{"id":6,"date":"2017-09-15T15:35:58","date_gmt":"2017-09-15T15:35:58","guid":{"rendered":"http:\/\/localhost\/wordpress\/hts\/?page_id=6"},"modified":"2023-04-10T19:38:00","modified_gmt":"2023-04-10T19:38:00","slug":"high-throughput-screening-hts","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/services\/high-throughput-screening-hts\/","title":{"rendered":"High-Throughput Screening (HTS) &amp; High-Content Analysis (HCA)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]<\/p>\n<div>The SPARC BioCentre offers access to automation platforms, high-throughput and high-content technologies, and guidance for High-Throughput Screening (HTS) assay development.<\/div>\n<div><\/div>\n<div>Our facility offers:<\/div>\n<ul>\n<li>small molecule high-throughput screening services on a cost recovery basis, to both SickKids and external investigators.<\/li>\n<li>small molecule libraries ranging in size from 320 to 69,000 compounds, for a total of 147,025 compounds (see a list of our collections).<\/li>\n<li>\u201cdrug-like\u201d small molecule collections and approved drug\/bioactive compound\/natural product collections.<\/li>\n<li>access to <em>in vivo<\/em> cell-based screening approaches to identify the role of specific genes in cell signaling using SPARC\u2019s extensive cDNA and shRNA libraries.<\/li>\n<li>guidance for HTS assay development and validation, for both biochemical and cell-based assays.<\/li>\n<li>access to \u201ctipless\u201d liquid dispensing instruments to reduce HTS costs and waste.<\/li>\n<li>support for analysis of HTS results and formation of a \u201chit\u201d list.<\/li>\n<li>access to CDD Vault software for analysis and visualization of HTS results.<\/li>\n<li>support for hit follow-up and confirmation.<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_video link=&#8221;https:\/\/www.youtube.com\/watch?v=8V7tosZzDtM&#8221; el_width=&#8221;80&#8243; align=&#8221;center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h4><\/h4>\n<h4><strong>Client Testimonials:<\/strong><\/h4>\n<p>\u201cOur specific project has required the development of novel HTS protocols. You\u2026have enthusiastically supported the development of these methods, and offered invaluable insight and assistance, and have greatly facilitated our research in developing an HTS screen.\u201d (Belinda Chang and Benjamin Scott, UT, June 2016)<\/p>\n<p>\u201c(You) have been instrumental in guiding me to choose and develop the optimal assay to use in our high-throughput screen and to select the most suitable small compound library for my purposes&#8230; I am very pleased with the excellent support and infrastructure SPARC BioCentre has to offer to perform high-throughput screens and subsequent confirmation experiments.<strong>\u201d<\/strong>\u00a0(Karin Hermans, SickKids, Aug 2016)<\/p>\n<p>\u201cI did a kinase inhibitor screen on\u2026cells using the Cellomics VTI with the SPARC facility to understand the pathways regulating\u2026these cells\u2026We were able to optimize the cell nuclear mask despite having very tightly clustered colonies and were able to automate the staining process, which allowed more consistency. From the screen, I was able to validate two known pathways and identify three novel ones.\u201d (CL, SickKids, June 2016)<\/p>\n<p><strong>For questions or inquiries regarding HTS of small molecule libraries, please contact Christopher Fladd; <\/strong><a href=\"mailto:rodolfo.gomez@sickkids.ca\">cfladd@sickkids.ca<\/a>.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<a href=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-5.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-198 size-medium\" src=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-5-300x225.jpg\" alt=\"Photo of the High-Throughput Screening Lab\" width=\"300\" height=\"225\" srcset=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-5-300x225.jpg 300w, https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-5-768x576.jpg 768w, https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-5-1024x768.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a> <a href=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-7.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-243\" src=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-7-225x300.jpg\" alt=\"Photo of Equipment\" width=\"168\" height=\"224\" srcset=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-7-225x300.jpg 225w, https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/multimedia-7-768x1024.jpg 768w\" sizes=\"auto, (max-width: 168px) 100vw, 168px\" \/><\/a>\u00a0<a href=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/hts-services-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-248\" src=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/hts-services-1-300x296.jpg\" alt=\"Photo of the High-Throughput Screening Lab\" width=\"226\" height=\"223\" srcset=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/hts-services-1-300x296.jpg 300w, https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/hts-services-1-768x758.jpg 768w, https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/wp-content\/uploads\/sites\/63\/2017\/11\/hts-services-1-1024x1010.jpg 1024w\" sizes=\"auto, (max-width: 226px) 100vw, 226px\" \/><\/a>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text] The SPARC BioCentre offers access to automation platforms, high-throughput and high-content technologies, and guidance for High-Throughput Screening (HTS) assay development. Our facility offers: small molecule high-throughput screening services on a cost recovery basis, to both SickKids and external investigators. small molecule libraries ranging in size from 320 to 69,000 compounds, for a total of&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":81,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-6","page","type-page","status-publish","hentry","description-off"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>High-Throughput Screening (HTS) &amp; High-Content Analysis (HCA) - SPARC Drug Discovery<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/services\/high-throughput-screening-hts\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"High-Throughput Screening (HTS) &amp; High-Content Analysis (HCA)\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text] The SPARC BioCentre offers access to automation platforms, high-throughput and high-content technologies, and guidance for High-Throughput Screening (HTS) assay development. 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