Low event rate | Threshold level is too high | Lower the threshold level |
PMT voltage for the threshold parameter is set too low | Set the PMT voltage higher for the threshold parameter |
Sample is not adequately mixed | Mix the sample to suspend the cells |
Sample is too diluted | Concentrate the sample. If the flow rate setting is not critical to the application, set the flow rate switch to MED or HI |
Sample injection tube is clogged | Remove the sample tube to allow backflushing. If the event rate is still erratic, clean the sample injection tube |