How to place an order:

SickKids investigators are REQUIRED to have an approved biosafety protocol before virus particles can be released.

External customers must provide documentation from their institution demonstrating approval to work with lentivirus before particles can be released.

All Lenti Orders must be place through SPARC Drug Discovery QReserve and include the SPARC Viral Particle Request Form

Please direct any questions about Lentivirus to Chris Fladd cfladd@sickkids.ca &/or Jonathan Sayewich jonathan.sayewich@sickkids.ca

The Plasmid DNA

  1. The DNA should be delivered in a sealed 1.5 mL microtube, clearly labeled as described above, including concentration
  2. The DNA should be purified by maxi/midi columns. (mini-preps are not suitable)
  3. For small scale production 50ug of DNA is required for each T-75 flask (~10mL of viral supernatant) vector prep. Concentration should be 1.0ug/uL
  4. For large scale production 250ug of DNA is required (~50mL of viral supernatant) vector prep. Concentration should be 1.0ug/uL

Picking up Viral Particles

  1. You will get an email message indicating the time to pick up your order
  2. Viral stock(s) will be stored at  -80 C  or 4 C at the request of the customer
  3. If determination of the titre is requested a minimum of five days is required after harvesting supernatant