{"id":156,"date":"2017-10-04T15:10:10","date_gmt":"2017-10-04T15:10:10","guid":{"rendered":"http:\/\/localhost\/wordpress\/molecular-analysis\/?page_id=156"},"modified":"2023-09-07T19:03:23","modified_gmt":"2023-09-07T19:03:23","slug":"how-do-i-get-started","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/","title":{"rendered":"How do I get started?"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]<a href=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/XF-official-training-part-1-slides_XF-assay-workflow_agilent.pdf\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-1999 size-full\" src=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png\" alt=\"\" width=\"1169\" height=\"871\" srcset=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png 1169w, https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps-300x224.png 300w, https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps-1024x763.png 1024w, https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps-768x572.png 768w\" sizes=\"auto, (max-width: 1169px) 100vw, 1169px\" \/><\/a>[\/vc_column_text][vc_column_text]The Agilent <strong>Seahorse<\/strong> <strong>XF Cell Mito Stress Test<\/strong> measures key parameters of mitochondrial function (basal respiration, ATP-linked respiration, maximal respiration, spare capacity, proton leak, non-mitochondrial oxygen consumption) by directly measuring the oxygen consumption rate (OCR) of cells.<\/p>\n<p>The Agilent <strong>Seahorse<\/strong> <strong>XF Real-Time ATP Rate Assay<\/strong> measures total ATP production rates in living cells and is able to distinguish between the fractions of ATP that are produced from mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, the two main metabolic pathways responsible for ATP production in mammalian cells.<\/p>\n<p>The Agilent <strong>Seahorse XF Glycolytic Rate Assay<\/strong> is an accurate and reliable analytical method for measuring glycolysis in cells. The assay utilizes both extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) measurements to determine the glycolytic proton efflux rate (glycoPER) of the cells.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_video link=&#8221;https:\/\/youtube.com\/embed\/XksRI5Y2fT4&#8243; el_width=&#8221;70&#8243; align=&#8221;center&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text][\/vc_column_text][vc_column_text]The Agilent Seahorse XF Cell Mito Stress Test measures key parameters of mitochondrial function (basal respiration, ATP-linked respiration, maximal respiration, spare capacity, proton leak, non-mitochondrial oxygen consumption) by directly measuring the oxygen consumption rate (OCR) of cells. The Agilent Seahorse XF Real-Time ATP Rate Assay measures total ATP production rates in living cells and is&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":70,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-156","page","type-page","status-publish","hentry","description-off"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>How do I get started? - SPARC BioCentre Molecular Analysis<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How do I get started?\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text][\/vc_column_text][vc_column_text]The Agilent Seahorse XF Cell Mito Stress Test measures key parameters of mitochondrial function (basal respiration, ATP-linked respiration, maximal respiration, spare capacity, proton leak, non-mitochondrial oxygen consumption) by directly measuring the oxygen consumption rate (OCR) of cells. The Agilent Seahorse XF Real-Time ATP Rate Assay measures total ATP production rates in living cells and is&hellip;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/\" \/>\n<meta property=\"og:site_name\" content=\"SPARC BioCentre Molecular Analysis\" \/>\n<meta property=\"article:modified_time\" content=\"2023-09-07T19:03:23+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1169\" \/>\n\t<meta property=\"og:image:height\" content=\"871\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/\",\"url\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/\",\"name\":\"How do I get started? - SPARC BioCentre Molecular Analysis\",\"isPartOf\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png\",\"datePublished\":\"2017-10-04T15:10:10+00:00\",\"dateModified\":\"2023-09-07T19:03:23+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#primaryimage\",\"url\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png\",\"contentUrl\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png\",\"width\":1169,\"height\":871},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Cell Metabolism (Seahorse) Analysis\",\"item\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"How do I get started?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website\",\"url\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/\",\"name\":\"SPARC BioCentre Molecular Analysis\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"How do I get started? - SPARC BioCentre Molecular Analysis","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/","og_locale":"en_US","og_type":"article","og_title":"How do I get started?","og_description":"[vc_row][vc_column][vc_column_text][\/vc_column_text][vc_column_text]The Agilent Seahorse XF Cell Mito Stress Test measures key parameters of mitochondrial function (basal respiration, ATP-linked respiration, maximal respiration, spare capacity, proton leak, non-mitochondrial oxygen consumption) by directly measuring the oxygen consumption rate (OCR) of cells. The Agilent Seahorse XF Real-Time ATP Rate Assay measures total ATP production rates in living cells and is&hellip;","og_url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/","og_site_name":"SPARC BioCentre Molecular Analysis","article_modified_time":"2023-09-07T19:03:23+00:00","og_image":[{"width":1169,"height":871,"url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/","url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/","name":"How do I get started? - SPARC BioCentre Molecular Analysis","isPartOf":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website"},"primaryImageOfPage":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#primaryimage"},"image":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#primaryimage"},"thumbnailUrl":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png","datePublished":"2017-10-04T15:10:10+00:00","dateModified":"2023-09-07T19:03:23+00:00","breadcrumb":{"@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#primaryimage","url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png","contentUrl":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-content\/uploads\/sites\/61\/2023\/09\/seahorse-assay-steps.png","width":1169,"height":871},{"@type":"BreadcrumbList","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/how-do-i-get-started\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/"},{"@type":"ListItem","position":2,"name":"Cell Metabolism (Seahorse) Analysis","item":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/cell-metabolism-analysis\/"},{"@type":"ListItem","position":3,"name":"How do I get started?"}]},{"@type":"WebSite","@id":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/#website","url":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/","name":"SPARC BioCentre Molecular Analysis","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages\/156","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/comments?post=156"}],"version-history":[{"count":5,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages\/156\/revisions"}],"predecessor-version":[{"id":1611,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages\/156\/revisions\/1611"}],"up":[{"embeddable":true,"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/pages\/70"}],"wp:attachment":[{"href":"https:\/\/lab.research.sickkids.ca\/sparc-molecular-analysis\/wp-json\/wp\/v2\/media?parent=156"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}