Publications

2025

Wan, Y., Hudson, R., Smith, J., Forman-Kay^§, J.D., Ditlev, J.A.^§ Protein interactions, calcium, phosphorylation, and cholesterol modulate CFTR cluster formation on membranes. Proc. Nat. Acad, Sci. 2025; Mar 10; 122 (11): 1-12. doi.org/10.1073/pnas.2424470122.
Ravamehr-Lake, D., Hoveyda, S., Schlierf, M., Ditlev, J.A., Deber, C.M. Interaction of CFTR modulators with mammalian membrane mimetics: the role of cholesterol. Biochemistry. 2025; Mar 26; 64(8): 1878-1886. doi.org/10.1021/acs.biochem.4c00780.
Ditlev, J.A.^§, Forman-Kay, J.D.^§ Beyond peptide targeting sequences: machine learning of cellular condensate localization. Cell Res. 2025; Apr 14; 1-2. doi.org/10.1038/s41422-025-01115-6.
Arsenault, J., Kong, T., Saghian, R., Weng, O.Y., Pathak, S.S., Yang, C., Chao, O.Y., Rakhaminov, G., Forman-Kay, J.D., Ditlev, J.A.^§, Yang, Y.-M.^§, Wang, L.-Y.^§ Essential lipids enrich membrane associated condensates to rescue synaptic morpho-functional deficits in a mouse model of autism. Cell Reports. 2025; May 27; 44: 115573. doi.org/10.1016/jcelrep.2025.115573.
Akram, S.A., Chattaraj, A., Salava, T., Ditlev, J.A., Loew, L.M, Schmit, J.D. Biomolecular phase boundaries are described by a solubility product that accounts for variable stoichiometry and soluble oligomers. J Am Chem Soc. 2025 doi.org/10.1021/jacs.5c16034.

2024

Ahangama Liyanage, L., Ditlev, J.A. Mesoscale condensates organize the cytoplasm. Nat. Cell. Biol. 2024. Mar; 26: 310-312. doi.org/10.1038/s41556-023-01331-5.
Chattaraj, A.*, Baltaci, Z.*, Chung, S., Mayer, B.J., Loew, L.M., Ditlev, J.A. Measurement of solubility product reveals the interplay of oligomerization and self-association for defining condensate formation. Mol. Biol. Cell. 2024 Sept. 1; 35(9): 35:ar122, 1-11. doi.org/10.1091/mbc.E24-01-0030.
Ahangama Liyanage, L., McCready, F., Chung, S., Arsenault, J., Lin, X., Wang, L.Y., Ellis, J., Ditlev, J.A. Disease-linked mutation dysregulate neuronal condensate physical properties, composition, and RNA translation. BioRXiv. 2024. doi.org/10.1101/2024.11.01.621623.

2023

Wang, H.-Y., Chan, S.H., Dey, S., Castello-Serrano, I., Rosen, M.K., Ditlev, J.A., Levental, K.R., Levental, I. Coupling of protein condensates to ordered lipid domains determines functional membrane organization. Sci Adv. 2023 Apr 28;9(17):eadf6205. doi: 10.1126/sciadv.adf6205. Epub 2023 Apr 26. PMID: 37126554
Ahangama Liyanage, L., Rakhaminov, G., Chung, S., Ditlev, J.A. Plasma membrane shaping by protein phase separation. Plasma Membrane Shaping. 2023; 139-158. Textbook Chapter.

2022

Forman-Kay, J.D., Ditlev, J.A., Nosella, M.L., Lee, H.O. What are the distinguishing features and size requirements of biomolecular condensates and their implications for RNA-containing condensates? RNA. 2022. Jan 1:rna.079026.121. doi.org/10.1261/rna.079026.121.
Ditlev, J.A.Voices of the new generation: establishing yourself as a PI in a competitive field. Nat. Rev. Mol. Cell. Biol. 2021. Nov;22(11):710. doi.org/10.1038/s41580-021-00409-y.

2021

Zhemkov, V., Ditlev, J.A., Lee, W.R., Liou, J., Rosen, M.K., Bezprozvanny, I. The role of sigma-1 receptor in organization of endoplasmic reticulum signaling microdomains. ELife. 2021; May 11;10:e65192. doi.org/10.7554/eLife.65192
Ditlev, J.A.^§ Membrane-associated phase separation: organization and function emerge from a two-dimensional milieu. J. Mol. Cell. Biol. 2021 Feb. 3; doi.org/10.1093/jmcb/mjab010. Perspective.
Jaqaman, K.^§, Ditlev, J.A.^§ Biomolecular condensates in membrane receptor signaling. Curr. Opin. Cell Biol. 2021 April; 69: 48-54, doi.org/10.1016/j.ceb.2020.12.006. Review.

2020

Zhang, R.*, Lee, D.M.*, Jimah, J.R.*, Gerassimov, N., Yang, C., Kim, S., Luvsanjav, D., Winkelman, J., Mettlen, M., Abrams, M.E., Kalia, R., Keene, P., Pandey, P., Ravaux, B., Kim, J.H., Ditlev, J.A., Zhang, G., Rosen, M.K., Frost, A., Alto, N.M., Gardel, M., Schmid, S.L., Svitkina, T.M., Hinshaw, J.E., Chen, E.H. Dynamin regulates the dynamic and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein. Nat. Cell Biol. 2020; doi.org/10.1038/s41556-020-0519-7.

2003–2019

Ditlev, J.A.*, Vega, A. R.*, Köster, D.V.*, Su, X., Lakoduk, A.M., Vale, R.D., Mayor, S., Jaqaman, K., Rosen, M.K. A composition-dependent molecular clutch between T cell signaling clusters and actin. ELife. 2019; 8:e42695 doi.org/10.7554/elife.42695.
Case, L.B., Zhang, X., Ditlev, J.A., Rosen, M.K. Stoichiometry controls activity of phase separated clusters of actin signaling proteins. Science. 2019 Mar 8; 363(6431): 1093-1097. doi.org/10.1126/science.aau6313.
Case, L.B.^§, Ditlev, J.A.^§, Rosen, M.K.^§Regulation of transmembrane signaling by phase separation. Annu Rev Biophys.
Ditlev, J.A.^§, Case, L.B.^§, Rosen, M.K.^§ Who’s in and who’s out – Compositional control of biomolecular condensates. J. Mol. Biol. 2018 Nov 2; 430(23): 4666-4684. doi.org/10.1016/j.jmb.2018.08.003. Review.
Huang, W.Y.C*, Ditlev, J.A.*, Chiang, H.-K., Rosen, M.K., Groves, J.T. Allosteric modulation of Grb2 recruitment to the intrinsically unstructured scaffold protein, LAT, by remote site phosphorylation. J Am Chem Soc. 2017 Nov 28; 139 (49): 18009-18015. doi.org/10.1021/jacs.7b09387.
Su, X., Ditlev, J.A., Rosen, M.K., Vale, R.D. Reconstitution of TCR signaling using supported lipid bilayers. Methods Mol Biol. 2017;1584:65-76. doi.org/10.1007/978-1-4939-6881-7_5.
Su, X.*, Ditlev, J.A.*, Hui, E., Xing, W., Banjade, S., Okrut, J., King, D.S., Taunton, J., Rosen, M.K., Vale, R.D. Phase separation of signaling molecules promotes T cell receptor signal transduction. Science. 2016 Apr 29; 352(6285): 595-9. doi.org/10.1126/science.aad9964.
Ditlev, J.A., Mayer, B.J., Loew, L.M. There is more than one way to model and elephant. Experiment-driven modeling of the actin cytoskeleton. Biophys J. 2013 Feb 5; 104(3): 520-32. doi.org/10.1016/j.bpj.2012.12.044. Review.
Ditlev, J.A., Michalski, P.J., Huber, G., Rivera, G.M., Mohler, W.A., Loew, L.M., Mayer, B.J. Stoichiometry of Nck-dependent actin polymerization in living cells. J. Cell Biol. 2012 May 21; 197(5): 643-658. doi.org/10.1083/jcb.201111113.
Tan, M.H., Wong, C.F., Tan, H.L., Yang, X.J., Ditlev, J., Matsuda, D., Khoo, S.K., Sugimura, J., Fujioka, T., Furge, K.A., Kort, E., Giraud, S., Ferlicot, S., Vielh, P., Amsellem-Ouazana, D., Debré, B., Flam, T., Thiounn, N., Zerbib, M., Benoît, G., Droupy, S., Molinié, V., Vieillefond, A., Tan, P.H., Richard, S., Teh, B.T. Genomic expression and single-nucleotide polymorphism profiling discriminates chromophobe renal cell carcinoma and oncocytoma. BMC Cancer. 2010 May 12; 10:196. doi.org/10.1186/1471-2407-10-196.
Rogers, C.G., Ditlev, J.A., Tan, M.H., Sugimura, J., Qian, C.N., Cooper, J., Lane, B., Jewett, M.A., Kahnoski, R.J., Kort, E.J., Teh, B.T. Microarray gene expression profiling using core biopsies of renal neoplasia. Am J Transl Res. 2009 Jan 1; 1(1):55-61.
Ditlev, J.A., Vacanti, N.M., Novak, I.L., Loew, L.M. An open model of actin dendritic nucleation. Biophys J. 2009 May 6; 96(6): 3529-3542. doi.org/10.1016/j.bpj.2009.01.037.
Qian, C.N., Berghuis, B., Tsarfaty, G., Bruch, M., Kort, E.J., Ditlev, J., Tsarfaty, I., Hudson, E., Jackson, D.G., Petillo, D., Chen, J., Resau, J.H., Teh, B.T. Preparing the “soil”: the primary tumor induces vasculature reorganization in the sentinel lymph node before metastatic cancer cells. Cancer Res. 2006 Nov 1; 66(21):10365-76. doi.org/10.1158/0008-5472.CAN-06-2977.
Yang, X.J., Tan, M.H., Kim, H.L., Ditlev, J.A., Betten, M.W., Png, C.E., Kort, E.J., Futami, K., Furge, K.A., Takahashi, M., Kanayamo, H.O., Tan, P.H., Teh, B.S., Luan, C., Wang, K., Pins, M., Tretiakova, M., Anema, J., Kahnoski, R., Nicol, T., Stadler, W., Vogelzang, N.G., Amato, R., Seligson, D., Figlin, R., Belldegrun, A., Rogers, C.G., Teh, B.T. A molecular classification of papillary renal cell carcinoma. Cancer Res. 2005 Jul 1;65(13):5628-37. doi.org/10.1158/0008-5472.CAN-05-0533.
Ubels, J.L., Ditlev, J.A., Clousing, D.P., Casterton, P.L. Corneal permeability in a redesigned corneal holder for the bovine cornea opacity and permeability assay. Toxicol In Vitro. 2004 Dec; 18(6):853-7. doi.org/10.1016/j.tiv.2004.04.005.
Tan, M.H., Rogers, C.G., Cooper, J.T., Ditlev, J.A., Maatman, T.J., Yang, X., Furge, K.A., Teh, B.T. Gene expression profiling of renal cell carcinoma. Clin Cancer Res. 2004 Sep 15; 10 (18 Pt 2):6315S-21S. doi.org/10.1158/1078-0432.CCR-050002. Review.
Ubels, J.L., Veenstra, E., Ditlev, J., Ingersoll, K. Interactions of testosterone and all-trans retinoic acid in regulation of androgen receptor expression in rat lacrimal gland. Exp Eye Res. 2003 Dec; 77(6):741-8. doi.org/10.1016/j.exer.2003.07.006.