-GFP metastatic tumour cells (green) surrounding meningeal blood vessels (red) in a transgenic medulloblastoma mouse model.
Immunofluorescence staining for F4/80+ macrophages and DAPI around GFP+ metastasis on spinal meninges of a Sleeping Beauty Patched1 Het mouse (zoomed regions)
Patient-derived pediatric PFA ependymoma cell lines grown under hypoxic conditions display distinct morphologies (10X).
Patient-derived paediatric PFA ependymoma cell lines grown under hypoxic conditions display distinct morphologies (10X).
Patient-derived paediatric PFA ependymoma cell lines grown under hypoxic conditions display distinct morphologies (10X).
Table depicting Molecular subgroups of Ependymoma
Dimension reduction in tumor and immune cells
BrainTumour Research Centre (BTRC) Christmas party 2019
Going away party for the lab volunteer staff (Michelle Ly, and Vernon (Vernon has now joined back as a Graduate Student).
Xiaochong Wu and Michael Taylor at his surprise 50th birthday party from the lab
Dr. Michael Taylor stands in front of 50th birthday decorations
Dr. Taylor's 50th birthday celebration
A large group of lab members standing together smiling, surrounded by balloons, cakes and a happy birthday banner
Lab members standing together with birthday cakes
Betty and Florence is from Betty's baby shower/maternity leave celebration
"Girls night out" a dinner that Dr. Taylor hosted for the invited potential Postdoctoral fellow candidate Abhirami Visvanathan to visit his lab. Abhirami was taken out for dinner along with few of the ladies from the lab (Abhirami is now part of the lab).
Jonelle, Winnie, Carolina and Michelle is from the Taylor lab Christmas party at Dukes Refresher. That pink drink is quite popular amongst the ladies in our lab!
Switching roles between Michael and the admin.
Several lab members gathered in the park
Lab members sitting around a table at a restaurant
Several lab members standing together in the lobby of a restaurant
Taylor lab going to the indoor Archery facility
Lab members wearing Archery equipment during a team building event
Lab members gathered together for a photo taken outside during the fall
Science
Cell-type deconvolution analyses of tumour-cell-specific clusters from human PFA and C-PA scRNA-seq
UMAP representation of the male embryonic mouse cerebellum scRNA-seq dataset, arrows show the predicted developmental trajectory (Velocyto)
Immunofluorescence of brain tumour markers in PFA cells demonstrate differentiation upon growth factor withdrawal.
Increased senescence as seen by XGal staining of PFA cells cultured in 21% compared to 1% oxygen.
Hypoxia is associated with decreased H3K27 trimethylation in biopsies of PFA tumour tissue.
In vivo optical imaging to visualize medulloblastoma metastasis (DaCosta/Taylor Lab Collaboration)
Developmental trajectory of GABAergic Interneurons
UMAP representation of the female embryonic mouse cerebellum scRNA-seq data set, arrows show the predicted developmental trajectory (Velocyto)
Gliogenic progenitor pathways (E16, E18, P0)
Graphical depiction of childhood cerebellar tumours mirroring conserved fetal transcriptional programs
Medulloblastoma primary tumor in the cerebellum of a Sleeping Beauty Patched1 Het mouse model
Confocal image of spinal meninges with Math1-GFP metastatic tumour cells (green) associating with a blood vessel network (blue and red) in a transgenic medulloblastoma model model.
Confocal image of whole-mount spinal meninges labelled for Math1-GFP metastatic tumour cells (green) and meningeal blood vessels (red) in a transgenic medulloblastoma mouse model.
In vivo live imaging of GFP+ medulloblastoma cells (at 20X) in the leptomeningeal space next to the spinal vein (TRITC+) via a surgically implanted spinal window
Spinal cord of a rat that was injected with Med8A GFP+ medulloblastoma cells in the lateral ventricle.
Med8A GFP+ medulloblastoma cells that attached and survived on the cerebellum after injection into the right lateral ventricle of a rat brain. Notice that a lot of the GFP+ cells are in contact/close to blood vessels.
Staining for Sirpα (green) for neurons and DNA (green) of normal cerebellar and medulloblastoma tissue by imaging mass cytometry
Confocal image of spinal cord cross-section labelled for nuclei (blue), neurons (cyan) and the leptomeninges (magenta). Math1-GFP metastatic tumour cells (green) reside on the surface of the spinal cord within the leptomeningeal space of the transgenic medulloblastoma mouse model.
Confocal image of spinal meninges with Math1-GFP metastatic tumour cells (green) surrounding meningeal blood vessels (red) in a transgenic medulloblastoma mouse model.
Immunofluorescence staining for F4/80+ macrophages and DAPI around GFP+ metastasis on spinal meninges of a Sleeping Beauty Patched1 Het mouse (zoomed regions)
Patient-derived pediatric PFA ependymoma cell lines grown under hypoxic conditions display distinct morphologies (10X).
Patient-derived paediatric PFA ependymoma cell lines grown under hypoxic conditions display distinct morphologies (10X).
Patient-derived paediatric PFA ependymoma cell lines grown under hypoxic conditions display distinct morphologies (10X).
Table depicting Molecular subgroups of Ependymoma
Dimension reduction in tumor and immune cells
Fun
BrainTumour Research Centre (BTRC) Christmas party 2019
Going away party for the lab volunteer staff (Michelle Ly, and Vernon (Vernon has now joined back as a Graduate Student).
Xiaochong Wu and Michael Taylor at his surprise 50th birthday party from the lab
Dr. Michael Taylor stands in front of 50th birthday decorations
Dr. Taylor's 50th birthday celebration
A large group of lab members standing together smiling, surrounded by balloons, cakes and a happy birthday banner
Lab members standing together with birthday cakes
Betty and Florence is from Betty's baby shower/maternity leave celebration
"Girls night out" a dinner that Dr. Taylor hosted for the invited potential Postdoctoral fellow candidate Abhirami Visvanathan to visit his lab. Abhirami was taken out for dinner along with few of the ladies from the lab (Abhirami is now part of the lab).
Jonelle, Winnie, Carolina and Michelle is from the Taylor lab Christmas party at Dukes Refresher. That pink drink is quite popular amongst the ladies in our lab!
Switching roles between Michael and the admin.
Several lab members gathered in the park
Lab members sitting around a table at a restaurant
Several lab members standing together in the lobby of a restaurant
Taylor lab going to the indoor Archery facility
Lab members wearing Archery equipment during a team building event
Lab members gathered together for a photo taken outside during the fall