{"id":26,"date":"2017-09-15T15:41:17","date_gmt":"2017-09-15T15:41:17","guid":{"rendered":"http:\/\/localhost\/wordpress\/hts\/?page_id=26"},"modified":"2025-07-10T13:17:22","modified_gmt":"2025-07-10T13:17:22","slug":"lentivirus-ordering-information","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/services\/lentivirus\/lentivirus-ordering-information\/","title":{"rendered":"Lentivirus Ordering Information"},"content":{"rendered":"
\n

How to place an order:<\/h4>\n<\/div>\n

SickKids investigators are\u00a0REQUIRED<\/em><\/strong>\u00a0to have an approved biosafety protocol before virus particles can be released.<\/p>\n

External customers must provide documentation from their institution demonstrating approval to work with lentivirus before particles can be released.<\/p>\n

A Transporation of Dangerous Goods Certificate must be provided before transporting Lenti off-site.<\/p>\n

All Lenti Orders must be place through SPARC Drug Discovery QReserve<\/a> and include the SPARC Viral Particle Request Form<\/a><\/p>\n

Please direct any questions about Lentivirus to Chris Fladd cfladd@sickkids.ca<\/a> &\/or Jonathan Sayewich jonathan.sayewich@sickkids.ca<\/a><\/p>\n

The Plasmid DNA<\/h4>\n
    \n
  1. The DNA should be delivered in a sealed 1.5 mL microtube, clearly labeled as described above, including concentration<\/li>\n
  2. The DNA should be purified by maxi\/midi columns.\u00a0(mini-preps are not suitable)<\/strong><\/em><\/li>\n
  3. For small scale production 50ug of DNA is required for each T-75 flask (~10mL of viral supernatant) vector prep. Concentration should be 1.0ug\/uL<\/li>\n
  4. For large scale production 250ug of DNA is required (~50mL of viral supernatant) vector prep. Concentration should be 1.0ug\/uL<\/li>\n<\/ol>\n

    Picking up Viral Particles<\/h4>\n
      \n
    1. You will get an email message indicating the time to pick up your order<\/li>\n
    2. Viral stock(s) will be stored at \u00a0-80 C \u00a0or 4 C at the request of the customer<\/li>\n
    3. If determination of the titre is requested a\u00a0minimum of five days<\/strong><\/em>\u00a0is required after harvesting supernatant<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

      How to place an order: SickKids investigators are\u00a0REQUIRED\u00a0to have an approved biosafety protocol before virus particles can be released. External customers must provide documentation from their institution demonstrating approval to work with lentivirus before particles can be released. A Transporation of Dangerous Goods Certificate must be provided before transporting Lenti off-site. All Lenti Orders must…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":22,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-26","page","type-page","status-publish","hentry","description-off"],"yoast_head":"\nLentivirus Ordering Information - SPARC Drug Discovery<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/sparc-drug-discovery\/services\/lentivirus\/lentivirus-ordering-information\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Lentivirus Ordering Information\" \/>\n<meta property=\"og:description\" content=\"How to place an order: SickKids investigators are\u00a0REQUIRED\u00a0to have an approved biosafety protocol before virus particles can be released. 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