{"id":10,"date":"2020-11-03T19:01:56","date_gmt":"2020-11-03T19:01:56","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/nbif\/?page_id=10"},"modified":"2025-07-23T18:19:28","modified_gmt":"2025-07-23T18:19:28","slug":"3d-sem","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/nbif\/3d-sem\/","title":{"rendered":"3D SEM"},"content":{"rendered":"<p><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container video-background hundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-no-small-visibility\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:100px;--awb-padding-right:40px;--awb-padding-bottom:100px;--awb-padding-left:40px;--awb-background-color:rgba(255,255,255,0);--awb-flex-wrap:wrap;\" ><div class=\"fullwidth-video-image\" style=\"background-image:url(https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/12\/nbif-hero-1024x534.png);\"><\/div><div class=\"fullwidth-video\"><video preload=\"auto\" autoplay playsinline loop muted><source src=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/12\/5.mp4\" type=\"video\/mp4\"><\/video><\/div><div class=\"fullwidth-overlay\" style=\"background-color:rgba(255,255,255,0);\"><\/div><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"width:104% !important;max-width:104% !important;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-2 fusion-flex-container hundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-no-small-visibility\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:20px;--awb-padding-right:40px;--awb-padding-left:40px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"width:104% !important;max-width:104% !important;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-1 sep-underline sep-solid fusion-title-center fusion-title-text fusion-title-size-one\" style=\"--awb-text-color:#69923a;--awb-sep-color:rgba(105,146,58,0.1);\"><h1 class=\"fusion-title-heading title-heading-center\" style=\"margin:0;\"><b>3D Scanning Electron Microscopy (3D SEM)<\/b><\/h1><\/div><div class=\"fusion-text fusion-text-1\"><p>The 3D SEM core provides cutting-edge scanning electron microscopy (SEM) equipment. We employ the latest generation of electron microscopes with advanced technology to support revolutionary cell biology research. We offer fast image acquisition, high resolving power and the ability to generate 3-dimensional images in a world-class laboratory.<\/p>\n<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-3 fusion-flex-container hundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-no-medium-visibility fusion-no-large-visibility\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-2 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:0px;--awb-margin-bottom-large:0px;--awb-spacing-left-large:0px;--awb-width-medium:100%;--awb-spacing-right-medium:0px;--awb-spacing-left-medium:0px;--awb-width-small:100%;--awb-spacing-right-small:0px;--awb-spacing-left-small:0px;\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-2 fusion-no-medium-visibility fusion-no-large-visibility\"><p style=\"text-align: center;font-size: 30px;line-height: 34.8px\"><span style=\"color: #69923a;font-size: 24px;font-family: Lato\" data-fusion-font=\"true\" data-fusion-google-font=\"Lato\" data-fusion-google-variant=\"400\">3D Scanning Electron Microscopy (3D SEM)<\/span><\/p>\n<\/div><div class=\"fusion-text fusion-text-3\"><p style=\"font-size: 16px;line-height: 28.8px\" data-fusion-font=\"true\">The 3D SEM core provides cutting-edge scanning electron microscopy (SEM) equipment. We employ the latest generation of electron microscopes with advanced technology to support revolutionary cell biology research. We offer fast image acquisition, high resolving power and the ability to generate 3-dimensional images in a world-class laboratory.<\/p>\n<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-4 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-right:0px;--awb-padding-left:0px;--awb-background-color:rgba(0,70,173,0.1);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1248px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-3 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-section-separator section-separator bigtriangle fusion-section-separator-1\" style=\"--awb-spacer-height:99px;--awb-svg-margin-left:1.92%;--awb-svg-margin-right:1.92%;--awb-svg-margin-left-medium:1.92%;--awb-svg-margin-right-medium:1.92%;--awb-svg-margin-left-small:1.92%;--awb-svg-margin-right-small:1.92%;--awb-divider-height:99px;--awb-spacer-padding-top:inherit;--awb-sep-padding:0;--awb-svg-padding:0;\"><div class=\"fusion-section-separator-svg\"><svg class=\"fusion-big-triangle-candy\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\" width=\"100%\" height=\"100\" viewBox=\"0 0 100 100\" preserveAspectRatio=\"none\" fill=\"rgba(255,255,255,1)\"><path d=\"M-1 -1 L50 99 L101 -1 Z\"><\/path><\/svg><\/div><div class=\"fusion-section-separator-spacer\"><div class=\"fusion-section-separator-spacer-height\"><\/div><\/div><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:40px;width:100%;\"><\/div><div class=\"fusion-menu-anchor\" id=\"equipment3dsem\"><\/div><div class=\"fusion-title title fusion-title-2 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-font-size:45px;\"><h2 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;font-size:1em;\"><b>Equipment<br \/>\n<\/b><\/h2><\/div><div class=\"fusion-title title fusion-title-3 fusion-sep-none fusion-title-text fusion-title-size-three\"><h3 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;\">Zeiss Gemini 2 SEM<\/h3><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"width:104% !important;max-width:104% !important;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-0 fusion_builder_column_inner_2_5 2_5 fusion-flex-column fusion-flex-align-self-flex-start\" style=\"--awb-bg-size:cover;--awb-width-large:40%;--awb-margin-top-large:0px;--awb-spacing-right-large:4.8%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:4.8%;--awb-width-medium:40%;--awb-order-medium:0;--awb-spacing-right-medium:4.8%;--awb-spacing-left-medium:4.8%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-center fusion-content-layout-column\"><div class=\"fusion-image-element \" style=\"--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-none imageframe-1 hover-type-none\"><a href=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-scaled.jpg\" class=\"fusion-lightbox\" data-rel=\"iLightbox[02caee1f50e8ddf44dc]\" data-title=\"zeiss-gemini\" title=\"zeiss-gemini\"><img decoding=\"async\" width=\"2560\" height=\"1920\" src=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-scaled.jpg\" alt class=\"img-responsive wp-image-55\" srcset=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-200x150.jpg 200w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-400x300.jpg 400w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-600x450.jpg 600w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-800x600.jpg 800w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-1200x900.jpg 1200w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-gemini-scaled.jpg 2560w\" sizes=\"(max-width: 640px) 100vw, 600px\" \/><\/a><\/span><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-1 fusion_builder_column_inner_3_5 3_5 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:60%;--awb-margin-top-large:0px;--awb-spacing-right-large:3.2%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:3.2%;--awb-width-medium:60%;--awb-order-medium:0;--awb-spacing-right-medium:3.2%;--awb-spacing-left-medium:3.2%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-4\"><p>The Gemini is a field emission gun microscope equipped with the Gatan 3View serial block face for Automate sectioning and image capture of 3D ultrastructures.<\/p>\n<p>It is capable of imaging at low voltages below 1kV with very small probe sizes and high signal-to-noise (SNR) ratio thanks to novel beam booster technology.<\/p>\n<p>There are five detectors available on this machine:<\/p>\n<ol>\n<li><span style=\"color: #000000\">Secondary electron detector<\/span><\/li>\n<li><span style=\"color: #000000\">Inlens SE detector<\/span><\/li>\n<li><span style=\"color: #000000\">Backscattered electron detector, four-segment annular design<\/span><\/li>\n<li><span style=\"color: #000000\">Inlens Energy selective backscattered electron detector<\/span><\/li>\n<li><span style=\"color: #000000\">OnPoint Gatan backscattered electron detector for block face imaging<\/span><\/li>\n<\/ol>\n<\/div><\/div><\/div><\/div><div class=\"fusion-title title fusion-title-4 fusion-sep-none fusion-title-text fusion-title-size-three\"><h3 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;\">Zeiss Crossbeam 550<\/h3><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"width:104% !important;max-width:104% !important;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-2 fusion_builder_column_inner_2_5 2_5 fusion-flex-column fusion-flex-align-self-stretch\" style=\"--awb-bg-size:cover;--awb-width-large:40%;--awb-margin-top-large:0px;--awb-spacing-right-large:4.8%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:4.8%;--awb-width-medium:40%;--awb-order-medium:0;--awb-spacing-right-medium:4.8%;--awb-spacing-left-medium:4.8%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-image-element \" style=\"--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-none imageframe-2 hover-type-none\"><a href=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-scaled.jpg\" class=\"fusion-lightbox\" data-rel=\"iLightbox[41a10213f958f038bae]\" data-title=\"zeiss-crossbeam\" title=\"zeiss-crossbeam\"><img decoding=\"async\" width=\"2560\" height=\"1920\" src=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-scaled.jpg\" alt class=\"img-responsive wp-image-54\" srcset=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-200x150.jpg 200w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-400x300.jpg 400w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-600x450.jpg 600w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-800x600.jpg 800w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-1200x900.jpg 1200w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/zeiss-crossbeam-scaled.jpg 2560w\" sizes=\"(max-width: 640px) 100vw, 600px\" \/><\/a><\/span><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-3 fusion_builder_column_inner_3_5 3_5 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:60%;--awb-margin-top-large:0px;--awb-spacing-right-large:3.2%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:3.2%;--awb-width-medium:60%;--awb-order-medium:0;--awb-spacing-right-medium:3.2%;--awb-spacing-left-medium:3.2%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-5\"><p data-fusion-font=\"true\">The Crossbeam is a field emission scanning electron microscope with the processing ability of a focused ion beam.<\/p>\n<p>This instrument uses a Gallium focused ion beam for serial milling of samples and sequential SEM imaging. This beam can remove sections as thin as 5 nm, providing the highest possible z-plane resolution of biological samples.<\/p>\n<p>There are six key features available on this machine:<\/p>\n<ol>\n<li>SESI detector for secondary electron and secondary ion imaging<\/li>\n<li>Inlens SE detector<\/li>\n<li>Backscattered electron detector, four-channel parallel annular design<\/li>\n<li>Inlens Energy selective backscattered electron detector<\/li>\n<li>Two channels Gas Injection System (GIS) for Platinum and Carbon deposition<\/li>\n<li>Annular STEM detector for transmission imaging<\/li>\n<\/ol>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-5 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:20px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-stretch fusion-flex-justify-content-space-between fusion-flex-content-wrap\" style=\"max-width:1248px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-4 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-menu-anchor\" id=\"services3dsem\"><\/div><div class=\"fusion-title title fusion-title-5 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-font-size:45px;\"><h2 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;font-size:1em;\"><b>Services<br \/>\n<\/b><\/h2><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-5 fusion_builder_column_1_3 1_3 fusion-flex-column fusion-flex-align-self-flex-start\" style=\"--awb-bg-size:cover;--awb-width-large:33.333333333333%;--awb-margin-top-large:0px;--awb-spacing-right-large:5.76%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:5.76%;--awb-width-medium:33.333333333333%;--awb-order-medium:0;--awb-spacing-right-medium:5.76%;--awb-spacing-left-medium:5.76%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-6\"><h3>Serial Block Face (SBF) imaging<\/h3>\n<\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-6 fusion_builder_column_2_3 2_3 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:66.666666666667%;--awb-margin-top-large:0px;--awb-spacing-right-large:2.88%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:2.88%;--awb-width-medium:66.666666666667%;--awb-order-medium:0;--awb-spacing-right-medium:2.88%;--awb-spacing-left-medium:2.88%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-7\"><p>3D SBF imaging involves the use of an ultramicrotome mounted inside the vacuum chamber of a SEM to cut a thin section from the face of the block. After the section is cut, the specimen is raised back to the focal plane and imaged again. This sequence of specimen imaging, section cutting and block raising can acquire thousands of images in perfect alignment in an automated fashion.<\/p>\n<\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-7 fusion_builder_column_1_3 1_3 fusion-flex-column fusion-flex-align-self-flex-start\" style=\"--awb-bg-size:cover;--awb-width-large:33.333333333333%;--awb-margin-top-large:0px;--awb-spacing-right-large:5.76%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:5.76%;--awb-width-medium:33.333333333333%;--awb-order-medium:0;--awb-spacing-right-medium:5.76%;--awb-spacing-left-medium:5.76%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-8\"><h3>Array Tomography and Correlative Light Electron Microscopy<\/h3>\n<\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-8 fusion_builder_column_2_3 2_3 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:66.666666666667%;--awb-margin-top-large:0px;--awb-spacing-right-large:2.88%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:2.88%;--awb-width-medium:66.666666666667%;--awb-order-medium:0;--awb-spacing-right-medium:2.88%;--awb-spacing-left-medium:2.88%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-9\"><p>It can be challenging to determine where specific molecules, like proteins or lipids, are located relative to cell structures. Correlative light-EM (CLEM) imaging is one method used to do this. CLEM merges the power of light microscopy (with its many fluorescentreporters of protein\/lipid location) with the resolving power of EM. The ATLAS 5 software includes dedicated software for semi-automated SEM imaging of serial sections at multiple resolutions for Array Tomography. ATLAS 5 software enables advanced, correlative microscopy by fusion of data sets from multiple instruments, detectors and sessions into one correlative workspace.<\/p>\n<\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-9 fusion_builder_column_1_3 1_3 fusion-flex-column fusion-flex-align-self-flex-start\" style=\"--awb-bg-size:cover;--awb-width-large:33.333333333333%;--awb-margin-top-large:0px;--awb-spacing-right-large:5.76%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:5.76%;--awb-width-medium:33.333333333333%;--awb-order-medium:0;--awb-spacing-right-medium:5.76%;--awb-spacing-left-medium:5.76%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-10\"><h3>FIB-SEM Imaging<\/h3>\n<\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-10 fusion_builder_column_2_3 2_3 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:66.666666666667%;--awb-margin-top-large:0px;--awb-spacing-right-large:2.88%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:2.88%;--awb-width-medium:66.666666666667%;--awb-order-medium:0;--awb-spacing-right-medium:2.88%;--awb-spacing-left-medium:2.88%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-11\"><p>Our facility can image at high-resolutions while milling with a focused ion beam. The Atlas 5 3-D nanotomography package allows for simultaneous milling and imaging without having to switch off either beam during the entire acquisition process. The resulting high z-resolution can visualize membrane alterations and their association with other cellular structures. We can employ high pressure freezing to provide ideal sample preservation and minimize potential imaging artefacts.<\/p>\n<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-6 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:20px;--awb-padding-bottom:29px;--awb-background-color:rgba(0,70,173,0.1);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1248px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-11 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-menu-anchor\" id=\"fees3dsem\"><\/div><div class=\"fusion-title title fusion-title-6 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-font-size:45px;\"><h2 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;font-size:1em;\"><p><b>Fees<br \/>\n<\/b><\/p><\/h2><\/div><div class=\"fusion-text fusion-text-12\"><p>The 3D SEM core provides high quality service, competitive prices, and a wide range of available techniques. We offer consultation, sample preparation, user training and data acquisition.<\/p>\n<\/div>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\">Service<\/th>\n<th align=\"left\">Internal cost<\/th>\n<th align=\"left\">External cost<\/th>\n<th align=\"left\">Industry cost<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">Gemini SEM<\/td>\n<td align=\"left\">$220\/day<\/td>\n<td align=\"left\">$220\/day<\/td>\n<td align=\"left\">$440\/day<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Crossbeam SEM<\/td>\n<td align=\"left\">$220\/day<\/td>\n<td align=\"left\">$220\/day<\/td>\n<td align=\"left\">$440\/day<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">Technologist&#8217;s time<\/td>\n<td align=\"left\">$82\/hour<\/td>\n<td align=\"left\">$82\/hour<\/td>\n<td align=\"left\">$160\/hour<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-7 fusion-flex-container has-pattern-background has-mask-background nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-margin-bottom:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1248px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-12 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:15px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-title title fusion-title-7 fusion-sep-none fusion-title-text fusion-title-size-two\"><h2 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;\"><strong>Contact and bookings<\/strong><\/h2><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-13 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-padding-top:25px;--awb-padding-right:25px;--awb-padding-bottom:25px;--awb-padding-left:25px;--awb-bg-color:rgba(0,70,173,0.1);--awb-bg-color-hover:rgba(0,70,173,0.1);--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-row\"><div class=\"fusion-text fusion-text-13\"><p>The Nanoscale Biomedical Imaging Facility (NBIF) is a core facility at The Hospital for Sick Children (SickKids) Research Institute. Please review the <a href=\"https:\/\/www.sickkids.ca\/siteassets\/research\/sickkids-core-facilities-terms-conditions.pdf\" target=\"_blank\" rel=\"noopener\">SickKids Core Facility Terms and Conditions (PDF)<\/a>\u00a0before submitting a service request.<\/p>\n<\/div><\/div><\/div>\n<div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-14 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-14\"><p>In order to access the 3D SEM services, new clients must submit an application form to describe their projects and approach. The facility manager and directors will consult the client on suitable imaging methods and sample preparation.<\/p>\n<p>Once the application has been approved, samples will be processed in the CMEM sub-core and screened for quality using our TEM.<\/p>\n<p>Successful samples will then be prepared for volume imaging and imaged at the 3D SEM facility.<\/p>\n<\/div><div ><a class=\"fusion-button button-flat button-xlarge button-default fusion-button-default button-1 fusion-button-default-span fusion-button-default-type\" style=\"--button-border-radius-top-left:50px;--button-border-radius-top-right:50px;--button-border-radius-bottom-right:50px;--button-border-radius-bottom-left:50px;\" target=\"_self\" href=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/3DSEM-Project-Request-Form.pdf\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Download the 3D SEM application form<\/span><i class=\"fa-angle-right fas awb-button__icon awb-button__icon--default button-icon-right\" aria-hidden=\"true\"><\/i><\/a><\/div><div class=\"fusion-text fusion-text-15\" style=\"--awb-margin-top:25px;\"><p>For questions or more information about our services, please contact Dr. Ali Darbandi at <a href=\"mailto:sb@sickkids.ca\"><u>ali.darbandi@sickkids.ca<\/u><\/a><\/p>\n<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-8 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:20px;--awb-background-color:rgba(0,70,173,0.1);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-end fusion-flex-justify-content-space-evenly fusion-flex-content-wrap\" style=\"max-width:1248px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-15 fusion_builder_column_1_1 1_1 fusion-flex-column fusion-flex-align-self-center\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-menu-anchor\" id=\"team3dsem\"><\/div><div class=\"fusion-title title fusion-title-8 fusion-sep-none fusion-title-text fusion-title-size-two\" style=\"--awb-font-size:45px;\"><h2 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;font-size:1em;\"><b>Team<br \/>\n<\/b><\/h2><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:20px;width:100%;\"><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-flex-end fusion-flex-justify-content-space-evenly fusion-flex-content-wrap\" style=\"width:104% !important;max-width:104% !important;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-4 fusion_builder_column_inner_1_4 1_4 fusion-flex-column fusion-flex-align-self-stretch\" style=\"--awb-bg-size:cover;--awb-width-large:25%;--awb-margin-top-large:0px;--awb-spacing-right-large:7.68%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:7.68%;--awb-width-medium:25%;--awb-order-medium:0;--awb-spacing-right-medium:7.68%;--awb-spacing-left-medium:7.68%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-image-element \" style=\"text-align:center;--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-none imageframe-3 hover-type-none\"><img decoding=\"async\" width=\"300\" height=\"449\" title=\"john-brumell-headshot-300\" src=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/john-brumell-headshot-300.png\" alt class=\"img-responsive wp-image-253\" srcset=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/john-brumell-headshot-300-200x299.png 200w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/john-brumell-headshot-300.png 300w\" sizes=\"(max-width: 640px) 100vw, 300px\" \/><\/span><\/div><div class=\"fusion-text fusion-text-16\" style=\"--awb-content-alignment:center;\"><p><a href=\"http:\/\/www.sickkids.ca\/AboutSickKids\/Directory\/People\/B\/John-Brumell.html\"><u>Dr. John Brumell<\/u><\/a><a href=\"mailto:john.brumell@sickkids.ca\"><u><br \/>\njohn.brumell@sickkids.ca<\/u><\/a><\/p>\n<\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-5 fusion_builder_column_inner_1_4 1_4 fusion-flex-column fusion-flex-align-self-stretch\" style=\"--awb-bg-size:cover;--awb-width-large:25%;--awb-margin-top-large:0px;--awb-spacing-right-large:7.68%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:7.68%;--awb-width-medium:25%;--awb-order-medium:0;--awb-spacing-right-medium:7.68%;--awb-spacing-left-medium:7.68%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-image-element \" style=\"text-align:center;--awb-aspect-ratio: 100 \/ 143;--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-none imageframe-4 hover-type-none has-aspect-ratio\"><img decoding=\"async\" width=\"296\" height=\"451\" title=\"Mei-Zhen-profile\" src=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2023\/10\/Mei-Zhen-profile.jpg\" class=\"img-responsive wp-image-478 img-with-aspect-ratio\" data-parent-fit=\"cover\" data-parent-container=\".fusion-image-element\" alt srcset=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2023\/10\/Mei-Zhen-profile-200x305.jpg 200w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2023\/10\/Mei-Zhen-profile.jpg 296w\" sizes=\"(max-width: 640px) 100vw, 296px\" \/><\/span><\/div><div class=\"fusion-text fusion-text-17\" style=\"--awb-content-alignment:center;\"><p><a href=\"https:\/\/www.lunenfeld.ca\/?page=zhen-mei\"><u>Dr. Mei Zhen<\/u><\/a><br \/><a href=\"pi@zhenlab.com)\"><u>pi@zhenlab.com<\/u><\/a><\/p>\n<\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-6 fusion_builder_column_inner_1_4 1_4 fusion-flex-column fusion-flex-align-self-stretch\" style=\"--awb-bg-size:cover;--awb-width-large:25%;--awb-margin-top-large:0px;--awb-spacing-right-large:7.68%;--awb-margin-bottom-large:20px;--awb-spacing-left-large:7.68%;--awb-width-medium:25%;--awb-order-medium:0;--awb-spacing-right-medium:7.68%;--awb-spacing-left-medium:7.68%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-image-element \" style=\"text-align:center;--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-none imageframe-5 hover-type-none\"><img decoding=\"async\" width=\"300\" height=\"451\" title=\"ali-darbandi\" src=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/ali-darbandi-1.png\" alt class=\"img-responsive wp-image-252\" srcset=\"https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/ali-darbandi-1-200x301.png 200w, https:\/\/lab.research.sickkids.ca\/nbif\/wp-content\/uploads\/sites\/98\/2020\/11\/ali-darbandi-1.png 300w\" sizes=\"(max-width: 640px) 100vw, 300px\" \/><\/span><\/div><div class=\"fusion-text fusion-text-18\" style=\"--awb-content-alignment:center;\"><p>Dr. Ali Darbandi, Facility Manager<br \/>\n<a href=\"mailto:ali.darbandi@sickkids.ca\"><u>ali.darbandi@sickkids.ca<\/u><\/a><br \/>\n<span style=\"color: #000000\">416-813-7654 ext. 309970<\/span><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":300,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"100-width.php","meta":{"footnotes":""},"class_list":["post-10","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>3D SEM - 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