Distorted scatter parameters

Q: Distorted scatter parameters

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Observation	Possible causes	Recommended solutions
Distorted scatter parameters	Cytometer settings are improperly adjusted	Optimize the scatter parameters
	Air bubble in sheath filter or flow cell	Purge the air from the filter
	Flow cell is dirty	Perform the system flush procedure. Contact FMCF staff
	Air leak at sheath container	Ensure that the sheath container lid is tight and all connectors are secure
	Hypertonic buffers or fixative	Replace the buffers and fixative

nsiddiqui2017-11-09T20:56:34+00:00