{"id":2305,"date":"2024-05-16T17:20:07","date_gmt":"2024-05-16T17:20:07","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/sbc-facility\/?page_id=2305"},"modified":"2025-04-01T14:15:23","modified_gmt":"2025-04-01T14:15:23","slug":"field-flow-fractionation-mals","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sbc-facility\/field-flow-fractionation-mals\/","title":{"rendered":"Field Flow Fractionation-MALS"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-overflow:visible;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_2_5 2_5 fusion-two-fifth fusion-column-first\" style=\"--awb-bg-size:cover;width:37.6%; margin-right: 4%;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-image-element in-legacy-container\" style=\"--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-dropshadow imageframe-1 hover-type-none\" style=\"-webkit-box-shadow: 3px 3px 7px rgba(0,0,0,0.3);box-shadow: 3px 3px 7px rgba(0,0,0,0.3);\"><img decoding=\"async\" width=\"292\" height=\"300\" title=\"FFF-MALS\" src=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/04\/FFF-MALS-292x300.jpg\" alt class=\"img-responsive wp-image-2306\" srcset=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/04\/FFF-MALS-200x205.jpg 200w, https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/04\/FFF-MALS-400x411.jpg 400w, https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/04\/FFF-MALS-600x616.jpg 600w, https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/04\/FFF-MALS.jpg 642w\" sizes=\"(max-width: 800px) 100vw, 600px\" \/><\/span><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_3_5 3_5 fusion-three-fifth fusion-column-last\" style=\"--awb-bg-size:cover;width:58.4%;\" data-scroll-devices=\"small-visibility,medium-visibility,large-visibility\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-1\"><h1>FFF-MALS<\/h1>\n<h2>Bio-Inert High Performance Liquid Chromatography with Field-Flow Fractionation, Multi-angle Static Light Scattering, Dynamic Light Scattering and Refractive Index (Bio-HPLC-FFF-MALS-DLS-RI)<\/h2>\n<h3>Agilent Technologies Bio-Inert HPLC and Wyatt Technology FFF-MALS-DLS-RI<\/h3>\n<hr \/>\n<h1>\u00a0Bio-Inert HPLC<\/h1>\n<ul>\n<li>Agilent\u00a01260 Infinity II Bio-Inert LC System using non-reactive materials<\/li>\n<li>Room temperature or component refrigerated<\/li>\n<li>Coupled to Wyatt FFF-MALS-DLS-RI<\/li>\n<li>HPLC system includes:\n<ul>\n<li>Quaternary Pump (0.001 &#8211; 10 mL\/min), max operating pressure 600 bar<\/li>\n<li>Multisampler \/ Autosampler (vials and multi-well plates)<\/li>\n<li>Bio-inert manual injector for larger injection volumes<\/li>\n<li>Peltier-controlled column compartment<\/li>\n<li>UV-VIS diode array detector<\/li>\n<li>Fluorescence diode array detector<\/li>\n<li>Fraction collector (glass vials \/ test tubes and 96-well, shallow and deep-well plates)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-1 fusion-button-default-span fusion-button-default-type fusion-animated\" style=\"--button_accent_color:#ffffff;--button_accent_hover_color:#64a0c8;--button_border_hover_color:#64a0c8;--button_border_width-top:1px;--button_border_width-right:1px;--button_border_width-bottom:1px;--button_border_width-left:1px;--button_gradient_top_color:#000000;--button_gradient_bottom_color:#000000;--button_gradient_top_color_hover:#ffffff;--button_gradient_bottom_color_hover:#ffffff;\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.1\" data-animationOffset=\"top-into-view\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"http:\/\/www.agilent.com\/#\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Learn more about the 1260 Infinity II Bio-Inert HPLC<\/span><\/a><\/div><div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-2 fusion-button-default-span fusion-button-default-type fusion-animated\" style=\"--button_accent_color:#ffffff;--button_accent_hover_color:#64a0c8;--button_border_hover_color:#64a0c8;--button_border_width-top:1px;--button_border_width-right:1px;--button_border_width-bottom:1px;--button_border_width-left:1px;--button_gradient_top_color:#000000;--button_gradient_bottom_color:#000000;--button_gradient_top_color_hover:#ffffff;--button_gradient_bottom_color_hover:#ffffff;\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.1\" data-animationOffset=\"top-into-view\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/get.qreserve.com\/#\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Reserve<\/span><\/a><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:20px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div><\/p>\n<h1>\u00a0FFF-MALS-DLS-RI<\/h1>\n<ul>\n<li>Field-Flow Fractionation, Multi-angle Static Light Scattering, Dynamic Light Scattering and Refractive Index Detection System<\/li>\n<li>Size exclusion chromatography (SEC-MALS) available<\/li>\n<li>Wyatt Technology Eclipse (FFF) with Dilution Control Module<\/li>\n<li>Wyatt Technology DAWN (18-angle MALS and DLS)<\/li>\n<li>Wyatt Technology Optilab (RI)<\/li>\n<li>Eclipse Short Channel, 350 \u00b5M and 500 \u00b5M fixed heights<\/li>\n<li>Eclipse Semi-preparative Channel, variable heights<\/li>\n<li>Refrigerated detection system<\/li>\n<li>Separation and characterization of macromolecules and nanoparticles from 1 to 1,000 nm in size<\/li>\n<li>Macromolecule molecular weight determination<\/li>\n<li>Multimeric state, shape, polydispersity<\/li>\n<\/ul>\n<p><div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-3 fusion-button-default-span fusion-button-default-type fusion-animated\" style=\"--button_accent_color:#ffffff;--button_accent_hover_color:#64a0c8;--button_border_hover_color:#64a0c8;--button_border_width-top:1px;--button_border_width-right:1px;--button_border_width-bottom:1px;--button_border_width-left:1px;--button_gradient_top_color:#000000;--button_gradient_bottom_color:#000000;--button_gradient_top_color_hover:#ffffff;--button_gradient_bottom_color_hover:#ffffff;\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.1\" data-animationOffset=\"top-into-view\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"http:\/\/www.wyatt.com\/products\/instruments\/eclipse-field-flow-fractionation-system.html\/#\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Learn more about the Eclipse<\/span><\/a><\/div><div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-4 fusion-button-default-span fusion-button-default-type fusion-animated\" style=\"--button_accent_color:#ffffff;--button_accent_hover_color:#64a0c8;--button_border_hover_color:#64a0c8;--button_border_width-top:1px;--button_border_width-right:1px;--button_border_width-bottom:1px;--button_border_width-left:1px;--button_gradient_top_color:#000000;--button_gradient_bottom_color:#000000;--button_gradient_top_color_hover:#ffffff;--button_gradient_bottom_color_hover:#ffffff;\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.1\" data-animationOffset=\"top-into-view\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"http:\/\/www.wyatt.com\/products\/instruments\/dawn-multi-angle-light-scattering-detector.html\/#\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Learn more about the DAWN<\/span><\/a><\/div><div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-5 fusion-button-default-span fusion-button-default-type fusion-animated\" style=\"--button_accent_color:#ffffff;--button_accent_hover_color:#64a0c8;--button_border_hover_color:#64a0c8;--button_border_width-top:1px;--button_border_width-right:1px;--button_border_width-bottom:1px;--button_border_width-left:1px;--button_gradient_top_color:#000000;--button_gradient_bottom_color:#000000;--button_gradient_top_color_hover:#ffffff;--button_gradient_bottom_color_hover:#ffffff;\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.1\" data-animationOffset=\"top-into-view\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"http:\/\/www.wyatt.com\/products\/instruments\/optilab-refractive-index-detector.html\/#\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Learn more about the OptiLab<\/span><\/a><\/div><div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-6 fusion-button-default-span fusion-button-default-type fusion-animated\" style=\"--button_accent_color:#ffffff;--button_accent_hover_color:#64a0c8;--button_border_hover_color:#64a0c8;--button_border_width-top:1px;--button_border_width-right:1px;--button_border_width-bottom:1px;--button_border_width-left:1px;--button_gradient_top_color:#000000;--button_gradient_bottom_color:#000000;--button_gradient_top_color_hover:#ffffff;--button_gradient_bottom_color_hover:#ffffff;\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.1\" data-animationOffset=\"top-into-view\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/get.qreserve.com\/#\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Reserve<\/span><\/a><\/div><\/p>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":116,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2305","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Field Flow Fractionation-MALS - The SBC Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/field-flow-fractionation-mals\/\" \/>\n<meta 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