| Observation | Possible causes | Recommended solutions |
|---|---|---|
| High CV | Air bubble in sheath filter or flow cell | Purge the filter |
| Sample flow rate is set too high | Set the sample flow rate lower | |
| Air leak at sheath container | Ensure that the sheath container lid is tight and all connectors are secure | |
| Flow cell is dirty | Prime the instrument several times. Contact FMCF staff | |
| Poor sample preparation | Repeat sample preparation | |
| Sample not diluted in same fluid as sheath fluid | Dilute the sample in the same fluid as you are using for sheath |
