{"id":97,"date":"2015-10-27T15:50:45","date_gmt":"2015-10-27T15:50:45","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/palaniyar\/?page_id=97"},"modified":"2021-08-28T01:21:50","modified_gmt":"2021-08-28T01:21:50","slug":"net-research","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/","title":{"rendered":"NET Research"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;]<style type=\"text\/css\" data-type=\"the7_shortcodes-inline-css\">.shortcode-single-image-wrap.shortcode-single-image-e969c63221f3cf222940e87413ba1024.enable-bg-rollover .rollover i,\n.shortcode-single-image-wrap.shortcode-single-image-e969c63221f3cf222940e87413ba1024.enable-bg-rollover .rollover-video i {\n  background: -webkit-linear-gradient();\n  background: linear-gradient();\n}\n.shortcode-single-image-wrap.shortcode-single-image-e969c63221f3cf222940e87413ba1024 .rollover-icon {\n  font-size: 32px;\n  color: #ffffff;\n  min-width: 44px;\n  min-height: 44px;\n  line-height: 44px;\n  border-radius: 100px;\n  border-style: solid;\n  border-width: 0px;\n}\n.dt-icon-bg-on.shortcode-single-image-wrap.shortcode-single-image-e969c63221f3cf222940e87413ba1024 .rollover-icon {\n  background: rgba(255,255,255,0.3);\n  box-shadow: none;\n}<\/style><div class=\"shortcode-single-image-wrap shortcode-single-image-e969c63221f3cf222940e87413ba1024 alignnone  enable-bg-rollover dt-icon-bg-off\" style=\"margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; width:500px;\"><div class=\"shortcode-single-image\"><div class=\"fancy-media-wrap  layzr-bg\" style=\"\"><img fetchpriority=\"high\" decoding=\"async\" class=\"preload-me lazy-load aspect\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D&#39;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#39;%20viewBox%3D&#39;0%200%20500%20423&#39;%2F%3E\" data-src=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/01\/P.a_SEM-500x423.jpg\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/01\/P.a_SEM-500x423.jpg 500w, https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/01\/P.a_SEM.jpg 607w\" loading=\"eager\" sizes=\"(max-width: 500px) 100vw, 500px\" width=\"500\" height=\"423\"  data-dt-location=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/p-a_sem\/\" style=\"--ratio: 500 \/ 423;\" alt=\"\" \/><\/div><\/div><\/div>[vc_empty_space height=&#8221;40px&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h1><strong>Neutrophil Extracellular Traps<\/strong><\/h1>\n<div style=\"text-align: left\">Neutrophils are immune cells that are very important in defending your body against infections and foreign particles. One of their function is to cast NETs (<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31416173\/\">Neutrophil Extracellular Traps<\/a>), a network of their own DNA strands, to trap bacteria and other pathogens. NETs are useful for trapping pathogens during acute infections, preventing the spread of pathogens into other tissues and killing ensnared pathogens. However, excess NETs released into the tissues can severely damage organs, particularly in chronic inflammatory conditions and chronic diseases. NETs also contribute to autoantibody generation that can facilitate the development of autoimmune diseases. We aim to understand the molecular mechanisms governing NETosis, and to identify drugs that regulate NET formation. We expect that our work will help to control inflammatory and autoimmune diseases.<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h1><strong>NET Formation Pathways<\/strong><\/h1>\n<p>NETosis or NET formation can occur via 2 different pathways depending on the agonist used, and are differentiated by whether certain enzymes, specifically NADPH Oxidase 2 (NOX2) are used.<\/p>\n<p>Lipopolysaccharide (LPS), a component of gram-negative bacteria, and Phorbol 12-myristate 13-acetate (PMA), a synthetic compound, are stimulators of the NOX-dependent Pathway. These agonists will stimulate NOX2 to produce large amounts of reactive oxidative species (ROS) which contain free radicals (The stuff that antioxidants neutralize).<\/p>\n<p>Whereas the influx of calcium ions into the neutrophils, whether caused by the administration of drugs (Ex. calcium ionophores) or activation of transporter proteins (Ex. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25730848\/\">SK3 channels<\/a>) promote the NOX-independent pathway to produce NETs faster than the other pathway. The influx of calcium ions into the neutrophil will encourage the mitochondria to produce ROS. These calcium ions also bind with PAD4, initiating histone citrullination, a process that facilitates chromatin decondensation which unravels the DNA within the cell nucleus.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<style type=\"text\/css\" data-type=\"the7_shortcodes-inline-css\">.shortcode-single-image-wrap.shortcode-single-image-ddafd708c23223534f51709c3abb5042.enable-bg-rollover .rollover i,\n.shortcode-single-image-wrap.shortcode-single-image-ddafd708c23223534f51709c3abb5042.enable-bg-rollover .rollover-video i {\n  background: -webkit-linear-gradient();\n  background: linear-gradient();\n}\n.shortcode-single-image-wrap.shortcode-single-image-ddafd708c23223534f51709c3abb5042 .rollover-icon {\n  font-size: 32px;\n  color: #ffffff;\n  min-width: 44px;\n  min-height: 44px;\n  line-height: 44px;\n  border-radius: 100px;\n  border-style: solid;\n  border-width: 0px;\n}\n.dt-icon-bg-on.shortcode-single-image-wrap.shortcode-single-image-ddafd708c23223534f51709c3abb5042 .rollover-icon {\n  background: rgba(255,255,255,0.3);\n  box-shadow: none;\n}<\/style><div class=\"shortcode-single-image-wrap shortcode-single-image-ddafd708c23223534f51709c3abb5042 alignnone  enable-bg-rollover dt-icon-bg-off\" style=\"margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; width:500px;\"><div class=\"shortcode-single-image\"><div class=\"fancy-media-wrap\" style=\"\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5296899\/\" class=\" layzr-bg rollover\" style=\"\" aria-label=\"Image\"><img decoding=\"async\" class=\"preload-me lazy-load aspect\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D&#39;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#39;%20viewBox%3D&#39;0%200%20500%20579&#39;%2F%3E\" data-src=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/07\/NETosis-Overall-1-500x579.jpg\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/07\/NETosis-Overall-1-500x579.jpg 500w, https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/07\/NETosis-Overall-1.jpg 787w\" loading=\"eager\" style=\"--ratio: 500 \/ 579\" sizes=\"(max-width: 500px) 100vw, 500px\" width=\"500\" height=\"579\"  data-dt-location=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/netosis-overall\/\" alt=\"\" \/><\/a><\/div><\/div><\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]Both of these ROS will activate different sets of kinases, and disintegrate the membranes of the nucleus and granules. The disintegration of the membranes will allow proteins, granules, and enzymes such as Myeloperoxidase and Neutrophil Elastase to interact with and unravel the DNA (to cleave the histones, or facilitate chromatin decondensation). These decondensed DNA at this point are decorated with granular contents and are released out of the neutrophils as NETs.[\/vc_column_text][vc_column_text]An important discovery of our lab was the role of <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28176807\/\">transcriptional firing in NETosis<\/a>. We found that NETosis-specific kinase cascades activate transcription factors, starting transcription at multiple loci at all chromosomes to facilitate chromatin decondensation (An important step in creating RNA and later on, proteins). When we inhibit transcription, both the NOX-dependent and NOX-independent pathways were suppressed. However, inhibition of translation (Process that makes proteins) did not affect NETosis.[\/vc_column_text][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;]<style type=\"text\/css\" data-type=\"the7_shortcodes-inline-css\">.shortcode-single-image-wrap.shortcode-single-image-656a8ec4e2525e6c7d09529d64277c38.enable-bg-rollover .rollover i,\n.shortcode-single-image-wrap.shortcode-single-image-656a8ec4e2525e6c7d09529d64277c38.enable-bg-rollover .rollover-video i {\n  background: -webkit-linear-gradient();\n  background: linear-gradient();\n}\n.shortcode-single-image-wrap.shortcode-single-image-656a8ec4e2525e6c7d09529d64277c38 .rollover-icon {\n  font-size: 32px;\n  color: #ffffff;\n  min-width: 44px;\n  min-height: 44px;\n  line-height: 44px;\n  border-radius: 100px;\n  border-style: solid;\n  border-width: 0px;\n}\n.dt-icon-bg-on.shortcode-single-image-wrap.shortcode-single-image-656a8ec4e2525e6c7d09529d64277c38 .rollover-icon {\n  background: rgba(255,255,255,0.3);\n  box-shadow: none;\n}<\/style><div class=\"shortcode-single-image-wrap shortcode-single-image-656a8ec4e2525e6c7d09529d64277c38 alignnone  enable-bg-rollover dt-icon-bg-off\" style=\"margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; width:500px;\"><div class=\"shortcode-single-image\"><div class=\"fancy-media-wrap\" style=\"\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8128883\/\" class=\" layzr-bg rollover\" style=\"\" aria-label=\"Image\"><img decoding=\"async\" class=\"preload-me lazy-load aspect\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D&#39;http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg&#39;%20viewBox%3D&#39;0%200%20500%20606&#39;%2F%3E\" data-src=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/07\/ROS-Induces-DNA-Repair-in-NETosis-1-500x606.jpg\" data-srcset=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/07\/ROS-Induces-DNA-Repair-in-NETosis-1-500x606.jpg 500w, https:\/\/lab.research.sickkids.ca\/palaniyar\/wp-content\/uploads\/sites\/19\/2021\/07\/ROS-Induces-DNA-Repair-in-NETosis-1-673x815.jpg 673w\" loading=\"eager\" style=\"--ratio: 500 \/ 606\" sizes=\"(max-width: 500px) 100vw, 500px\" width=\"500\" height=\"606\"  data-dt-location=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/ros-induces-dna-repair-in-netosis-2\/\" alt=\"\" \/><\/a><\/div><\/div><\/div>[\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1630113676398{padding-top: 80px !important;padding-bottom: 80px !important;}&#8221;]<\/p>\n<h1><strong>ROS Induces DNA Repair in NETosis<\/strong><\/h1>\n<p>A new and exciting discovery! We discovered that <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34001856\/\">ROS induces NETosis by initiating DNA repair<\/a>. When ROS interacts with the chromatin in the nucleus, they perform extensive DNA damage, oxidizing the guanine residues in the DNA, creating 8-Oxoguanine (Markers of DNA lesions). When transcription occurs, such as when NETosis causes genome-wide transcription, the transcription machinery will stall at the sites of DNA lesions and signal other proteins to initiate DNA repair.<\/p>\n<p>The DNA repair machinery assemble at the sites of DNA damage, and in the process of repairing, decondenses the chromatin. The DNA-unwinding capabilities of these DNA repair proteins are key drivers of NETosis.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][vc_column_text]<\/p>\n<hr \/>\n<p>[\/vc_column_text][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column]<div id=\"ultimate-heading-672769efacaa00c59\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-672769efacaa00c59 uvc-6761 \" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-672769efacaa00c59 h2'  data-responsive-json-new='{\"font-size\":\"desktop:22px;\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Frequently Used Techniques<\/h2><\/div><\/div>[vc_empty_space height=&#8221;10px&#8221;][vc_column_text]Below is a diagram outlining techniques we use regularly in our experiments regarding NETs.\u00a0This work can be done with either HL-60 cells (<em>in vivo<\/em>) that can be differentiated into neutrophil-like cells using DMSO, or primary neutrophils obtained from volunteers (<em>ex vivo)<\/em>.[\/vc_column_text][vc_single_image image=&#8221;331&#8243; img_size=&#8221;full&#8221;][vc_empty_space height=&#8221;10px&#8221;][vc_column_text]Once the cells are properly isolated, they will be stimulated by a variety of agonists. These include: PMA, A23187, ionomycin, LPS, <i>P. a, S. a<\/i>, PAF, Pyocyanin, IC, and HxA3. After they are stimulated, the cells will follow two different paths. They can be pipetted into 96-well plates for to be imaged, to have their ROS levels measured, or have cell viability measured among other things. They could also be left in the microtube so that other aspects of the cells can be measured such as protein production, and gene transcription. Possible methods\u00a0are shown in the image below.[\/vc_column_text][vc_single_image image=&#8221;334&#8243; img_size=&#8221;full&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space height=&#8221;40px&#8221;]<div id=\"ultimate-heading-271769efacaa00c91\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-271769efacaa00c91 uvc-7148 \" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-271769efacaa00c91 h2'  data-responsive-json-new='{\"font-size\":\"desktop:22px;\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Experimental models<\/h2><\/div><\/div>[vc_empty_space height=&#8221;10px&#8221;][vc_column_text]<\/p>\n<p style=\"text-align: left\">Below are diagrams that depict the various experimental models used in our experiments. The majority of our models are in mice, though we do use a single horse model. The diagrams show the type of model, briefly how they are created, data depicting their use, and publications in which they are utilized.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;]<div id=\"ultimate-heading-428669efacaa00cb3\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-428669efacaa00cb3 uvc-4966  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-428669efacaa00cb3 h2'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Mouse<\/h2><\/div><\/div>[vc_single_image image=&#8221;664&#8243; img_size=&#8221;large&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; image_hovers=&#8221;false&#8221; link=&#8221;http:\/\/www.jimmunol.org\/content\/early\/2011\/07\/01\/jimmunol.1004201.full.pdf&#8221;][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div id=\"ultimate-heading-654469efacaa00cd2\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-654469efacaa00cd2 uvc-2252  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-654469efacaa00cd2 h2'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Mouse<\/h2><\/div><\/div>[vc_single_image image=&#8221;673&#8243; img_size=&#8221;large&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; image_hovers=&#8221;false&#8221; link=&#8221;http:\/\/www.jimmunol.org\/content\/early\/2011\/07\/01\/jimmunol.1004201.full.pdf&#8221;][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div id=\"ultimate-heading-353069efacaa00cee\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-353069efacaa00cee uvc-5165  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-353069efacaa00cee h2'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Mouse<\/h2><\/div><\/div>[vc_single_image image=&#8221;666&#8243; img_size=&#8221;large&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; image_hovers=&#8221;false&#8221; link=&#8221;http:\/\/anesthesiology.pubs.asahq.org\/article.aspx?articleid=2205935&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][vc_column_text]<\/p>\n<hr \/>\n<p>[\/vc_column_text][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;]<div id=\"ultimate-heading-617369efacaa00d0a\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-617369efacaa00d0a uvc-1967  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-617369efacaa00d0a h2'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Mouse<\/h2><\/div><\/div>[vc_single_image image=&#8221;674&#8243; img_size=&#8221;large&#8221; alignment=&#8221;center&#8221; image_hovers=&#8221;false&#8221;][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div id=\"ultimate-heading-196469efacaa00d26\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-196469efacaa00d26 uvc-3565  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-196469efacaa00d26 h2'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Mouse<\/h2><\/div><\/div>[vc_single_image image=&#8221;672&#8243; img_size=&#8221;large&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; image_hovers=&#8221;false&#8221; link=&#8221;http:\/\/www.jimmunol.org\/content\/193\/7\/3549.long&#8221;][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div id=\"ultimate-heading-595169efacaa00d42\" class=\"uvc-heading ult-adjust-bottom-margin ultimate-heading-595169efacaa00d42 uvc-2461  uvc-heading-default-font-sizes\" data-hspacer=\"no_spacer\"  data-halign=\"center\" style=\"text-align:center\"><div class=\"uvc-heading-spacer no_spacer\" style=\"top\"><\/div><div class=\"uvc-main-heading ult-responsive\"  data-ultimate-target='.uvc-heading.ultimate-heading-595169efacaa00d42 h2'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}' ><h2 style=\"--font-weight:theme;\">Horse<\/h2><\/div><\/div>[vc_single_image image=&#8221;663&#8243; img_size=&#8221;large&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; image_hovers=&#8221;false&#8221; link=&#8221;http:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0096217&#8243;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_empty_space height=&#8221;40px&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text] Neutrophil Extracellular Traps Neutrophils are immune cells that are very important in defending your body against infections and foreign particles. One of their function is to cast NETs (Neutrophil Extracellular Traps), a network of their own DNA strands, to trap bacteria and other pathogens. NETs are useful for trapping pathogens during&hellip;<\/p>\n","protected":false},"author":57,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-97","page","type-page","status-publish","hentry","description-off"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>NET Research - Palaniyar Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NET Research\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_empty_space height=&#8221;40px&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text] Neutrophil Extracellular Traps Neutrophils are immune cells that are very important in defending your body against infections and foreign particles. One of their function is to cast NETs (Neutrophil Extracellular Traps), a network of their own DNA strands, to trap bacteria and other pathogens. NETs are useful for trapping pathogens during&hellip;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/\" \/>\n<meta property=\"og:site_name\" content=\"Palaniyar Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2021-08-28T01:21:50+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"6 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/\",\"url\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/\",\"name\":\"NET Research - Palaniyar Lab\",\"isPartOf\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/#website\"},\"datePublished\":\"2015-10-27T15:50:45+00:00\",\"dateModified\":\"2021-08-28T01:21:50+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"NET Research\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/#website\",\"url\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/\",\"name\":\"Palaniyar Lab\",\"description\":\"Collectin NETs\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/lab.research.sickkids.ca\/palaniyar\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"NET Research - Palaniyar Lab","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/lab.research.sickkids.ca\/palaniyar\/net-research\/","og_locale":"en_US","og_type":"article","og_title":"NET Research","og_description":"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_empty_space height=&#8221;40px&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text] Neutrophil Extracellular Traps Neutrophils are immune cells that are very important in defending your body against infections and foreign particles. One of their function is to cast NETs (Neutrophil Extracellular Traps), a network of their own DNA strands, to trap bacteria and other pathogens. 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