{"id":180,"date":"2016-09-22T18:26:13","date_gmt":"2016-09-22T18:26:13","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/sbc-facility\/?page_id=180"},"modified":"2020-11-17T17:00:45","modified_gmt":"2020-11-17T17:00:45","slug":"microscope","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sbc-facility\/microscope\/","title":{"rendered":"Bright Field and Fluorescence Microscope with Thermal Stage"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-overflow:visible;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_2 1_2 fusion-one-half fusion-column-first\" style=\"--awb-bg-size:cover;width:48%; margin-right: 4%;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-image-element in-legacy-container\" style=\"--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-dropshadow imageframe-1 hover-type-none\" style=\"-webkit-box-shadow: 3px 3px 7px rgba(0,0,0,0.3);box-shadow: 3px 3px 7px rgba(0,0,0,0.3);\"><img decoding=\"async\" width=\"1200\" height=\"1008\" title=\"ZeissAxioObserver\" src=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2016\/11\/ZeissAxioObserver-e1504796162498.jpg\" alt class=\"img-responsive wp-image-1249\"\/><\/span><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_2 1_2 fusion-one-half fusion-column-last\" style=\"--awb-bg-size:cover;width:48%;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-1\"><h1>Axio Observer 7 Bright Field and Fluorescence Inverted Microscope with Peltier Controlled Thermal Stage<\/h1>\n<h3>Carl Zeiss<\/h3>\n<hr \/>\n<ul>\n<li>Peltier-controlled stage (4\u00b0C to 95\u00b0C) for isothermal and temperature gradient experiments<\/li>\n<li>Adaptive \u201cDefinite Focus 2\u201d stabilization<\/li>\n<li>Differential interference contrast (DIC)<\/li>\n<li>Camera Axiocam 702 mono CMOS camera with &#8220;Burst Mode&#8221; function<\/li>\n<li>Objectives including 5, 10, 20, 40, 40, 63 long working distance and 63X oil<\/li>\n<li>Colibri 7 light source containing 7 solid state LED lamps for fast excitation wavelength switching allowing for a wide range of dye analysis (red, yellow, green, cyan, blue, violet, UV)<\/li>\n<li>Fast filter wheel switching including DAPI, TRITC, CFP, GFP, mCherry, DsRED, Texas Red, Fura-2 calcium imaging, Cy3\/Cy5 FRET and CFP\/YFP FRET and similar emission wavelengths<\/li>\n<li>Hypoxia chamber with humidity, temperature controls and CO<sub>2<\/sub> and O<sub>2<\/sub> module<\/li>\n<li>Particle tracking analysis and optical sectioning deconvolution software<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-1 fusion-button-default-span fusion-button-default-type fusion-animated\" style=\"--button_accent_color:#ffffff;--button_accent_hover_color:#64a0c8;--button_border_hover_color:#64a0c8;--button_border_width-top:1px;--button_border_width-right:1px;--button_border_width-bottom:1px;--button_border_width-left:1px;--button_gradient_top_color:#000000;--button_gradient_bottom_color:#000000;--button_gradient_top_color_hover:#ffffff;--button_gradient_bottom_color_hover:#ffffff;\" data-animationType=\"fadeInDown\" data-animationDuration=\"0.1\" data-animationOffset=\"top-into-view\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.zeiss.com\/microscopy\/int\/products\/light-microscopes.html\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Learn more about the Axio Observer 7<\/span><\/a><\/div>\n<div class=\"fusion-alignleft\"><a class=\"fusion-button button-flat fusion-button-default-size button-custom fusion-button-default button-2 fusion-button-default-span fusion-button-default-type fusion-animated\" 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class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":116,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-180","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Bright Field and Fluorescence Microscope with Thermal Stage - The SBC Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/microscope\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Bright Field and Fluorescence 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