High-resolution high-throughput CryoEM
The High-Resolution High-Throughput CryoEM core is used for high-resolution structure determination of proteins that have already been screened at other facilities.
The facility provides state-of-the-art high-resolution imaging that can extend resolution from 4 to 6 Å to 2 to 4 Å. It does not provide an advantage for obtaining structures at less than 4 Å resolution, and it is not suitable for screening samples.
We house high-end microscopes with high-resolution and high-throughput data using cryo-electron microscopy single particle analysis, with the ultimate goal of producing three-dimensional structures of proteins to understand their function, and contribute to developing drugs that interact with it.
High-resolution high-throughput CryoEM
The High-Resolution High-Throughput CryoEM core is used for high-resolution structure determination of proteins that have already been screened at other facilities.
The facility provides state-of-the-art high-resolution imaging that can extend resolution from 4 to 6 Å to 2 to 4 Å. It does not provide an advantage for obtaining structures at less than 4 Å resolution, and it is not suitable for screening samples.
We house high-end microscopes with high-resolution and high-throughput data using cryo-electron microscopy single particle analysis, with the ultimate goal of producing three-dimensional structures of proteins to understand their function, and contribute to developing drugs that interact with it.
Fees
Service | Rate per day (*) |
---|---|
Internal users (SickKids/U of T/UHN/Mt. Sinai) with funding higher than $150K | $785 |
Internal users (SickKids/U of T/UHN/Mt. Sinai) with funding less than $150K | $535 |
External Users Krios microscope | $785 |
Internal users (SickKids/U of T/UHN/Mt. Sinai) with funding less than $150K | $535 |
External | $2,035 |
Industrial | $6,035 |
Consumables | Rate per day (*) |
---|---|
One Autogrid (**) | $30 |
Liquid nitrogen / day | $35 |
* Price decreases after 10 days
** A minimum of four autogrids are required for data collection
Interested in our services?
Users are asked to provide the following information in a single PDF file (less than 20 MB) to John Rubinstein and we’ll review your data collection request.
Section A
- User name
- Principal Investigator’s name
- Institution: SickKids, UHN, U of T, LTRI, Other (please name)
- Sample name and details
Section B
- Describe where/how initial screening was done (Microscope, camera, pixel size, exposure and exposure rate)
- How many particle images were obtained from each micrograph?
- Attach a sample micrograph
Section C
- Describe how initial processing was done (software, preprocessing details, number of micrographs, number of particle images used, FSC calculation details)
- Attach images of initial 3D map and FSC curve
Team


Publications
- Edurne Rujas et al, bioRxiv 2020.10.15.341636; (2020)
- Iga Kucharska et al, bioRxiv 2020.06.02.131110; (2020)
- Yong Zi Tan et al, bioRxiv 2020.05.03.075366; (2020)
- Hui Guo et al, bioRxiv 2020.08.06.225375; (2020)
- Conicella AE et al, PNAS, volume 117, no 42, pages 26226-26236 (2020)
- Guo H et al, IUCrJ, volume7, no 5, pages 860-869 (2020)
- Huang R et al, Chem. Int. Ed. (2020)
- Lee H et al, Cell, volume 182, no 2, pages 345-356 (2020)
- Abbas YM et al, Science, volume 367, no 6483, pages 1240-1246 (2020)
- Vahidi S et al, PNAS. volume 117, no 11, pages 5895-5906 (2020)
- Vasanthakumar T et al, Trends Biochem Sci, volume 45, no 4, pages 295-307 (2020)
- Ripstein ZA et al, eLife. 9:e52158. (2020)
- Rubinstein JL et al, Acta Crystallogr D Struct Biol, volume 75, no 12, pages 1063-1070 (2019)
- McCallum M et al, Nat Commun, 10 (1):5198 (2019)
- Li Z, Tomlinson AC et al, Elife. 8: e51230 (2019)
- Lou JW et al, Sci Rep. 9(1):12987 (2019)
- Lai LTF et al, Autophagy, (2019)
- Guo H et al, eLife, 8:e43128 (2019)
- Lou JW et al, bioRxiv 636167; (2019)
- Huang R et al, PNAS, volume 116, no 1, pages 158-167 (2019)
- Vasanthakumar T et al, PNAS, volume 116, no 15, pages 7272-7277 (2019)
- Benjamin Wiseman et al, NSMB, volume 25, pages 1128–1136 (2018)
- Siavash Vahidi et al, PNAS, volume 115, no 28 pages 6447-6456 (2018)
- Edurne Rujas et al, bioRxiv 2020.10.15.341636; (2020)
- Iga Kucharska et al, bioRxiv 2020.06.02.131110; (2020)
- Yong Zi Tan et al, bioRxiv 2020.05.03.075366; (2020)
- Hui Guo et al, bioRxiv 2020.08.06.225375; (2020)
- Conicella AE et al, PNAS, volume 117, no 42, pages 26226-26236 (2020)
- Guo H et al, IUCrJ, volume7, no 5, pages 860-869 (2020)
- Huang R et al, Chem. Int. Ed. (2020)
- Lee H et al, Cell, volume 182, no 2, pages 345-356 (2020)
- Abbas YM et al, Science, volume 367, no 6483, pages 1240-1246 (2020)
- Vahidi S et al, PNAS. volume 117, no 11, pages 5895-5906 (2020)
- Vasanthakumar T et al, Trends Biochem Sci, volume 45, no 4, pages 295-307 (2020)
- Ripstein ZA et al, eLife. 9:e52158. (2020)
- Rubinstein JL et al, Acta Crystallogr D Struct Biol, volume 75, no 12, pages 1063-1070 (2019)
- McCallum M et al, Nat Commun, 10 (1):5198 (2019)
- Li Z, Tomlinson AC et al, Elife. 8: e51230 (2019)
- Lou JW et al, Sci Rep. 9(1):12987 (2019)
- Lai LTF et al, Autophagy, (2019)
- Guo H et al, eLife, 8:e43128 (2019)
- Lou JW et al, bioRxiv 636167; (2019)
- Huang R et al, PNAS, volume 116, no 1, pages 158-167 (2019)
- Vasanthakumar T et al, PNAS, volume 116, no 15, pages 7272-7277 (2019)
- Benjamin Wiseman et al, NSMB, volume 25, pages 1128–1136 (2018)
- Siavash Vahidi et al, PNAS, volume 115, no 28 pages 6447-6456 (2018)
Contact us
For questions or more information about our services, please contact Dr. Samir Benlekbir at sb@sickkids.ca.