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FAQs Archive

No events in acquisition display and RUN button is green

By nsiddiqui|2017-11-09T20:55:54+00:00November 9th, 2017|Troubleshooting|Comments Off

What collection medium should the cells be sorted into?

We recommend 100% serum or HEPES buffered HBSS. The sorter uses PBS as its sheath fluid. Mixing large quantities of PBS with buffer containing calcium chloride may produce a precipitate if calcium phosphate on the cell membranes. This can adversely affect viability. For this reason, it is preferable to sort large numbers of cells [...]

By nsiddiqui|2017-11-09T14:59:16+00:00November 9th, 2017|Cell sorting|Comments Off