{"id":228,"date":"2017-05-09T13:02:18","date_gmt":"2017-05-09T13:02:18","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/rosenblum\/?page_id=228"},"modified":"2019-08-27T19:07:09","modified_gmt":"2019-08-27T19:07:09","slug":"gallery","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/rosenblum\/gallery\/","title":{"rendered":"Gallery"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;]<section class=\"shortcode-teaser frame-on fadeIn animate-element shortcode-single-video\"><div class=\"shortcode-teaser-img\"><div class=\"shortcode-teaser-video\"><iframe loading=\"lazy\" width=\"1200\" height=\"675\" src=\"https:\/\/www.youtube.com\/embed\/zfF-1xIn_UE?feature=oembed\" frameborder=\"0\" allowfullscreen><\/iframe><\/div><\/div><div class=\"shortcode-teaser-content text-big\"><p>Ureteric outgrowth imaged in urogenital culture<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element shortcode-single-video\"><div class=\"shortcode-teaser-img\"><div class=\"shortcode-teaser-video\"><iframe loading=\"lazy\" width=\"1200\" height=\"675\" src=\"https:\/\/www.youtube.com\/embed\/gdIcB7pp9pk?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/div><\/div><div class=\"shortcode-teaser-content text-big\"><p>Embryonic mouse kidney explant cultured for 48 hours, then stained for Cytokeratin to mark ureteric branches. Imaged using Lightsheet Microscopy.<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/WT-CR2-Endo-Six2-10x_c1c2c3.jpg\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 450 363&#039;%2F%3E\" width=\"450\" height=\"363\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/WT-CR2-Endo-Six2-10x_c1c2c3.jpg 450w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/WT-CR2-Endo-Six2-10x_c1c2c3.jpg\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Mouse kidney stained with antibodies against Endomucin (vasculature) and SIX2 (nephrogenic cells)<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/ureter.jpg\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 450 337&#039;%2F%3E\" width=\"450\" height=\"337\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/ureter.jpg 450w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/ureter.jpg\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Mouse ureter stained with antibodies against smooth muscle actin and urinary pacemaker cells<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/YK05252017-3-10X-A_DAPILTLENCMCALB.png\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 4164 3120&#039;%2F%3E\" width=\"4164\" height=\"3120\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/YK05252017-3-10X-A_DAPILTLENCMCALB.png 4164w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/YK05252017-3-10X-A_DAPILTLENCMCALB.png\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Kidney stained for LTL (green) and Endomucin (red)<\/p>\n<\/div><\/section>[\/vc_column][vc_column width=&#8221;1\/3&#8243;]<section class=\"shortcode-teaser frame-on fadeIn animate-element shortcode-single-video\"><div class=\"shortcode-teaser-img\"><div class=\"shortcode-teaser-video\"><iframe loading=\"lazy\" width=\"1200\" height=\"900\" src=\"https:\/\/www.youtube.com\/embed\/5I5yHq2EzyE?feature=oembed\" frameborder=\"0\" allowfullscreen><\/iframe><\/div><\/div><div class=\"shortcode-teaser-content text-big\"><p><em>Ex vivo<\/em> assay demonstrating pyeloureteric contractions<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/YK07102017-E15.5-2-10X-A_Sall1VimentinDAPI-e1566414717877.png\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 4164 3120&#039;%2F%3E\" width=\"4164\" height=\"3120\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/YK07102017-E15.5-2-10X-A_Sall1VimentinDAPI-e1566414717877.png 4164w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/YK07102017-E15.5-2-10X-A_Sall1VimentinDAPI-e1566414717877.png\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Kidney stained for Vimentin (green) and Sall1 (red)<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/SIX2_WT1_explants.jpg\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 450 450&#039;%2F%3E\" width=\"450\" height=\"450\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/SIX2_WT1_explants.jpg 450w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/SIX2_WT1_explants.jpg\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Embryonic kidney explant stained with antibodies against WT1 (red) and SIX2 (green)<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/141104-WT2-20X-1_DAPISall1Pbx1.jpg\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 450 337&#039;%2F%3E\" width=\"450\" height=\"337\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/141104-WT2-20X-1_DAPISall1Pbx1.jpg 450w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/141104-WT2-20X-1_DAPISall1Pbx1.jpg\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Kidney stained with antibodies against Sall1 (green) and PBX1 (pink)<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/VIS-Wt1-LTL-ECAD-A_Alexa-405Alexa-488Alexa-568.jpg\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 969 1030&#039;%2F%3E\" width=\"969\" height=\"1030\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/VIS-Wt1-LTL-ECAD-A_Alexa-405Alexa-488Alexa-568.jpg 969w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/VIS-Wt1-LTL-ECAD-A_Alexa-405Alexa-488Alexa-568.jpg\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Kidney organoid section stained for WT1 (blue), LTL (green) and ECAD (red)<\/p>\n<\/div><\/section>[\/vc_column][vc_column width=&#8221;1\/3&#8243;]<section class=\"shortcode-teaser frame-on fadeIn animate-element shortcode-single-video\"><div class=\"shortcode-teaser-img\"><div class=\"shortcode-teaser-video\"><iframe loading=\"lazy\" width=\"1200\" height=\"675\" src=\"https:\/\/www.youtube.com\/embed\/O7hzjWIv84s?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/div><\/div><div class=\"shortcode-teaser-content text-big\"><p>Kidney organoid stained with WT1 and E-cadherin antibodies. Organoid imaged using Lightsheet Microscopy.<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/Control-Organoid-WT1-ECAD-DAPI.jpeg\" title=\"Kidney organoid histology image with different colours\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 830 830&#039;%2F%3E\" width=\"830\" height=\"830\" alt=\"Kidney organoid histology image with different colours\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/Control-Organoid-WT1-ECAD-DAPI.jpeg 830w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/Control-Organoid-WT1-ECAD-DAPI.jpeg\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Kidney organoid stained for WT1, E-cadherin and DAPI<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/e13.5-ctrl-1-10x_c1c2c3.png\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 4164 3120&#039;%2F%3E\" width=\"4164\" height=\"3120\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/e13.5-ctrl-1-10x_c1c2c3.png 4164w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/e13.5-ctrl-1-10x_c1c2c3.png\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>E13.5 kidney stained with SIX2 (green) and NCAM (red)<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/for-lab-website-e1566416134216.jpg\" title=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 450 450&#039;%2F%3E\" width=\"450\" height=\"450\" alt=\"Different coloured spots\/circles\/structures representing cells in a kidney histology image\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/for-lab-website-e1566416134216.jpg 450w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2017\/05\/for-lab-website-e1566416134216.jpg\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>Kidney explant stained with antibodies against E-cadherin (green) and WT1 (red)<\/p>\n<\/div><\/section><section class=\"shortcode-teaser frame-on fadeIn animate-element\"><div class=\"shortcode-teaser-img\"><a class=\"layzr-bg dt-pswp-item\" href=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/E13-Control-Cytokeratin-Sox9-highres.png\" title=\"Kidney wholemount image showing branches in green colour\" data-dt-img-description=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"lazy-load\" src=\"data:image\/svg+xml;charset=utf-8,%3Csvg xmlns%3D&#039;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#039; viewBox%3D&#039;0 0 1920 1080&#039;%2F%3E\" width=\"1920\" height=\"1080\" alt=\"Kidney wholemount image showing branches in green colour\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/E13-Control-Cytokeratin-Sox9-highres.png 1920w\" data-src=\"https:\/\/lab.research.sickkids.ca\/rosenblum\/wp-content\/uploads\/sites\/51\/2019\/08\/E13-Control-Cytokeratin-Sox9-highres.png\" \/><\/a><\/div><div class=\"shortcode-teaser-content text-big\"><p>E13.5 kidney stained for SOX9 (branches)<\/p>\n<\/div><\/section>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;][\/vc_column][vc_column 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