Selected publications

1996-1999

  1. Staub O, Dho S, Henry P, Correa J, Ishikawa T, McGlade J, ROTIN D. WW domains of Nedd4 bind to the proline-rich PY motifs in the epithelial Na+channel deleted in Liddle’s syndrome. EMBO J. (1996) 15: 2371-80.
  2. Staub O, Gautschi I, Ishikawa T, Breitschopf K, Ciechanover A, Schild L, ROTIN D. Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination. EMBO J.(1997) 16: 6325-36.
  3. Wallace MJ, Batt J, Fladd CA, Henderson JT, Skarnes W, ROTIN D. Neuronal defects and posterior pituitary hypoplasia in mice lacking the receptor tyrosine phosphatase PTPsigma.  Nature Genet. (1999) 21: 334-8.
  4. Abriel H, Loffing J, Rebhun JF, Pratt JH, Schild L, Horisberger JD, ROTIN D, Staub O. Defective regulation of the epithelial Na+channel by Nedd4 in Liddle’s syndrome.  J. Clin. Invest. (1999) 103: 667-73.

2000-2010

  1. Plant PJ, Lafont F, Lecat S, Verkade P, Simons K, ROTIN D. Apical membrane targeting of Nedd4 is mediated by an association of its C2 domain with annexin XIIIb. J. Cell Biol. (2000) 149: 1473-84.
  2. Kanelis V, ROTIN D, Forman-Kay JD. Solution structure of a Nedd4 WW domain-ENaC peptide complex. Nature Struct. Biol. (2001) 8: 407-12.
  3. Pak Y, Glowacka WK, Bruce MC, Pham N, ROTIN D. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J. Cell Biol. (2006) 175: 631-45.
  4. Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, ROTIN D. Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast. Mol. Syst. Biol.(2007) 3: 1-12.
  5. Muise AM, Walters T, Wine E, Griffiths AM, Turner D, Duerr RH, Regueiro MD, Ngan BY, Xu W, Sherman PM, Silverberg MS, ROTIN D. Protein-tyrosine phosphatase sigma is associated with ulcerative colitis. Curr. Biol. (2007) 17: 1212-18.
  6. Ing B, Shteiman-Kotler A, Castelli M, Henry P, Pak Y, Stewart B, Boulianne GL, ROTIN D. Regulation of Commissureless by the ubiquitin ligase DNedd4 is required for neuromuscular synaptogenesis in Mol. Cell Biol. (2007) 27: 481-96.
  7. Wiesner S, Ogunjimi AA, Wang HR, ROTIN D, Sicheri F, Wrana JL, Forman-Kay JD. Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain. Cell(2007) 130: 651-62.
  8. Lu C, Pribanic S, Debonneville A, Jiang C, ROTIN D. The PY motif of ENaC, mutated in Liddle syndrome, regulates channel internalization, sorting and mobilization from sub-apical pool. Traffic (2007) 8: 1246-64.
  9. Siu R, Fladd C, ROTIN D. N-cadherin is an in vivo substrate for protein tyrosine phosphatase sigma (PTPsigma) and participates in PTPsigma-mediated inhibition of axon growth. Mol. Cell Biol.(2007) 27: 208-19.
  10. Fouladkou F, Landry T, Kawabe H, Neeb A, Lu C, Brose N, Stambolic V, ROTIN D. The ubiquitin ligase Nedd4-1 is dispensable for the regulation of PTEN stability and localization. Proc. Natl. Acad. Sci. USA(2008) 105: 8585-90.
  11. Persaud A, Alberts P, Amsen EM, Xiong X, Wasmuth J, Saadon Z, Fladd C, Parkinson J, ROTIN D. Comparison of substrate specificity of the ubiquitin ligases Nedd4 and Nedd4-2 using proteome arrays. Mol. Syst. Biol.(2009) 5: 1-18.
  12. ROTIN D, Kumar S. Physiological functions of the HECT family of ubiquitin ligases. Nat. Rev. Mol. Cell Biol.(2009) 10: 398-409.
  13. Fouladkou F, Lu C, Jiang C, Zhou L, She Y, Walls JR, Kawabe H, Brose N, Henkelman RM, Huang A, Bruneau BG, ROTIN D. The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1. J. Biol. Chem.(2010) 285: 6770-80.

2011-2020

  1. Kimura T, Kawabe H, Jiang C, Zhang W, Xiang YY, Lu C, Salter MW, Brose N, Lu WY, ROTIN D. Deletion of the ubiquitin ligase Nedd4L in lung epithelia causes cystic fibrosis-like disease. Proc. Natl. Acad. Sci. USA(2011) 108: 3216-21.
  2. Persaud A, Alberts P, Hayes M, Guettler S, Clarke I, Sicheri F, Dirks P, Ciruna B, ROTIN D. Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function. EMBO J.(2011) 30: 3259-73.
  3. Trzcinska-Daneluti AM, Nguyen L, Jiang C, Fladd C, Uehling D, Prakesch M, Al-awar R, ROTIN D. Use of kinase inhibitors to correct ΔF508-CFTR function. Mol. Cell. Proteomics(2012) 11: 745-57.
  4. Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai AM, Neculai D, Vorobyov A, Garg P, Beatty L, Chan PK, Juang YC, Landry MC, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedadi M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, ROTIN D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS. A strategy for modulation of enzymes in the ubiquitin system. Science(2013) 339: 590-5.
  5. Beck J, Maerki S, Posch M, Metzger T, Persaud A, Scheel H, Hofmann K, ROTIN D, Pedrioli P, Swedlow JR, Peter M, Sumara I. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat. Cell Biol.(2013) 15: 430-9.
  6. Persaud A, Alberts P, Mari S, Tong J, Murchie R, Maspero E, Safi F, Moran MF, Polo S, ROTIN D. Tyrosine phosphorylation of NEDD4 activates its ubiquitin ligase activity. Sci. Signal.(2014) 7: ra95.
  7. Fang NN, Chan GT, Zhu M, Comyn SA, Persaud A, Deshaies RJ, ROTIN D, Gsponer J, Mayor T. Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat. Cell Biol.(2014) 16: 1227-37.
  8. Murchie R, Guo CH, Persaud A, Muise A, ROTIN D. Protein tyrosine phosphatase σ targets apical junction complex proteins in the intestine and regulates epithelial permeability. Proc. Natl. Acad. Sci. USA(2014) 111: 693-8.
  9. Milkereit R, Persaud A, Vanoaica L, Guetg A, Verrey F, ROTIN D. LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation. Nat. Comm.(2015) 6: 7250: 1-9.
  10. Trzcinska-Daneluti AM, Chen A, Nguyen L, Murchie R, Jiang C, Moffat J, Pelletier L, ROTIN D. RNA Interference Screen to Identify Kinases That Suppress Rescue of ΔF508-CFTR. Mol. Cell. Proteomics(2015) 14: 1569-83.
  11. Safi F, Shteiman-Kotler A, Zhong Y, Iliadi KG, Boulianne GL, ROTIN D. Drosophila Nedd4-long reduces Amphiphysin levels in muscles and leads to impaired T-tubule formation. Mol. Biol. Cell(2016) 27: 907-18.
  12. Zhang W, Wu K-P, Sartori MA, Kamadurai HB, Ordureau A, Jiang C, Mercredi PY, Murchie R, Hu J, Persaud A, Mukherjee M, Li N, Doye A, Walker JR, Sheng Y, Hao Z, Li Y, Brown KR, Lemichez E, Chen J, Tong Y, Harper JW, Moffat J, ROTIN D*, Schulman BA*, Sidhu SS*. System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes. Mol. Cell(2016) 62: 121-36. (*Co-Senior Authors)
  13. Lu C, Thoeni C, Connor A, Kawabe H, Gallinger S, ROTIN D. Intestinal knockout of Nedd4 enhances growth of APCmin tumors. Oncogene (2016) 35: 5839-49.
  14. Attali I, Tobelaim WS, Persaud A, Motamedchaboki K, Simpson-Lavy KJ, Mashahreh B, Levin-Kravets O, Keren-Kaplan T, Pilzer I, Kupiec M, Wiener R, Wolf DA, ROTIN D, Prag G. Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases. EMBO J.(2017) 36: 425-40.
  15. Jiang C, Kawabe H, ROTIN D. The Ubiquitin Ligase Nedd4L Regulates the Na/K/2Cl Co-transporter NKCC1/SLC12A2 in the Colon. J. Biol. Chem.(2017) 292: 3137-45.
  16. Demian WL, Persaud A, Jiang C, Coyaud É, Liu S, Kapus A, Kafri R, Raught B, ROTIN. The Ion Transporter NKCC1 Links Cell Volume to Cell Mass Regulation by Suppressing mTORC1. Cell Rep.(2019) 27: 1886-96.

2021-

  1. ROTIN D, Staub O. Function and regulation of the Epithelial Na Channel ENaC. Compr. Physiol.In Press, 2021