1.0SPARC BioCentre Molecular Analysishttps://lab.research.sickkids.ca/sparc-molecular-analysisjorgehttps://lab.research.sickkids.ca/sparc-molecular-analysis/author/jorge/Cell Metabolism (Seahorse) Analysisrich600338<blockquote class="wp-embedded-content" data-secret="dMKA1P0bjE"><a href="https://lab.research.sickkids.ca/sparc-molecular-analysis/cell-metabolism-analysis/">Cell Metabolism (Seahorse) Analysis</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://lab.research.sickkids.ca/sparc-molecular-analysis/cell-metabolism-analysis/embed/#?secret=dMKA1P0bjE" width="600" height="338" title="“Cell Metabolism (Seahorse) Analysis” — SPARC BioCentre Molecular Analysis" data-secret="dMKA1P0bjE" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script>
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SPARC Molecular Analysis makes use of an Agilent Seahorse XFe96 Analyzer to interrogate in real-time the two major energy producing pathways of the cell, mitochondrial respiration and glycolysis. XF technology simultaneously measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), such that cellular functions like oxidative phosphorylation, glycolysis, and fatty acid oxidation, and their…