Custom services for generating zebrafish genetic variants and transgenic lines<\/p><\/div>
Custom services for generating zebrafish genetic variant and transgenic lines<\/p><\/div>
Based at The Hospital for Sick Children (SickKids) Research Institute<\/a>, the Zebrafish Genetics & Disease Models Core Facility offers a range of services to both internal and external researchers.<\/p>\nWe use contemporary mutagenesis and transgenesis techniques to generate zebrafish models of human disease in-house at our state-of-the-art facility. Zebrafish can be used to:<\/p>\n\nValidate candidate disease-causing genetic variations and provide insight into the molecular mechanisms underlying the disease<\/li>\nIdentify candidate therapeutics using high-throughput drug screens<\/li>\n<\/ul>\n<\/div><\/div><\/div><\/iframe><\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-5 fusion-flex-container has-pattern-background has-mask-background nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:50px;--awb-padding-bottom:0px;--awb-padding-bottom-small:0px;--awb-background-color:var(--awb-color1);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-7 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-7 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-text-color:var(--awb-custom_color_1);--awb-font-size:32px;\" id=\"services\"><h2 class=\"fusion-title-heading title-heading-left fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:400;margin:0;font-size:1em;--fontSize:32;line-height:2.19;\">Our services<\/h2><\/div><div class=\"fusion-separator\" style=\"align-self: flex-start;margin-right:auto;margin-bottom:35px;width:100%;max-width:150px;\"><div class=\"fusion-separator-border sep-wavy\" style=\"--awb-height:20px;--awb-amount:20px;--awb-sep-color:var(--awb-custom_color_3);--awb-separator-pattern-url:url("data:image\/svg+xml;utf8,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20preserveAspectRatio%3D%22none%22%20overflow%3D%22visible%22%20height%3D%22100%25%22%20viewBox%3D%220%200%2024%2024%22%20fill%3D%22none%22%20stroke%3D%22black%22%20stroke-width%3D%223%22%20stroke-linecap%3D%22square%22%20stroke-miterlimit%3D%2210%22%3E%3Cpath%20d%3D%22M0%2C6c6%2C0%2C0.9%2C11.1%2C6.9%2C11.1S18%2C6%2C24%2C6%22%2F%3E%3C%2Fsvg%3E");border-color:var(--awb-custom_color_3);\"><\/div><\/div><div class=\"fusion-text fusion-text-2 fusion-text-no-margin\" style=\"--awb-margin-bottom:50px;\"><div><strong>Please review the <a href=\"https:\/\/www.sickkids.ca\/siteassets\/research\/sickkids-core-facilities-terms-conditions.pdf\" target=\"_blank\" rel=\"noopener\">SickKids Core Facility Terms and Conditions (PDF)<\/a> before submitting a service request.<\/strong><\/div>\n<\/div><div class=\"fusion-tabs fusion-tabs-1 clean fusion-no-small-visibility vertical-tabs icon-position-left mobile-mode-toggle\" style=\"--awb-title-border-radius-top-left:0px;--awb-title-border-radius-top-right:0px;--awb-title-border-radius-bottom-right:0px;--awb-title-border-radius-bottom-left:0px;--awb-title-font-size:18px;--awb-alignment:center;--awb-inactive-color:var(--awb-custom_color_1);--awb-title-text-color:var(--awb-color1);--awb-title-active-text-color:var(--awb-custom_color_12);--awb-background-color:var(--awb-custom_color_2);--awb-border-color:rgba(235,234,234,0);--awb-active-border-color:#0066ad;--awb-title-font-family:"Lato";--awb-title-font-style:normal;--awb-title-font-weight:400;\"><div class=\"nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li class=\"active\" role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-aa53d6053f0d6c62f6a\" aria-selected=\"true\" id=\"fusion-tab-customzebrafishgeneticvariants\" href=\"#tab-aa53d6053f0d6c62f6a\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Custom zebrafish genetic variants<\/h3><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-55b176f1533e6781dc2\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-customtransgeniclines\" href=\"#tab-55b176f1533e6781dc2\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Custom transgenic lines<\/h3><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-1f519874e4c96c02c2a\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-morpholinos\" href=\"#tab-1f519874e4c96c02c2a\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Morpholinos <\/h3><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-563b6b6e7df204b2a19\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-zebrafishsmallmoleculescreens\" href=\"#tab-563b6b6e7df204b2a19\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Zebrafish small molecule screens<\/h3><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-24fdd5101bdf143be25\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-zebrafishimportation\" href=\"#tab-24fdd5101bdf143be25\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Zebrafish importation<\/h3><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-9c4639cd6484d1e8911\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-othercustomprojects\" href=\"#tab-9c4639cd6484d1e8911\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Other custom projects <\/h3><\/a><\/li><\/ul><\/div><div class=\"tab-content\"><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li class=\"active\" role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-aa53d6053f0d6c62f6a\" aria-selected=\"true\" id=\"mobile-fusion-tab-customzebrafishgeneticvariants\" href=\"#tab-aa53d6053f0d6c62f6a\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Custom zebrafish genetic variants<\/h3><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix in active\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-customzebrafishgeneticvariants\" id=\"tab-aa53d6053f0d6c62f6a\">\n<p>Using cutting-edge technology, we generate custom genetic variations in target genes.<\/p>\n<p>Based on project requirements, we can introduce a range of genetic variations, including:<\/p>\n<ul>\n<li>Small insertion-deletions resulting in frameshift and premature termination codons<\/li>\n<li>Large \u2018transcriptless\u2019 gene deletions<\/li>\n<li>Single nucleotide variants to mimic disease-associated genetic variations<\/li>\n<li>Knock-in fluorescent proteins or epitope tags into target genes<\/li>\n<\/ul>\n<p>Zebrafish lines can be shipped domestically or internationally to other zebrafish facilities for phenotypic analysis or studied at SickKids by establishing scientific collaborations facilitated by our core facility.<\/p>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-55b176f1533e6781dc2\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-customtransgeniclines\" href=\"#tab-55b176f1533e6781dc2\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Custom transgenic lines<\/h3><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-customtransgeniclines\" id=\"tab-55b176f1533e6781dc2\">We use modern molecular biology techniques, such as <strong>Gibson assembly<\/strong> and <strong>multi-site Gateway recombination<\/strong>, to rapidly assemble plasmids suitable for transgenesis and capable of expressing any protein of interest.<\/p>\n<p>We can perform microinjection of plasmids into one-cell stage zebrafish embryos and screen for germline transmission of transgenes to subsequent generations.<\/p>\n<p>Based on project requirements, we can generate a variety of transgenic lines, including:<\/p>\n<ul>\n<li>Fluorescent reporter lines to label cell lineages and\/or study the sub-cellular localization of proteins of interest<\/li>\n<li>Transgenes that can suppress genetic variant phenotypes<\/li>\n<li>Transgenes that allow for conditional spatial and temporal expression using the Cre\/Lox system<\/li>\n<li>Binary expression systems (sometimes referred to as <em>split systems<\/em>) that use either Gal4\/UAS or QF\/QUAS systems allowing for tissue specific expression<\/li>\n<\/ul>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-1f519874e4c96c02c2a\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-morpholinos\" href=\"#tab-1f519874e4c96c02c2a\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Morpholinos <\/h3><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-morpholinos\" id=\"tab-1f519874e4c96c02c2a\">Morpholinos can be used for transient knock-down experiments to study loss-of-function phenotypes in zebrafish embryos and larvae (up to five days post-fertilization).<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-563b6b6e7df204b2a19\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-zebrafishsmallmoleculescreens\" href=\"#tab-563b6b6e7df204b2a19\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Zebrafish small molecule screens<\/h3><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-zebrafishsmallmoleculescreens\" id=\"tab-563b6b6e7df204b2a19\">In collaboration with the <a href=\"https:\/\/lab.research.sickkids.ca\/sparc\/\" target=\"_blank\" rel=\"noopener\">SPARC BioCentre<\/a>, we can perform chemical screens to identify small molecules that can suppress a visible genetic variant phenotype and\/or alter the expression patterns of stable transgenic reporter lines.<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-24fdd5101bdf143be25\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-zebrafishimportation\" href=\"#tab-24fdd5101bdf143be25\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Zebrafish importation<\/h3><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-zebrafishimportation\" id=\"tab-24fdd5101bdf143be25\"><strong>*This service is temporarily unavailable.<\/strong><\/p>\n<p>Under the <em>Health of Animals Act<\/em>, zebrafish imported from outside Canada must be held in a Canada Food Inspection Agency (CFIA) pre-approved quarantine facility in order to be tested for spring viremia of carp virus (SVCV) before being released for research purposes.<\/p>\n<p>Our Zebrafish Quarantine Room is a CFIA-certified holding facility that allows us to import zebrafish from sources outside of Canada, on behalf of SickKids or other Canadian research laboratories.<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-9c4639cd6484d1e8911\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-othercustomprojects\" href=\"#tab-9c4639cd6484d1e8911\"><h3 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-angle-right fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Other custom projects <\/h3><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-othercustomprojects\" id=\"tab-9c4639cd6484d1e8911\">\n<p>Depending on your project needs and timelines, we offer customized projects not listed above on a case-by-case basis. <a href=\"\/zebrafish-core\/#contact\">Contact us for further inquiries<\/a>.<\/p>\n<\/div><\/div><\/div><div class=\"accordian fusion-accordian fusion-no-medium-visibility fusion-no-large-visibility\" style=\"--awb-border-size:0px;--awb-icon-size:15px;--awb-content-font-size:18px;--awb-icon-alignment:left;--awb-hover-color:var(--awb-custom_color_13);--awb-border-color:var(--awb-color2);--awb-background-color:rgba(0,102,173,0.05);--awb-divider-color:#e0dede;--awb-divider-hover-color:#e0dede;--awb-icon-color:var(--awb-color1);--awb-title-color:var(--awb-color8);--awb-content-color:var(--awb-color8);--awb-icon-box-color:var(--awb-custom_color_1);--awb-toggle-hover-accent-color:var(--awb-custom_color_1);--awb-toggle-active-accent-color:var(--awb-custom_color_1);--awb-title-font-family:"Lato";--awb-title-font-weight:400;--awb-title-font-style:normal;--awb-title-font-size:18px;--awb-content-font-family:Arial, Helvetica, sans-serif;--awb-content-font-style:normal;--awb-content-font-weight:400;\"><div class=\"panel-group fusion-toggle-icon-boxed\" id=\"accordion-1121-1\"><div class=\"fusion-panel panel-default panel-4eaf1f8cb875fcf84 fusion-toggle-no-divider fusion-toggle-boxed-mode\" style=\"--awb-title-color:var(--awb-custom_color_1);--awb-content-color:var(--awb-color8);\"><div class=\"panel-heading\"><h3 class=\"panel-title toggle\" id=\"toggle_4eaf1f8cb875fcf84\"><a aria-expanded=\"false\" aria-controls=\"4eaf1f8cb875fcf84\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-1121-1\" data-target=\"#4eaf1f8cb875fcf84\" href=\"#4eaf1f8cb875fcf84\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Custom zebrafish genetic variants<\/span><\/a><\/h3><\/div><div id=\"4eaf1f8cb875fcf84\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_4eaf1f8cb875fcf84\"><div class=\"panel-body toggle-content fusion-clearfix\">Using cutting-edge technology, we generate custom genetic variations in target genes.<\/p>\n<p>Based on project requirements, we can introduce a range of genetic variations, including:<\/p>\n<ul>\n<li>Small insertion-deletions resulting in frameshift and premature termination codons<\/li>\n<li>Large \u2018transcriptless\u2019 gene deletions<\/li>\n<li>Single nucleotide variants to mimic disease-associated genetic variations<\/li>\n<li>Knock-in fluorescent proteins or epitope tags into target genes<\/li>\n<\/ul>\n<p>Zebrafish lines can be shipped domestically or internationally to other zebrafish facilities for phenotypic analysis or studied at SickKids by establishing scientific collaborations facilitated by our core facility.<\/div><\/div><\/div><div class=\"fusion-panel panel-default panel-a91fa1df267cfb66d fusion-toggle-no-divider fusion-toggle-boxed-mode\" style=\"--awb-title-color:var(--awb-custom_color_1);\"><div class=\"panel-heading\"><h3 class=\"panel-title toggle\" id=\"toggle_a91fa1df267cfb66d\"><a aria-expanded=\"false\" aria-controls=\"a91fa1df267cfb66d\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-1121-1\" data-target=\"#a91fa1df267cfb66d\" href=\"#a91fa1df267cfb66d\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Custom transgenic lines<\/span><\/a><\/h3><\/div><div id=\"a91fa1df267cfb66d\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_a91fa1df267cfb66d\"><div class=\"panel-body toggle-content fusion-clearfix\">\n<p>We use modern molecular biology techniques, such as <strong>Gibson assembly<\/strong> and <strong>multi-site Gateway recombination<\/strong>, to rapidly assemble plasmids suitable for transgenesis and capable of expressing any protein of interest.<\/p>\n<p>We can perform microinjection of plasmids into one-cell stage zebrafish embryos and screen for germline transmission of transgenes to subsequent generations.<\/p>\n<p>Based on project requirements, we can generate a variety of transgenic lines, including:<\/p>\n<ul>\n<li>Fluorescent reporter lines to label cell lineages and\/or study the sub-cellular localization of proteins of interest<\/li>\n<li>Transgenes that can suppress genetic variant phenotypes<\/li>\n<li>Transgenes that allow for conditional spatial and temporal expression using the Cre\/Lox system<\/li>\n<li>Binary expression systems (sometimes referred to as <em>split systems<\/em>) that use either Gal4\/UAS or QF\/QUAS systems allowing for tissue specific expression<\/li>\n<\/ul>\n<\/div><\/div><\/div><div class=\"fusion-panel panel-default panel-fff32e7339cf4e269 fusion-toggle-no-divider fusion-toggle-boxed-mode\" style=\"--awb-title-color:var(--awb-custom_color_1);--awb-content-color:var(--awb-color8);\"><div class=\"panel-heading\"><h3 class=\"panel-title toggle\" id=\"toggle_fff32e7339cf4e269\"><a aria-expanded=\"false\" aria-controls=\"fff32e7339cf4e269\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-1121-1\" data-target=\"#fff32e7339cf4e269\" href=\"#fff32e7339cf4e269\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Morpholinos<\/span><\/a><\/h3><\/div><div id=\"fff32e7339cf4e269\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_fff32e7339cf4e269\"><div class=\"panel-body toggle-content fusion-clearfix\">\n<p>Morpholinos can be used for transient knock-down experiments to study loss-of-function phenotypes in zebrafish embryos and larvae (up to five days post-fertilization).<\/p>\n<\/div><\/div><\/div><div class=\"fusion-panel panel-default panel-d1aaa04b45fb9d292 fusion-toggle-no-divider fusion-toggle-boxed-mode\" style=\"--awb-title-color:var(--awb-custom_color_1);\"><div class=\"panel-heading\"><h3 class=\"panel-title toggle\" id=\"toggle_d1aaa04b45fb9d292\"><a aria-expanded=\"false\" aria-controls=\"d1aaa04b45fb9d292\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-1121-1\" data-target=\"#d1aaa04b45fb9d292\" href=\"#d1aaa04b45fb9d292\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Zebrafish small molecule screens<\/span><\/a><\/h3><\/div><div id=\"d1aaa04b45fb9d292\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_d1aaa04b45fb9d292\"><div class=\"panel-body toggle-content fusion-clearfix\">In collaboration with the <a href=\"https:\/\/lab.research.sickkids.ca\/sparc\/\" target=\"_blank\" rel=\"noopener\">SPARC BioCentre<\/a>, we can perform chemical screens to identify small molecules that can suppress a visible genetic variant phenotype and\/or alter the expression patterns of stable transgenic reporter lines.<\/div><\/div><\/div><div class=\"fusion-panel panel-default panel-445c4359580177607 fusion-toggle-no-divider fusion-toggle-boxed-mode\" style=\"--awb-title-color:var(--awb-custom_color_1);\"><div class=\"panel-heading\"><h3 class=\"panel-title toggle\" id=\"toggle_445c4359580177607\"><a aria-expanded=\"false\" aria-controls=\"445c4359580177607\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-1121-1\" data-target=\"#445c4359580177607\" href=\"#445c4359580177607\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Zebrafish importation<\/span><\/a><\/h3><\/div><div id=\"445c4359580177607\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_445c4359580177607\"><div class=\"panel-body toggle-content fusion-clearfix\">\n<p><strong>*This service is temporarily unavailable.<\/strong><\/p>\n<p>Under the <em>Health of Animals Act<\/em>, zebrafish imported from outside Canada must be held in a Canada Food Inspection Agency (CFIA) pre-approved quarantine facility in order to be tested for spring viremia of carp virus (SVCV) before being released for research purposes.<\/p>\n<p>Our Zebrafish Quarantine Room is a CFIA-certified holding facility that allows us to import zebrafish from sources outside of Canada, on behalf of SickKids or other Canadian research laboratories.<\/p>\n<\/div><\/div><\/div><div class=\"fusion-panel panel-default panel-b60a17187dc0efa8d fusion-toggle-no-divider fusion-toggle-boxed-mode\" style=\"--awb-title-color:var(--awb-custom_color_1);\"><div class=\"panel-heading\"><h3 class=\"panel-title toggle\" id=\"toggle_b60a17187dc0efa8d\"><a aria-expanded=\"false\" aria-controls=\"b60a17187dc0efa8d\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-1121-1\" data-target=\"#b60a17187dc0efa8d\" href=\"#b60a17187dc0efa8d\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Other custom projects<\/span><\/a><\/h3><\/div><div id=\"b60a17187dc0efa8d\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_b60a17187dc0efa8d\"><div class=\"panel-body toggle-content fusion-clearfix\">\n<p>Depending on your project needs and timelines, we offer customized projects not listed above on a case-by-case basis. <a href=\"https:\/\/lab.research.sickkids.ca\/zebrafish-core\/#contact\">Contact us for further inquiries<\/a>.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-8 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-8 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-text-color:var(--awb-custom_color_1);--awb-font-size:32px;\" id=\"pricing\"><h2 class=\"fusion-title-heading title-heading-left fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:400;margin:0;font-size:1em;--fontSize:32;line-height:2.19;\">Pricing<\/h2><\/div><div class=\"fusion-separator\" style=\"align-self: flex-start;margin-right:auto;margin-bottom:35px;width:100%;max-width:150px;\"><div class=\"fusion-separator-border sep-wavy\" style=\"--awb-height:20px;--awb-amount:20px;--awb-sep-color:var(--awb-custom_color_3);--awb-separator-pattern-url:url("data:image\/svg+xml;utf8,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20preserveAspectRatio%3D%22none%22%20overflow%3D%22visible%22%20height%3D%22100%25%22%20viewBox%3D%220%200%2024%2024%22%20fill%3D%22none%22%20stroke%3D%22black%22%20stroke-width%3D%223%22%20stroke-linecap%3D%22square%22%20stroke-miterlimit%3D%2210%22%3E%3Cpath%20d%3D%22M0%2C6c6%2C0%2C0.9%2C11.1%2C6.9%2C11.1S18%2C6%2C24%2C6%22%2F%3E%3C%2Fsvg%3E");border-color:var(--awb-custom_color_3);\"><\/div><\/div><div class=\"fusion-text fusion-text-3\"><p>All pricing shown is in Canadian dollars.<\/p>\n<\/div>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\">Service<\/th>\n<th align=\"left\">Internal pricing<\/th>\n<th align=\"left\">External pricing<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">Mutagenesis projects<\/td>\n<td align=\"left\">$7,000<\/td>\n<td align=\"left\">$9,900<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Transgenesis projects<\/td>\n<td align=\"left\">$6,000<\/td>\n<td align=\"left\">$8,500<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Sperm cryopreservation<\/td>\n<td align=\"left\">$250 per line<\/td>\n<td align=\"left\">$350 per line<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Zebrafish (5 adult breeding pairs or 100 embryos)<\/td>\n<td align=\"left\">NA<\/td>\n<td align=\"left\">$200 per line<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-6 fusion-flex-container has-pattern-background has-mask-background nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:50px;--awb-padding-bottom:100px;--awb-background-color:var(--awb-color1);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-9 fusion_builder_column_1_1 1_1 fusion-flex-column fusion-flex-align-self-center\" style=\"--awb-padding-top:2rem;--awb-padding-right:2rem;--awb-padding-bottom:2rem;--awb-padding-left:2rem;--awb-overflow:hidden;--awb-bg-color:#ffffff;--awb-bg-color-hover:#ffffff;--awb-bg-size:cover;--awb-box-shadow:0px 0px 5px 2px var(--awb-color2);;--awb-border-radius:0.5rem 0.5rem 0.5rem 0.5rem;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-9 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-text-color:var(--awb-custom_color_1);--awb-font-size:32px;\"><h2 class=\"fusion-title-heading title-heading-left fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:400;margin:0;font-size:1em;--fontSize:32;line-height:2.19;\">Why use zebrafish?<\/h2><\/div><div class=\"fusion-separator\" style=\"align-self: flex-start;margin-right:auto;margin-bottom:35px;width:100%;max-width:150px;\"><div class=\"fusion-separator-border sep-wavy\" style=\"--awb-height:20px;--awb-amount:20px;--awb-sep-color:var(--awb-custom_color_3);--awb-separator-pattern-url:url("data:image\/svg+xml;utf8,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20preserveAspectRatio%3D%22none%22%20overflow%3D%22visible%22%20height%3D%22100%25%22%20viewBox%3D%220%200%2024%2024%22%20fill%3D%22none%22%20stroke%3D%22black%22%20stroke-width%3D%223%22%20stroke-linecap%3D%22square%22%20stroke-miterlimit%3D%2210%22%3E%3Cpath%20d%3D%22M0%2C6c6%2C0%2C0.9%2C11.1%2C6.9%2C11.1S18%2C6%2C24%2C6%22%2F%3E%3C%2Fsvg%3E");border-color:var(--awb-custom_color_3);\"><\/div><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"width:104% !important;max-width:104% !important;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-0 fusion_builder_column_inner_2_3 2_3 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:66.666666666667%;--awb-margin-top-large:0px;--awb-spacing-right-large:2.88%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:2.88%;--awb-width-medium:66.666666666667%;--awb-order-medium:0;--awb-spacing-right-medium:2.88%;--awb-spacing-left-medium:2.88%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-4\"><p>Zebrafish possess several key attributes that make them a compelling vertebrate animal model system to study human disease:<\/p>\n<\/div><ul style=\"--awb-line-height:30.6px;--awb-icon-width:30.6px;--awb-icon-height:30.6px;--awb-icon-margin:12.6px;--awb-content-margin:43.2px;\" class=\"fusion-checklist fusion-checklist-1 fusion-checklist-default type-icons\"><li class=\"fusion-li-item\" style=\"\"><span class=\"icon-wrapper circle-no\"><i class=\"fusion-li-icon fa-check fas\" aria-hidden=\"true\"><\/i><\/span><div class=\"fusion-li-item-content\">Zebrafish are more<strong> cost-effective<\/strong> than generating and housing many alternative models (less than $1 per tank per day).<\/div><\/li><li class=\"fusion-li-item\" style=\"\"><span class=\"icon-wrapper circle-no\"><i class=\"fusion-li-icon awb-icon-check\" aria-hidden=\"true\"><\/i><\/span><div class=\"fusion-li-item-content\">\n<p>Juvenile zebrafish are transparent and develop ex-utero, allowing for easier internal imaging.<\/p>\n<\/div><\/li><li class=\"fusion-li-item\" style=\"\"><span class=\"icon-wrapper circle-no\"><i class=\"fusion-li-icon awb-icon-check\" aria-hidden=\"true\"><\/i><\/span><div class=\"fusion-li-item-content\">\n<p>Zebrafish share highly similar cell types, organ systems and physiology with humans and <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23594743\/\" target=\"_blank\" rel=\"noopener\">the majority (82%) of genes associated with human disease have a zebrafish counterpart<\/a>.<\/p>\n<\/div><\/li><li class=\"fusion-li-item\" style=\"\"><span class=\"icon-wrapper circle-no\"><i class=\"fusion-li-icon awb-icon-check\" aria-hidden=\"true\"><\/i><\/span><div class=\"fusion-li-item-content\">\n<p>A large number of fluorescent reporter lines are available marking major tissues and cell types and can be used to analyze phenotypic effects of genetic variants.<\/p>\n<\/div><\/li><li class=\"fusion-li-item\" style=\"\"><span class=\"icon-wrapper circle-no\"><i class=\"fusion-li-icon awb-icon-check\" aria-hidden=\"true\"><\/i><\/span><div class=\"fusion-li-item-content\">\n<p>Zebrafish can produce large clutches of <b>up to 300 embryos in a single pair breeding<\/b> and are amenable to high-throughput screens to identify small molecules capable of suppressing genetic variant\/disease phenotypes.<\/p>\n<\/div><\/li><\/ul><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-1 fusion_builder_column_inner_1_3 1_3 fusion-flex-column fusion-flex-align-self-stretch fusion-no-small-visibility\" style=\"--awb-bg-size:cover;--awb-width-large:33.333333333333%;--awb-margin-top-large:0px;--awb-spacing-right-large:5.76%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:5.76%;--awb-width-medium:33.333333333333%;--awb-order-medium:0;--awb-spacing-right-medium:5.76%;--awb-spacing-left-medium:5.76%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-center fusion-content-layout-column\"><div class=\"fusion-image-element \" style=\"--awb-aspect-ratio:1 \/ 1;--awb-object-position:54% 60%;--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-none imageframe-1 hover-type-none has-aspect-ratio\" style=\"border-radius:0.5rem;\"><img decoding=\"async\" width=\"1200\" height=\"1200\" alt=\"A person looking through a microscope.\" src=\"https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/zebrafish-facility-microscope.jpeg\" class=\"img-responsive wp-image-1260 img-with-aspect-ratio\" data-parent-fit=\"cover\" data-parent-container=\".fusion-image-element\" srcset=\"https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/zebrafish-facility-microscope-200x200.jpeg 200w, https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/zebrafish-facility-microscope-400x400.jpeg 400w, https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/zebrafish-facility-microscope-600x600.jpeg 600w, https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/zebrafish-facility-microscope-800x800.jpeg 800w, https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/zebrafish-facility-microscope.jpeg 1200w\" sizes=\"(max-width: 640px) 100vw, 400px\" \/><\/span><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-7 fusion-flex-container has-pattern-background has-mask-background nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:0px;--awb-padding-bottom:100px;--awb-background-color:var(--awb-color1);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-10 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-10 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-text-color:var(--awb-custom_color_1);--awb-font-size:32px;\" id=\"about\"><h2 class=\"fusion-title-heading title-heading-left fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:400;margin:0;font-size:1em;--fontSize:32;line-height:2.19;\">About us<\/h2><\/div><div class=\"fusion-separator\" style=\"align-self: flex-start;margin-right:auto;margin-bottom:35px;width:100%;max-width:150px;\"><div class=\"fusion-separator-border sep-wavy\" style=\"--awb-height:20px;--awb-amount:20px;--awb-sep-color:var(--awb-custom_color_3);--awb-separator-pattern-url:url("data:image\/svg+xml;utf8,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20preserveAspectRatio%3D%22none%22%20overflow%3D%22visible%22%20height%3D%22100%25%22%20viewBox%3D%220%200%2024%2024%22%20fill%3D%22none%22%20stroke%3D%22black%22%20stroke-width%3D%223%22%20stroke-linecap%3D%22square%22%20stroke-miterlimit%3D%2210%22%3E%3Cpath%20d%3D%22M0%2C6c6%2C0%2C0.9%2C11.1%2C6.9%2C11.1S18%2C6%2C24%2C6%22%2F%3E%3C%2Fsvg%3E");border-color:var(--awb-custom_color_3);\"><\/div><\/div><div class=\"fusion-text fusion-text-5\"><p>The Zebrafish Genetics & Disease Models Core Facility is the largest zebrafish facility in Canada. With decades of combined research experience, the team offers expertise in generating animal models of human disease, gene discovery, therapy development and more.<\/p>\n<p>To date, the facility has successfully generated over 80 mutant zebrafish lines for both internal and external clients. The facility also plays a key role in supporting groundbreaking research under SickKids\u2019 vision of <a href=\"https:\/\/www.sickkids.ca\/en\/about\/precision-child-health\/\" target=\"_blank\" rel=\"noopener\">Precision Child Health<\/a>.<\/p>\n<\/div><div ><a class=\"fusion-button button-flat fusion-button-default-size button-default fusion-button-default button-4 fusion-button-default-span fusion-button-default-type\" style=\"--button_margin-bottom:25px;\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.sickkids.ca\/en\/news\/archive\/2023\/specialized-facility-illuminates-genetic-mechanisms-underlying-human-health-conditions\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Read more about us on sickkids.ca<\/span><i class=\"fa-external-link-alt fas awb-button__icon awb-button__icon--default button-icon-right\" aria-hidden=\"true\"><\/i><\/a><\/div><div class=\"fusion-title title fusion-title-11 fusion-sep-none fusion-title-text fusion-title-size-three\" style=\"--awb-text-color:var(--awb-custom_color_1);--awb-margin-top:50px;--awb-margin-bottom:0px;--awb-font-size:24px;\"><h3 class=\"fusion-title-heading title-heading-left fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:400;margin:0;font-size:1em;--fontSize:24;--minFontSize:24;line-height:1.5;\">Team members<\/h3><\/div><div class=\"fusion-text fusion-text-6\"><ul>\n<li><strong>Jim Dowling<\/strong>, MD, PhD \u2013 Scientific Co-Director<\/li>\n<li><strong>Brian Ciruna<\/strong>, PhD \u2013 Scientific Co-Director<\/li>\n<li><a href=\"mailto:jason.burgess@sickkids.ca\"><strong>Jason Burgess<\/strong><\/a>, PhD \u2013 Service Manager<\/li>\n<li><strong>Xiucheng Cui<\/strong>, M.Sc. \u2013 Research Technologist<\/li>\n<li><strong>Rebecca Simonian<\/strong>, M.Sc. \u2013 Research Technologist<\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-bg-parallax\" data-bg-align=\"center top\" data-direction=\"left\" data-mute=\"false\" data-opacity=\"100\" data-velocity=\"-0.3\" data-mobile-enabled=\"false\" data-break_parents=\"0\" data-bg-image=\"https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/blue-green-waves-scaled.jpg\" data-bg-repeat=\"false\" data-bg-color=\"rgba(0,0,0,0.25)\" data-bg-color-small=\"var(--awb-custom_color_12)\" data-blend-mode=\"multiply\" data-blend-mode-small=\"multiply\" data-bg-gradient-type=\"linear\" data-bg-gradient-angle=\"180\" data-bg-gradient-start-color=\"rgba(255,255,255,0)\" data-bg-gradient-start-position=\"0\" data-bg-gradient-end-color=\"var(--awb-custom_color_1)\" data-bg-gradient-end-position=\"100\" data-bg-radial-direction=\"center center\" ><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-8 fusion-flex-container has-pattern-background has-mask-background fusion-parallax-left nonhundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-no-small-visibility fusion-no-medium-visibility\" style=\"--link_hover_color: var(--awb-color1);--link_color: var(--awb-color1);--awb-background-position:center top;--awb-background-blend-mode:multiply;--awb-background-blend-mode-small:multiply;--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:0px;--awb-padding-right:0px;--awb-padding-bottom:0px;--awb-padding-left:0px;--awb-padding-right-small:1rem;--awb-padding-left-small:1rem;--awb-min-height:calc( 100vh - 250px);--awb-background-color:rgba(0,0,0,0.25);--awb-background-color-small:var(--awb-custom_color_12);--awb-background-image:linear-gradient(180deg, rgba(255,255,255,0) 0%,var(--awb-custom_color_1) 100%),url(https:\/\/lab.research.sickkids.ca\/zebrafish-core\/wp-content\/uploads\/sites\/93\/2023\/11\/blue-green-waves-scaled.jpg);;--awb-background-size:cover;--awb-flex-wrap:wrap;\" id=\"contact\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-center fusion-flex-align-content-flex-start fusion-flex-justify-content-center fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-11 fusion_builder_column_1_1 1_1 fusion-flex-column fusion-flex-align-self-flex-end\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-section-separator section-separator waves-opacity fusion-section-separator-4\" style=\"--awb-spacer-padding-top:inherit;--awb-svg-margin-left:1.92%;--awb-svg-margin-right:1.92%;--awb-svg-margin-left-medium:1.92%;--awb-svg-margin-right-medium:1.92%;--awb-svg-margin-left-small:1.92%;--awb-svg-margin-right-small:1.92%;--awb-divider-height:200px;--awb-spacer-height:200px;--awb-sep-font-size:0;--awb-sep-line-height:0;\"><div class=\"fusion-section-separator-svg\"><svg class=\"fusion-waves-opacity-candy\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\" width=\"100%\" viewBox=\"0 1 1024 216\" preserveAspectRatio=\"none\" fill=\"rgba(255,255,255,1)\"><path class=\"st0\" d=\"M1024.1.4H.1L0 105.6c14.6 3.2 30.2 5.8 47.1 7.6 178.6 19.6 279.5-56.8 464.3-41.3 206.5 17.2 248.4 128.8 455 143.8 19 1.3 38.3.2 57.7-.3V.4z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st1\" d=\"M1024.1 196.4V.256H.1V80C22.8 86.4 48 91.4 76.3 94.6c178.6 19.8 272.2-53.9 457.1-38.4C740 73.4 730.7 180.9 937.3 196c27.2 2 56.6 2 86.8.4z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st2\" d=\"M1024.1.256H.1V36c45.1 15.4 95.2 27.7 150 33.7 178.6 19.8 220.6-46.8 405.4-31.3 206.6 17.2 197.8 114.7 404.4 129.7 20.4 1.5 42 2 64.2 1.7V.256z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st3\" d=\"M1024.1.156H.1V3.2c50.8 17.1 108.7 30.7 172.7 37.9 178.6 19.8 220-36.8 404.9-21.3 206.6 17.2 228 126.5 434.5 141.6 3.9.3 7.9.5 11.9.7V.156z\"\/>\n\t\t\t\t\t\t\t\t\t<\/svg><\/div><div class=\"fusion-section-separator-spacer\"><div class=\"fusion-section-separator-spacer-height\"><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-12 fusion_builder_column_2_3 2_3 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:66.666666666667%;--awb-margin-top-large:0px;--awb-spacing-right-large:2.88%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:2.88%;--awb-width-medium:66.666666666667%;--awb-order-medium:0;--awb-spacing-right-medium:2.88%;--awb-spacing-left-medium:2.88%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-12 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-two\" style=\"--awb-text-color:var(--awb-color1);--awb-font-size:64px;\"><h2 class=\"fusion-title-heading title-heading-center fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:700;margin:0;font-size:1em;--fontSize:64;line-height:1.1;\">Ready to get started?<\/h2><\/div><div class=\"fusion-text fusion-text-7\" style=\"--awb-text-color:var(--awb-color1);\"><p style=\"text-align: center\">Take advantage of our expertise in genome engineering and zebrafish biology to validate and advance your team’s understanding of human disease genes.<\/p>\n<p style=\"text-align: center\">To start your next project at the SickKids Zebrafish Core Facility, contact <a href=\"mailto:jason.burgess@sickkids.ca\">jason.burgess@sickkids.ca<\/a> for more information on our services.<\/p>\n<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-9 fusion-flex-container has-pattern-background has-mask-background nonhundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-no-small-visibility fusion-no-large-visibility\" style=\"--link_color: var(--awb-color1);--awb-background-position:center top;--awb-background-blend-mode:multiply;--awb-background-blend-mode-small:multiply;--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:0px;--awb-padding-right:0px;--awb-padding-bottom:0px;--awb-padding-left:0px;--awb-padding-right-small:1rem;--awb-padding-left-small:1rem;--awb-min-height:calc( 100vh - 250px);--awb-background-color-small:var(--awb-custom_color_12);--awb-background-image:linear-gradient(180deg, var(--awb-custom_color_2) 0%,var(--awb-custom_color_1) 50%);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-center fusion-flex-align-content-flex-start fusion-flex-justify-content-center fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-13 fusion_builder_column_1_1 1_1 fusion-flex-column fusion-flex-align-self-flex-end\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-section-separator section-separator waves-opacity fusion-section-separator-5\" style=\"--awb-spacer-padding-top:inherit;--awb-svg-margin-left:1.92%;--awb-svg-margin-right:1.92%;--awb-svg-margin-left-medium:1.92%;--awb-svg-margin-right-medium:1.92%;--awb-svg-margin-left-small:1.92%;--awb-svg-margin-right-small:1.92%;--awb-divider-height:200px;--awb-spacer-height:200px;--awb-sep-font-size:0;--awb-sep-line-height:0;\"><div class=\"fusion-section-separator-svg\"><svg class=\"fusion-waves-opacity-candy\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\" width=\"100%\" viewBox=\"0 1 1024 216\" preserveAspectRatio=\"none\" fill=\"rgba(255,255,255,1)\"><path class=\"st0\" d=\"M1024.1.4H.1L0 105.6c14.6 3.2 30.2 5.8 47.1 7.6 178.6 19.6 279.5-56.8 464.3-41.3 206.5 17.2 248.4 128.8 455 143.8 19 1.3 38.3.2 57.7-.3V.4z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st1\" d=\"M1024.1 196.4V.256H.1V80C22.8 86.4 48 91.4 76.3 94.6c178.6 19.8 272.2-53.9 457.1-38.4C740 73.4 730.7 180.9 937.3 196c27.2 2 56.6 2 86.8.4z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st2\" d=\"M1024.1.256H.1V36c45.1 15.4 95.2 27.7 150 33.7 178.6 19.8 220.6-46.8 405.4-31.3 206.6 17.2 197.8 114.7 404.4 129.7 20.4 1.5 42 2 64.2 1.7V.256z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st3\" d=\"M1024.1.156H.1V3.2c50.8 17.1 108.7 30.7 172.7 37.9 178.6 19.8 220-36.8 404.9-21.3 206.6 17.2 228 126.5 434.5 141.6 3.9.3 7.9.5 11.9.7V.156z\"\/>\n\t\t\t\t\t\t\t\t\t<\/svg><\/div><div class=\"fusion-section-separator-spacer\"><div class=\"fusion-section-separator-spacer-height\"><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-14 fusion_builder_column_2_3 2_3 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:66.666666666667%;--awb-margin-top-large:0px;--awb-spacing-right-large:2.88%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:2.88%;--awb-width-medium:66.666666666667%;--awb-order-medium:0;--awb-spacing-right-medium:2.88%;--awb-spacing-left-medium:2.88%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-13 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-two\" style=\"--awb-text-color:var(--awb-color1);--awb-font-size:64px;\"><h2 class=\"fusion-title-heading title-heading-center fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:700;margin:0;font-size:1em;--fontSize:64;line-height:1.1;\">Ready to get started?<\/h2><\/div><div class=\"fusion-text fusion-text-8\" style=\"--awb-text-color:var(--awb-color1);\"><p style=\"text-align: center\">Take advantage of our expertise in genome engineering and zebrafish biology to validate and advance your team’s understanding of human disease genes.<\/p>\n<p style=\"text-align: center\">To start your next project at the SickKids Zebrafish Core Facility, contact <a href=\"mailto:jason.burgess@sickkids.ca\">jason.burgess@sickkids.ca<\/a> for more information on our services.<\/p>\n<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-10 fusion-flex-container has-pattern-background has-mask-background nonhundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-no-medium-visibility fusion-no-large-visibility\" style=\"--link_color: var(--awb-color1);--awb-background-position:center top;--awb-background-blend-mode:multiply;--awb-background-blend-mode-small:multiply;--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:0px;--awb-padding-right:0px;--awb-padding-bottom:0px;--awb-padding-left:0px;--awb-padding-right-small:1rem;--awb-padding-bottom-small:3rem;--awb-padding-left-small:1rem;--awb-min-height:calc( 100vh - 250px);--awb-background-color-small:rgba(0,0,0,0);--awb-background-image:linear-gradient(180deg, var(--awb-custom_color_2) 0%,var(--awb-custom_color_1) 50%);--awb-flex-wrap:wrap;\" id=\"contact\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-center fusion-flex-align-content-flex-start fusion-flex-justify-content-center fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-15 fusion_builder_column_1_1 1_1 fusion-flex-column fusion-flex-align-self-flex-end\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-section-separator section-separator waves-opacity fusion-section-separator-6\" style=\"--awb-spacer-padding-top:inherit;--awb-svg-margin-left:1.92%;--awb-svg-margin-right:1.92%;--awb-svg-margin-left-medium:1.92%;--awb-svg-margin-right-medium:1.92%;--awb-svg-margin-left-small:1.92%;--awb-svg-margin-right-small:1.92%;--awb-divider-height:200px;--awb-spacer-height:200px;--awb-sep-font-size:0;--awb-sep-line-height:0;\"><div class=\"fusion-section-separator-svg\"><svg class=\"fusion-waves-opacity-candy\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\" width=\"100%\" viewBox=\"0 1 1024 216\" preserveAspectRatio=\"none\" fill=\"rgba(255,255,255,1)\"><path class=\"st0\" d=\"M1024.1.4H.1L0 105.6c14.6 3.2 30.2 5.8 47.1 7.6 178.6 19.6 279.5-56.8 464.3-41.3 206.5 17.2 248.4 128.8 455 143.8 19 1.3 38.3.2 57.7-.3V.4z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st1\" d=\"M1024.1 196.4V.256H.1V80C22.8 86.4 48 91.4 76.3 94.6c178.6 19.8 272.2-53.9 457.1-38.4C740 73.4 730.7 180.9 937.3 196c27.2 2 56.6 2 86.8.4z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st2\" d=\"M1024.1.256H.1V36c45.1 15.4 95.2 27.7 150 33.7 178.6 19.8 220.6-46.8 405.4-31.3 206.6 17.2 197.8 114.7 404.4 129.7 20.4 1.5 42 2 64.2 1.7V.256z\"\/>\n\t\t\t\t\t\t\t\t\t\t<path class=\"st3\" d=\"M1024.1.156H.1V3.2c50.8 17.1 108.7 30.7 172.7 37.9 178.6 19.8 220-36.8 404.9-21.3 206.6 17.2 228 126.5 434.5 141.6 3.9.3 7.9.5 11.9.7V.156z\"\/>\n\t\t\t\t\t\t\t\t\t<\/svg><\/div><div class=\"fusion-section-separator-spacer\"><div class=\"fusion-section-separator-spacer-height\"><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-16 fusion_builder_column_2_3 2_3 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:66.666666666667%;--awb-margin-top-large:0px;--awb-spacing-right-large:2.88%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:2.88%;--awb-width-medium:66.666666666667%;--awb-order-medium:0;--awb-spacing-right-medium:2.88%;--awb-spacing-left-medium:2.88%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-14 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-two\" style=\"--awb-text-color:var(--awb-color1);--awb-font-size:48px;\"><h2 class=\"fusion-title-heading title-heading-center fusion-responsive-typography-calculated\" style=\"font-family:"Lato";font-style:normal;font-weight:700;margin:0;font-size:1em;--fontSize:48;line-height:1.1;\">Ready to get started?<\/h2><\/div><div class=\"fusion-text fusion-text-9\" style=\"--awb-text-color:var(--awb-color1);\"><p style=\"text-align: center\">Take advantage of our expertise in genome engineering and zebrafish biology to validate and advance your team’s understanding of human disease genes.<\/p>\n<p style=\"text-align: center\">To start your next project at the SickKids Zebrafish Core Facility, contact <a href=\"mailto:jason.burgess@sickkids.ca\">jason.burgess@sickkids.ca<\/a> for more information on our services.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":385,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"100-width.php","meta":{"footnotes":""},"class_list":["post-1121","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ 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We use contemporary mutagenesis and transgenesis techniques to generate zebrafish models of human disease in-house at our state-of-the-art facility. Zebrafish can be used to:<\/p>\n