SPARC Vector Information

Human ORFeome Clones:

• pDONR223 Map and Sequence

Gateway Entry Vector Information:

• pDONR221 Map, pDONR221 Polylinker and Sequence

Mammalian Gene Collection (MGC) Clones:

• MGC Clone Vector Information
• IRAT, IRAU and IRCM Plate Vector List
• MGC IRAT, IRAU and IRCM Plates

SPARC Destination Vectors Summary:

• SPARC Destination Vectors (in PDF)
• SPARC Destination Vectors (in Excel)

Please note: Unfortunately, SPARC can NOT distribute “Empty” vectors!

High-level E. coli Expression Vectors:

• pDEST14 Native Expression (no tag)
  • pDEST 14 Sequence, pDEST 14 Map, pDEST 14 Polylinker
• N-terminal GST
  • pDEST 15 Sequence, pDEST 15 Map, pDEST 15 Polylinker
• N-terminal 6XHis
  • pDEST 17 Sequence, pDEST 17 Map, pDEST 17 Polylinker
• N-terminal 6XHis-Lumio Tag-TEV recognition site
  • pEXP3-DEST Sequence, pEXP3-DEST Map, pEXP3-DEST Polylinker

• [pDEST 14, 15 17 and pEXP3-DEST  gene expression is controlled by the T7 promoter. Transformation into bacterial strains that express the T7 RNA polymerase are required for protein expression]

Mammalian Constitutive Expression Vectors:

• C-terminal V5 epitope-6XHis
  • pcDNA-DEST40 Sequence, pcDNA-DEST40 Map, pcDNA-DEST40 Polylinker
• C-terminal Cycle 3 GFP
  • pcDNA-DEST47 Sequence, pcDNA-DEST47 Map, pcDNA-DEST47 Polylinker
• N-terminal Cycle 3 GFP
  • pcDNA-DEST53 Sequence, pcDNA-DEST53 Map, pcDNA-DEST53 Polylinker
• N-terminal V5 Epitope-TEV recognition site
  • pcDNA3.1 nV5-DEST Sequence, pcDNA3.1 nV5-DEST Map, pcDNA3.1 nV5-DEST Polylinker
• C-terminal V5 Epitope
  • pcDNA 3.2 V5-DEST Sequence, pcDNA 3.2 V5-DEST Map, pcDNA 3.2-DEST Polylinker
• N-terminal 6XHis
  • pDEST 26 Sequence, pDEST 26 Map, pDEST 26 Polylinker
• N-terminal GST
  • pDEST 27 Sequence, pDEST 27 Map, pDEST 27 Polylinker
• N-terminal HA Epitope
  •  pcDNA3.1/nHA-DEST Map and Polylinker
• N-terminal Myc Epitope
  • pcDNA3.1/nMyc-DEST Map and Polylinker
• N-terminal Flag Epitope
  • pcDNA3.1/nFlag-DEST Map and Polylinker
• N-terminal mOrange Fluorescent Protein Fusion
  • pcDNA3.1-nmOrange
• N-terminal mStrawberry Fluorescent Protein Fusion
  • pcDNA3.1-nmStrawberry
• N-terminal mCherry Fluorescent Protein Fusion
  • pcDNA3.1-nmCherry
• C-terminal Emerald GFP Fluorescent Protein Fusion
  • pcDNA6.2 C-EmGFP-DEST Sequence, pcDNA6.2 C-EmGFP-DEST Map, pcDNA6.2 C-EmGFP-DEST Polylinker
• N-terminal Emerald GFP Fluorescent Protein Fusion
  • pcDNA6.2 N-EmGFP-DEST Sequence, pcDNA6.2 N-EmGFP-DEST Map, pcDNA6.2 N-EmGFP-DEST Polylinker

Mammalian Inducible Expression Vectors:

• Tetracycline Operator with N-terminal 6XHis
  • pT-REx-DEST31 Sequence, pT-REx-DEST31 Map, pT-REx-DEST31 Polylinker

Insect Constitutive Expression Vectors:

• Metallothionein promoter with C-terminal V5 Epitope and 6XHis
  • pMT-DEST48 Sequence, pMT-DEST48 Map, pMT-DEST48 Polylinker

Please note: Unfortunately, SPARC can NOT distribute “Empty” vectors!

Control Expression Western Blots for Various Gateway Expression Vectors:

• Western Blots