High-resolution high-throughput CryoEM

The High-Resolution High-Throughput CryoEM core is used for high-resolution structure determination of proteins that have already been screened at other facilities.

The facility provides state-of-the-art high-resolution imaging that can extend resolution from 4 to 6 Å to 2 to 4 Å. It does not provide an advantage for obtaining structures at less than 4 Å resolution, and it is not suitable for screening samples.

We facilitate high-resolution and high-throughput data collection for single particle electron cryomicroscopy of proteins and protein complexes. These data are collected with the ultimate goal of determining three-dimensional structures of protein and contributing to the development of drugs.

High-resolution high-throughput CryoEM

The High-Resolution High-Throughput CryoEM core is used for high-resolution structure determination of proteins that have already been screened at other facilities.

The facility provides state-of-the-art high-resolution imaging that can extend resolution from 4 to 6 Å to 2 to 4 Å. It does not provide an advantage for obtaining structures at less than 4 Å resolution, and it is not suitable for screening samples.

We house high-end microscopes with high-resolution and high-throughput data using cryo-electron microscopy single particle analysis, with the ultimate goal of producing three-dimensional structures of proteins to understand their function, and contribute to developing drugs that interact with it.

Fees

Service Rate per day
Internal users (SickKids/U of T Medicine/UHN/LTRI) with funding more than $150K external funding $785 (*)
Internal users (SickKids/U of T Medicine/UHN/LTRI) with funding less than $150K external funding $535 (*)
External academic user $2,035
External industry user $6,035
Consumables Rate per day (*)
One Autogrid (**) $40
Liquid nitrogen / day  $40

* Price decreases after 10 days

** A minimum of four autogrids are required for data collection

Interested in our services?

Users are asked to provide the following information in a single PDF file (less than 20 MB) to John Rubinstein and we’ll review your data collection request.

Section A

  • User name
  • Principal Investigator’s name
  • Institution: SickKids, UHN, U of T, LTRI, Other (please name)
  • Sample name and details

Section B

  • Describe where/how initial screening was done (Microscope, camera, pixel size, exposure and exposure rate)
  • How many particle images were obtained from each micrograph?
  • Attach a sample micrograph

Section C

  • Describe how initial processing was done (software, preprocessing details, number of micrographs, number of particle images used, FSC calculation details)
  • Attach images of initial 3D map and FSC curve

Section D

  • Does the sample contain material from primary human cells (Yes/No)?
  • If the answer for the question above is yes, please provide REB approval letter

Team

Dr. John Rubinstein, Facility Director
Dr. Samir Benlekbir, Facility Manager

Publications

  1. Courbon GM et al, EMBO J, doi: 10.15252/embj.2023113687 (2023)
  2. Di Trani JM et al, Proc Natl Acad Sci U S A, doi: 10.1073/pnas.2307093120 (2023)
  3. Wang H et al, Curr Opin Struct Biol, doi: 10.1016/j.sbi.2023.102592 (2023)
  4. Burn Aschner C et al, Sci Transl Med, doi: 10.1126/scitranslmed.adf4549 (2023)
  5. Liu RJY et al, J Biol Chem, doi: 10.1016/j.jbc.2023.104718 (2023)
  6. Liang Y et al, Proc Natl Acad Sci U S A, doi: 10.1073/pnas.2214949120 (2023)
  7. Xiao zheng Dou et al, Chemistry, DOI: 10.1002/chem.202300262 (2023)
  8. Wang H et al, Proc Natl Acad Sci U S A, doi: 10.1073/pnas.2217181120 (2023)
  9. Liang Y et al, Biochem Soc Trans, doi: 10.1042/BST20220611 (2023)
  10. Hasan SMN et al, Nat Commun, doi: 10.1038/s41467-023-39266-y (2023)
  11. Sarah C. Bickers et al, bioRxiv 2023.04.21.537812 (2023)
  12. Duc Minh Nguyen et al, bioRxiv 2023.09.20.558662 (2023)
  13. Morizumi T et al, Nat Commun, doi: 10.1038/s41467-023-40041-2 (2023)
  14. Kucharska I et al, PLoS Pathog, doi: 10.1371/journal.ppat.1010999 (2022)
  15. Tan YZ et al, Structure, doi: 10.1016/j.str.2022.07.006 (2022)
  16. Di Trani JM et al, Proc Natl Acad Sci U S A, doi: 10.1073/pnas.2205228119 (2022)
  17. Tan YZ et al, Life Sci Alliance, doi: 10.26508/lsa.202201527 (2022)
  18. Courbon GM et al, Front Microbiol, doi: 10.3389/fmicb.2022.864006 (2022)
  19. Vasanthakumar T et al, Nat Struct Mol Biol, doi: 10.1038/s41594-022-00757-z (2022)
  20. Guo H et al, Nat Commun, doi: 10.1038/s41467-022-29893-2 (2022)
  21. Keon KA et al, ACS Chem Biol, doi: 10.1021/acschembio.1c00894 (2022)
  22. Maxson ME et al, J Cell Biol, doi: 10.1083/jcb.202107174 (2022)
  23. Kucharska I et al, Elife, doi: 10.7554/eLife.72908 (2022)
  24. Moe A et al, Structure, doi: 10.1016/j.str.2021.11.008 (2022)
  25. Reisman BJ et al, Nat Chem Biol, doi: 10.1038/s41589-021-00900-9(2022)
  26. Harkness RW et al, Proc Natl Acad Sci U S A, doi: 10.1073/pnas.2109732118 (2021)
  27. McCallum M et al, Structure, doi: 10.1016/j.str.2020.11.019 (2021)
  28. Yanofsky DJ et al, Elife, 10:e71959 doi: 10.7554/eLife.71959 (2021)
  29. Miersch S et al, J Mol Biol, 433(19) doi: 10.1016/j.jmb.2021.167177 (2021)
  30. Di Trani JM et al, Structure, doi: 10.1016/j.str.2021.08.006 (2021)
  31. Rujas E et al, Nat Commun, 12(1):3661 doi: 10.1038/s41467-021-23825-2 (2021)
  32. Bickers SC et al, Proc Natl Acad Sci U S A, doi: 10.1073/pnas.2025853118 (2021)
  33. Yu C et al, Nat Commun, 12(1):3007 doi: 10.1038/s41467-021-23338-y (2021)
  34. Gorelik A et al, Sci Adv, 7(20) doi: 10.1126/sciadv.abf4155 (2021)
  35. Moe A et al, Proc Natl Acad Sci U S A, 118(11) doi: 10.1073/pnas.2021157118 (2021)
  36. Soroor F et al, Mol Biol Cell, 32(3):289-300. doi: 10.1091/mbc.E20-06-0398 (2021)
  37. Guo H et al, Nature, 589(7840):143-147 doi: 10.1038/s41586-020-3004-3 (2021)
  38. Lou JW et al, Commun Biol, doi: 10.1038/s42003-020-0997-y (2020)
  39. Ripstein ZA et al, J Am Chem Soc, doi: 10.1021/jacs.0c09474 (2020)
  40. Kucharska I et al, Elife, doi: 10.7554/eLife.59018 (2020)
  41. Tan YZ et al, Acta Crystallogr D Struct Biol, doi: 10.1107/S2059798320012474 (2020)
  42. Iga Kucharska et al, bioRxiv, doi: https://doi.org/10.1101/2020.06.02.131110 (2020)
  43. Yong Zi Tan et al, bioRxiv, doi: https://doi.org/10.1101/2020.05.03.075366 (2020)
  44. Hui Guo et al, bioRxiv, doi: https://doi.org/10.1101/2020.08.06.225375 (2020)
  45. Conicella AE et al, PNAS, volume 117, no 42, pages 26226-26236 (2020)
  46. Guo H et al, IUCrJ, volume7, no 5, pages 860-869 (2020)
  47. Huang R et al, Chem. Int. Ed. doi:10.1002/anie.202009767 (2020)
  48. Lee H et al, Cell, volume 182, no 2, pages 345-356 (2020)
  49. Abbas YM et al, Science, volume 367, no 6483, pages 1240-1246 (2020)
  50. Vahidi S et al, PNAS. volume 117, no 11, pages 5895-5906 (2020)
  51. Vasanthakumar T et al, Trends Biochem Sci, volume 45, no 4, pages 295-307 (2020)
  52. Ripstein ZA et al, eLife. 9:e52158. DOI: 10.7554/eLife.52158 (2020)
  53. Rubinstein JL et al, Acta Crystallogr D Struct Biol, v 75, no 12, p 1063-1070 (2019)
  54. McCallum M et al, Nat Commun, 10 (1):5198 (2019)
  55. Li Z, Tomlinson AC et al, Elife. 8: e51230 DOI: 10.7554/eLife.51230 (2019)
  56. Lou JW et al, Sci Rep. 9(1):12987 (2019)
  57. Lai LTF et al, Autophagy, DOI: 10.1080/15548627.2019.1639300 (2019)
  58. Guo H et al, eLife, 8:e43128 DOI: 10.7554/eLife.43128 (2019)
  59. Lou JW et al, bioRxiv 636167; doi: https://doi.org/10.1101/636167 (2019)
  60. Huang R et al, PNAS, volume 116, no 1, pages 158-167 (2019)
  61. Vasanthakumar T et al, PNAS, volume 116, no 15, pages 7272-7277 (2019)

  1. Edurne Rujas et al, bioRxiv 2020.10.15.341636; (2020)
  2. Iga Kucharska et al, bioRxiv 2020.06.02.131110; (2020)
  3. Yong Zi Tan et al, bioRxiv 2020.05.03.075366; (2020)
  4. Hui Guo et al, bioRxiv 2020.08.06.225375; (2020)
  5. Conicella AE et al, PNAS, volume 117, no 42, pages 26226-26236 (2020)
  6. Guo H et al, IUCrJ, volume7, no 5, pages 860-869 (2020)
  7. Huang R et al, Chem. Int. Ed. (2020)
  8. Lee H et al, Cell, volume 182, no 2, pages 345-356 (2020)
  9. Abbas YM et al, Science, volume 367, no 6483, pages 1240-1246 (2020)
  10. Vahidi S et al, PNAS. volume 117, no 11, pages 5895-5906 (2020)
  11. Vasanthakumar T et al, Trends Biochem Sci, volume 45, no 4, pages 295-307 (2020)
  12. Ripstein ZA et al, eLife. 9:e52158. (2020)
  13. Rubinstein JL et al, Acta Crystallogr D Struct Biol, volume 75, no 12, pages 1063-1070 (2019)
  14. McCallum M et al, Nat Commun, 10 (1):5198 (2019)
  15. Li Z, Tomlinson AC et al, Elife. 8: e51230 (2019)
  16. Lou JW et al, Sci Rep. 9(1):12987 (2019)
  17. Lai LTF et al, Autophagy, (2019)
  18. Guo H et al, eLife, 8:e43128 (2019)
  19. Lou JW et al, bioRxiv 636167; (2019)
  20. Huang R et al, PNAS, volume 116, no 1, pages 158-167 (2019)
  21. Vasanthakumar T et al, PNAS, volume 116, no 15, pages 7272-7277 (2019)
  22. Benjamin Wiseman et al, NSMB, volume 25, pages 1128–1136 (2018)
  23. Siavash Vahidi et al, PNAS, volume 115, no 28 pages 6447-6456 (2018)

Contact us

For questions or more information about our services, please contact Dr. Samir Benlekbir at sb@sickkids.ca.