SPARC houses both human and mouse pGIPZ lentiviral shRNAmir-GFP libraries developed by GE Dharmacon in collaboration with Dr. Greg Hannon (CSHL) and Dr. Steve Elledge (Harvard), offering access to genome wide RNAi resources. The human library contains >145,000 constructs and the Mouse Library >110,000 constructs. These libraries combine the design advantages of microRNA-adapted shRNA with the pGIPZ vector to create a powerful RNAi trigger capable of producing RNAi in a variety of cell types. TurboGFP and shRNAmir are part of a bicistronic transcript allowing the visual marking of shRNAmir expressing cells. Additionally SPARC can package pGIPZ shRNA lentiviral particles for transduction into difficult cell lines, including primary and non-dividing cells.
Distribution of pGIPZ shRNA is limited to SickKids Investigators
From knockdown of single genes to complete pathways SPARC’s shRNA screening services will accelerate the pace of discovery and augment your research. pGIPZ shRNA constructs are available at significant savings to all SickKids investigators. Please see the pGIPZ shRNA Price List for details.Please contact Christopher Fladd for more details.
To place orders:
We request that you Email Christopher Fladd a list of the genes you are interested in knocking down. We will then provide you with an Excel file with all the available shRNA hairpins available. You can then place an order via QReserve.
If you require any assistance in identifying individual clones or performing searches, please don’t hesitate to contact us.
Available pGIPZ gene families and pathways defined by Gene Ontology:
pGIPZ shRNAmir gene families provide the advantages of shRNAmir design combined with the convenience of Gene ontology (GO) annotated collections targeting all genes in a particular gene family or biological pathway.
The arrayed sets contain shRNAmir from the human and mouse GIPZ lentiviral shRNAmir libraries that target all genes falling under specific GO terms. These collections will greatly simplify pathway analysis, target identification and validation.
| Human pGIZP Gene Families | Mouse pGIPZ Gene Families |
| Angiogenesis | Angiogenesis |
| Apoptosis | Apoptosis |
| Cell Cycle | Cell Cycle |
| GPCR | GPCR |
| Kinase | Kinase |
| Phosophatase | Phosphatase |
| Transcription Factor | Transcription Factor |
| Ubiquitin | Ubiquitin |
| Ubiquitin ligase | Ubiquitin Ligase |
Detailed information and instructions can be found in the pGIPZ Manual, Arrest-In Manual, Lentiviral shRNAmir TechNote