ObservationPossible causesRecommended solutions
Poor QC resultsAir bubble or debris in flow cellPrime the fluidics system
Old or contaminated QC particlesMake new QC samples and perform the quality control procedure again
Sample not diluted in same fluid as sheath fluidDilute the sample in the same fluid as you are using for sheath
Laser not warmed upWait the recommended amount of time for the laser to warm up.
- 30 min for the 488-nm (blue)
- 30 min for the 355-nm (UV)
- 15 min for the 405-nm (violet)
- 20 min for the 633-nm (red)
Laser not functioningContact FMCF staff
Optical alignment problemContact FMCF staff