ObservationPossible causesRecommended solutions
High CVAir bubble in sheath filter or flow cellPurge the filter
Sample flow rate is set too highSet the sample flow rate lower
Air leak at sheath containerEnsure that the sheath container lid is tight and all connectors are secure
Flow cell is dirtyPrime the instrument several times. Contact FMCF staff
Poor sample preparationRepeat sample preparation
Sample not diluted in same fluid as sheath fluidDilute the sample in the same fluid as you are using for sheath