{"id":1921,"date":"2023-08-03T15:41:06","date_gmt":"2023-08-03T15:41:06","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/?page_id=1921"},"modified":"2025-10-07T15:28:29","modified_gmt":"2025-10-07T15:28:29","slug":"instrumentation-ms","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/","title":{"rendered":"Equipment"},"content":{"rendered":"

[vc_row][vc_column][vc_column_text]<\/p>\n

Liquid Chromatography Mass Spectrometry (LC-MS\/MS)<\/span><\/h3>\n

<\/h3>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1706808696330{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}”]\"\"<\/span>[\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]<\/p>\n

\n
\n
\n

QTRAP 6500+ with Exion HPLC<\/strong><\/span><\/p>\n<\/div>\n

\n

Acquired in 2019, the QTRAP 6500+ is our fastest and most sensitive instrument for small molecule LC-MS\/MS, providing the best limit of quantification (LOQ) for a broad scope of analytes. It is equipped with ESI and APCI ionization, as well as multiple scan modes, with a mass range of 40-1250 m\/z.<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1706808409634{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}” el_class=”msphoto”]\"\"[\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]<\/p>\n

\n

QTRAP 5500 with Agilent HPLC<\/strong><\/span><\/p>\n<\/div>\n

\n

Our two QTRAP 5500\u2019s are the workhorses of the lab, with established methods for many analytes including amino acids and their metabolites and neurotransmitters. Similar to the QTRAP 6500+, they are equipped with ESI and APCI ionization, multiple scan modes, and a mass range of 40-1250 m\/z.<\/span><\/p>\n<\/div>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1701981220327{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}”]\"\"<\/span>[\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]<\/p>\n

\n
\n
\n
\n

TSQ Altis<\/strong><\/span><\/p>\n

The TSQ Altis joined our fleet of mass spectrometers in September 2018. This triple quadrupole MS can quantify compounds at extremely low concentrations with high speed and sensitivity.<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1706810458102{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}”]\"\"<\/span>[\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]<\/p>\n

\n
\n

TripleTOF 6600 with Agilent HPLC<\/strong><\/span><\/p>\n<\/div>\n

\n

With a quadrupole time-of-flight (QTOF) mass analyzer, this instrument enables analysis of complex samples at high resolution, with a mass selection range of 30-2250 m\/z. It is our key instrument for lipidomics projects.<\/span><\/p>\n<\/div>\n<\/div>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Gas Chromatography (GC-MS)<\/h3>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1706810366502{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}”]\"\"[\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]<\/p>\n

\n
\n
\n
\n
\n

Agilent 7890A GC<\/strong><\/span><\/p>\n<\/div>\n

\n

The 7890A GC system is a state-of-the-art gas chromatograph that offers advanced separation capabilities and powerful productivity features. Its oven temperature control allows for fast and precise temperature ramping, providing optimal chromatography including peak symmetry and retention time repeatability.<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_column_text] Liquid Chromatography Mass Spectrometry (LC-MS\/MS) [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1706808696330{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}”][\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text] QTRAP 6500+ with Exion HPLC Acquired in 2019, the QTRAP 6500+ is our fastest and most sensitive…<\/p>\n","protected":false},"author":205,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1921","page","type-page","status-publish","hentry","description-off"],"yoast_head":"\nEquipment - SPARC BioCentre Molecular Analysis<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Equipment\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text] Liquid Chromatography Mass Spectrometry (LC-MS\/MS) [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1706808696330{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}”][\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text] QTRAP 6500+ with Exion HPLC Acquired in 2019, the QTRAP 6500+ is our fastest and most sensitive…\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/\" \/>\n<meta property=\"og:site_name\" content=\"SPARC BioCentre Molecular Analysis\" \/>\n<meta property=\"article:modified_time\" content=\"2025-10-07T15:28:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-scaled.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1920\" \/>\n\t<meta property=\"og:image:height\" content=\"2560\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/\",\"url\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/\",\"name\":\"Equipment - SPARC BioCentre Molecular Analysis\",\"isPartOf\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-768x1024.jpg\",\"datePublished\":\"2023-08-03T15:41:06+00:00\",\"dateModified\":\"2025-10-07T15:28:29+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#primaryimage\",\"url\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-scaled.jpg\",\"contentUrl\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-scaled.jpg\",\"width\":1920,\"height\":2560},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Equipment\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website\",\"url\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/\",\"name\":\"SPARC BioCentre Molecular Analysis\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Equipment - SPARC BioCentre Molecular Analysis","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/","og_locale":"en_US","og_type":"article","og_title":"Equipment","og_description":"[vc_row][vc_column][vc_column_text] Liquid Chromatography Mass Spectrometry (LC-MS\/MS) [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text css=”.vc_custom_1706808696330{margin-right: 98px !important;margin-left: 98px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;border-left-color: #000000 !important;border-left-style: solid !important;border-right-color: #000000 !important;border-right-style: solid !important;border-top-color: #000000 !important;border-top-style: solid !important;border-bottom-color: #000000 !important;border-bottom-style: solid !important;}”][\/vc_column_text][vc_column_text][\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text] QTRAP 6500+ with Exion HPLC Acquired in 2019, the QTRAP 6500+ is our fastest and most sensitive…","og_url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/","og_site_name":"SPARC BioCentre Molecular Analysis","article_modified_time":"2025-10-07T15:28:29+00:00","og_image":[{"width":1920,"height":2560,"url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-scaled.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/","url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/","name":"Equipment - SPARC BioCentre Molecular Analysis","isPartOf":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website"},"primaryImageOfPage":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#primaryimage"},"image":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#primaryimage"},"thumbnailUrl":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-768x1024.jpg","datePublished":"2023-08-03T15:41:06+00:00","dateModified":"2025-10-07T15:28:29+00:00","breadcrumb":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#primaryimage","url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-scaled.jpg","contentUrl":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2024\/02\/6500_IMG_9946-scaled.jpg","width":1920,"height":2560},{"@type":"BreadcrumbList","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/instrumentation-ms\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/"},{"@type":"ListItem","position":2,"name":"Equipment"}]},{"@type":"WebSite","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website","url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/","name":"SPARC BioCentre Molecular Analysis","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages\/1921","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/users\/205"}],"replies":[{"embeddable":true,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/comments?post=1921"}],"version-history":[{"count":33,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages\/1921\/revisions"}],"predecessor-version":[{"id":2339,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages\/1921\/revisions\/2339"}],"wp:attachment":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/media?parent=1921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}