No events in acquisition display and RUN button is green
What collection medium should the cells be sorted into?
We recommend 100% serum or HEPES buffered HBSS. The sorter uses PBS as its sheath fluid. Mixing large quantities of PBS with buffer containing calcium chloride may produce a precipitate if calcium phosphate on the cell membranes. This can adversely affect viability. For this reason, it is preferable to sort large numbers of cells [...]