{"id":70,"date":"2017-09-15T15:33:55","date_gmt":"2017-09-15T15:33:55","guid":{"rendered":"http:\/\/localhost\/wordpress\/mass-spectrometry\/?page_id=70"},"modified":"2024-11-05T16:56:21","modified_gmt":"2024-11-05T16:56:21","slug":"cell-metabolism-analysis","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/","title":{"rendered":"Cell Metabolism (Seahorse) Analysis"},"content":{"rendered":"<div>\n<p><span style=\"color: #000000\">SPARC Molecular Analysis makes use of an<\/span> <span style=\"color: #307897\"><a style=\"color: #307897\" href=\"https:\/\/www.agilent.com\/en\/product\/cell-analysis\/real-time-cell-metabolic-analysis\/xf-analyzers\/seahorse-xfe96-analyzer-740879\" target=\"_blank\" rel=\"noopener\">Agilent Seahorse XFe96 Analyzer<\/a><\/span> <span style=\"color: #000000\">to interrogate in real-time the two major energy producing pathways of the cell, mitochondrial respiration and glycolysis. XF technology simultaneously measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), such that cellular functions like oxidative phosphorylation, glycolysis, and fatty acid oxidation, and their responses to substrates, inhibitors, or other compounds can be rapidly determined in a microplate format. The ability to measure the metabolic phenotype of cells and the shift between respiration and glycolysis under pathological conditions will allow you to connect physiological traits of cells with genomic and proteomic data. Researchers have successfully utilized XF technology to gain new insights into diabetes, obesity, cancer, mitochondrial diseases, cardiovascular and neurodegenerative function and more. For more information on XF technology, visit the<\/span> <span style=\"color: #307897\"><a style=\"color: #307897\" href=\"https:\/\/www.agilent.com\/\" target=\"_blank\" rel=\"noopener\">Agilent website<\/a>.<\/span><\/p>\n<p><span style=\"color: #000000\">Many of our users perform an\u00a0ATP production Rate Assay (ATP) or<\/span> <span style=\"color: #307897\"><a style=\"color: #307897\" href=\"https:\/\/www.agilent.com\/en-us\/products\/cell-analysis-(seahorse)\/mitochondrial-respiration-the-xf-cell-mito-stress-test?sh_0034\" target=\"_blank\" rel=\"noopener\">Mitochondrial Stress Test (MST)<\/a><\/span> <span style=\"color: #000000\">as their first-step, to measure key parameters of mitochondrial function and get a sense of whether further experimentation is warranted. Initially, the optimal cell density needs to be determined to ensure your data is within the optimal range of the XFe96, and FCCP concentration should be optimized for each cell type as well if you are performing a MST (see our<\/span> <a href=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/services\/cell-metabolism-analysis\/seahorse-faq\/\"><span style=\"color: #307897\">Seahorse FAQ<\/span><\/a> <span style=\"color: #000000\">to see how we typically do this). Users can also customize their Seahorse assay by, for example, varying cell conditions, injecting different compounds, or determining dose response curves.<\/span><\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #000000\"><strong>New Seahorse XF user or need a refresher?<\/strong> Check out the<\/span><span style=\"text-decoration: underline\"><strong><a href=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/11\/XF-FAS-Trainings-Flyer_DE41761884.pdf\"> Agilent Cell Analysis Virtual Seminars.<\/a><\/strong><\/span><\/p>\n<div>\n<p>&nbsp;<\/p>\n<p><strong>Ready to start a project? <\/strong><span style=\"color: #307897\"><a style=\"color: #307897\" href=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/submit-samples\/\">Submit your samples.<\/a><\/span><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>SPARC Molecular Analysis makes use of an Agilent Seahorse XFe96 Analyzer to interrogate in real-time the two major energy producing pathways of the cell, mitochondrial respiration and glycolysis. XF technology simultaneously measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), such that cellular functions like oxidative phosphorylation, glycolysis, and fatty acid oxidation, and their&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-70","page","type-page","status-publish","hentry","description-off"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Cell Metabolism (Seahorse) Analysis - SPARC BioCentre Molecular Analysis<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cell Metabolism (Seahorse) Analysis\" \/>\n<meta property=\"og:description\" content=\"SPARC Molecular Analysis makes use of an Agilent Seahorse XFe96 Analyzer to interrogate in real-time the two major energy producing pathways of the cell, mitochondrial respiration and glycolysis. 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