{"id":1369,"date":"2017-12-07T16:56:39","date_gmt":"2017-12-07T16:56:39","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/sbc-facility\/?page_id=1369"},"modified":"2024-12-13T15:11:59","modified_gmt":"2024-12-13T15:11:59","slug":"instrument-application-notes","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/sbc-facility\/instrument-application-notes\/","title":{"rendered":"Instrument Application Notes"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_2_3 2_3 fusion-two-third fusion-column-first\" style=\"--awb-bg-size:cover;width:66.666666666667%;width:calc(66.666666666667% - ( ( 4% ) * 0.66666666666667 ) );margin-right: 4%;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-1\"><h1>Instrument Application Notes<\/h1>\n<\/div><div class=\"fusion-text fusion-text-2\"><\/div><div class=\"fusion-text fusion-text-3\"><h2><strong>Bio-Inert HPLC:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-4\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2017\/12\/Bio-HPLC-Application-Note_ATP-ADP-Quantitation-Assay.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Bio-HPLC Application Note:\u00a0 ATP \/ ADP Quantitation Assay &#8211; Kinase \/ NTPase Assay (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2019\/07\/Bio-HPLC-MALS-QELS-RI-Application-Note_BSA-Molecular-Weight-Determination.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Bio-HPLC-MALS-QELS-RI Application Note:\u00a0 BSA Molecular Weight Determination (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-5\"><\/div><div class=\"fusion-text fusion-text-6\"><h2><strong>Calypso II (CG-MALS):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-7\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/02\/Calypso-CG-MALS-Application-Note_Protein-Protein-Interaction-Characterization.pdf\" target=\"_blank\" rel=\"noopener\">Calypso II CG-MALS Application Note:\u00a0 Protein-Protein Interaction Characterization (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-8\"><h2><strong>Circular Dichroism:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-9\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/01\/CD_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Circular Dichroism Spectrophotometer Application Note:\u00a0 General Introduction and Applications (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-10\"><\/div><div class=\"fusion-text fusion-text-11\"><h2><strong>Dynamic and Static Light Scattering (DLS \/ SLS):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-12\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2021\/02\/DynaPro-Plate-Reader_DLS_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">DynaPro DLS \/ SLS Plate Reader Application Note:\u00a0 General Introduction and Applications (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2021\/02\/DynaPro-Plate-Reader-Application-Note_DLS_SLS_kD-A2-MW.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">DynaPro DLS \/ SLS Plate Reader Application Note:\u00a0 kD, A2 \/ B22 and Molecular Weight Determination (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2021\/05\/DynaPro-Plate-Reader-Application-Note_Protein-Ligand-Interaction.pdf\" target=\"_blank\" rel=\"noopener\">DynaPro DLS \/ SLS Plate Reader Application Note:\u00a0 Protein \/ Ligand Interaction Screening and Characterization (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/07\/DynaPro-Plate-Reader-Application-Note_Thermal-and-Colloidal-Stability.pdf\" target=\"_blank\" rel=\"noopener\">DynaPro DLS \/ SLS Plate Reader Application Note:\u00a0 Thermal and Colloidal Stability (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-13\"><\/div><div class=\"fusion-text fusion-text-14\"><h2><strong>Differential Scanning Calorimetry (DSC):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-15\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2018\/01\/NanoDSC_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Nano DSC Application Note:\u00a0 General Introduction and Applications (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-16\"><\/div><div class=\"fusion-text fusion-text-17\"><h2><strong>Isothermal Titration Calorimetry (ITC):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-18\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/08\/PEAQ-ITC-Automated-General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener\">Automated PEAQ-ITC Application Note:\u00a0 General Introduction and Applications (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-19\"><\/div><div class=\"fusion-text fusion-text-20\"><h2><strong>Microscale Thermophoresis (MST):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-21\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2021\/03\/Monolith_MST_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Monolith NT.Automated Microscale Thermophoresis (MST) Application Note:\u00a0 General Introduction and Applications (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-22\"><\/div><div class=\"fusion-text fusion-text-23\"><h2><strong>Microscopy:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-24\"><h3><\/h3>\n<ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2020\/06\/Axio-Observer_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Axio Observer Fluorescence and Bright Field Microscope with Thermal Stage:\u00a0 General Introduction and Applications (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-25\"><\/div><div class=\"fusion-text fusion-text-26\"><h2><strong>Multi-Mode Assay Plate Reader:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-27\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2021\/02\/Neo2-Plate-Reader-Applicaion-Note_AlphaScreen.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Synergy Neo2 Multi-Mode Plate Reader Application Note:\u00a0 AlphaScreen, AlphaLisa &amp; AlphaPlex Assays (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2018\/01\/Multi-Mode-Plate-Reader-Application-Note_Bradford-Assay-Development.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">SpectraMax i3x Multi-Mode Plate Reader Application Note:\u00a0 Instrument and Method Validation for Protein Quantification using the Bradford Assay (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-28\"><h2><strong>MultiPep 2 Automated Peptide Synthesizer:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-29\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/02\/MultiPep2-Application-Note_Membrane-Spot-Peptide-Synthesis.pdf\" target=\"_blank\" rel=\"noopener\">MultiPep2 Automated Peptide Synthesizer Application Note:\u00a0 Membrane Spot Peptide Synthesis (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/02\/MultiPep2-Application-Note_CelluSpot-Slide-Spot-Peptide-Synthesis.pdf\" target=\"_blank\" rel=\"noopener\">MultiPep2 Automated Peptide Synthesizer Application Note:\u00a0 CelluSpot Slide Spot Peptide Synthesis (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-30\"><\/div><div class=\"fusion-text fusion-text-31\"><h2><strong>NanoSight:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-32\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2018\/01\/NanoSight-NTA_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">NanoSight NS300 NTA Application Note:\u00a0 General Introduction and Applications (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-33\"><\/div><div class=\"fusion-text fusion-text-34\"><h2><strong>Octet Biolayer Inferometry (BLI):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-35\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/12\/Octet-RED96-BLI_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener\">Octet RED96 BLI Application Note:\u00a0 General Introduction and Application Note (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-36\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/02\/Octet-RH16-BLI_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener\">Octet RH16 BLI Application Note:\u00a0 General Introduction and Application Note (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-37\"><\/div><div class=\"fusion-text fusion-text-38\"><h2><strong>Rheology (DHR-3):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-39\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/07\/Discovery-HR-3-Rheometer_General-Introduction-and-Application-Note.pdf\" target=\"_blank\" rel=\"noopener\">Discovery HR-3 Rheometer Application Note:\u00a0 General Introduction and Application Note (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-40\"><\/div><div class=\"fusion-text fusion-text-41\"><h2><strong>SAXSpace:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-42\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/07\/SAXSpace-Application-Note_Protein-Characterization.pdf\" target=\"_blank\" rel=\"noopener\">SAXSpace Application Note:\u00a0 Protein Characterization (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-43\"><\/div><div class=\"fusion-text fusion-text-44\"><h2><strong>StarGazer-2 Thermo-Denaturation:<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-45\"><h2><\/h2>\n<ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/01\/StarGazer-2-Application-Note-1_Protein-Optimal-Buffer-Screening.pdf\" target=\"_blank\" rel=\"noopener\">StarGazer-2 Application Note:\u00a0 Protein Optimal Buffer Screening (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/01\/StarGazer-2-Application-Note-2_Protein-Compound-Binding.pdf\" target=\"_blank\" rel=\"noopener\">StarGazer-2 Application Note:\u00a0 Protein-Compound Binding (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/01\/StarGazer-2-Application-Note-3_Protein-DNA-Mapping.pdf\" target=\"_blank\" rel=\"noopener\">StarGazer-2 Application Note:\u00a0 Protein-DNA Mapping (PDF)<\/a><\/h3>\n<\/li>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2023\/01\/StarGazer-2-Application-Note-4_Protein-Refolding-Condition-Screening.pdf\" target=\"_blank\" rel=\"noopener\">StarGazer-2 Application Note:\u00a0 Protein Refolding Condition Screening (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-text fusion-text-46\"><\/div><div class=\"fusion-text fusion-text-47\"><h2><strong>UV-VIS Spectrophotometry (Cary 3500):<\/strong><\/h2>\n<\/div><div class=\"fusion-text fusion-text-48\"><ul>\n<li>\n<h3><a href=\"https:\/\/lab.research.sickkids.ca\/sbc-facility\/wp-content\/uploads\/sites\/40\/2024\/07\/Cary-3500-UV-VIS-Spectrophotometer-Application-Note_Thermal-Melt-Analysis-of-Oligonucleotides.pdf\" target=\"_blank\" rel=\"noopener\">Cary 3500 UV-VIS Spectrophotometer Application Note:\u00a0 Thermal Melt Analysis of Oligonucleotides (PDF)<\/a><\/h3>\n<\/li>\n<\/ul>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_3 1_3 fusion-one-third fusion-column-last\" style=\"--awb-bg-size:cover;width:33.333333333333%;width:calc(33.333333333333% - ( ( 4% ) * 0.33333333333333 ) );\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":116,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1369","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Instrument Application Notes - The SBC Facility<\/title>\n<meta name=\"robots\" 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