No fluorescent signal

Q: No fluorescent signal

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Observation	Possible causes	Recommended solutions
No fluorescent signal	Incorrect fluorochrome assignment	Make sure the cytometer configuration in the software matches the optical filters in the cytometer
	Wrong filter is installed	Make sure the appropriate filter is installed for each fluorochrome
	Laser is not functioning	Verify the laser malfunction by changing the threshold to an alternative laser while running the appropriate sample. If unsuccessful, contact FMCF staff

nsiddiqui2017-11-09T20:56:07+00:00