PUBLICATIONS

A general system for targeting MHC class II–antigen complex via a single adaptable loop.

Du H, Liu J, Jude KM, Yang X, Li Y, Bell B, Yang H, Kassardjian A, Blackson W, Mobedi A, Parekh U, Sperberg A, Julien JP, Mellins E, Garcia KC, Huang P.

Nature Biotechnology. 2024 December 13. doi: 10.1038/s41587-024-02466-y.

bioRxiv. 2024 Jan 30. doi: 10.1101/2024.01.26.577489.

Human ITGAV variants are associated with immune dysregulation, brain abnormalities, and colitis.

Ghasempour S, Warner N, Guan R, Rodari MM, Ivanochko D, Whittaker Hawkins R, Marwaha A, Nowak JK, Liang Y, Mulder DJ, Stallard L, Li M, Yu DD, Pluthero FG, Batura V, Zhao M, Siddiqui I, Upton JEM, Hulst JM, Kahr WHA, Mendoza-Londono R, Charbit-Henrion F, Hoefsloot LH, Khiat A, Moreira D, Trindade E, do Céu Espinheira M, Pinto Pais I, Weerts MJA, Douben H, Kotlarz D, Snapper SB, Klein C, Dowling JJ., Julien JP, Joosten M, Cerf-Bensussan N, Freeman SA, Parlato M, van Ham TJ, Muise AM.

Journal of Experimental Medicine. 2024 November 11; 221(12): e20240546. doi: 10.1084/jem.20240546.

A human-serum-free medium can induce more infectious P. falciparum gametocytes than a conventional human-serum-containing medium.

Miura K, Deng B, Suresh RV, Gebremicale YT, Zhou L, Pham TP, Roche K, Diouf A, Lovell JF, Julien JP, Long CA.

Scientific Reports. 2024 September 27; 14, 22052. doi: 10.1038/s41598-024-73843-5.

A comparative immunological assessment of multiple clinical-stage adjuvants for the R21 malaria vaccine in nonhuman primates.

Arunachalam PS, Ha NY, Dennison SM, Spreng RL, Seaton KE, Xiao P, Feng Y, Zarnitsyna VI, Kazmin D, Hu M, Santagata JM, Xie X, Rogers K, Shirreff LM, Chottin C, Spencer AJ, Dutta S, Prieto K, Julien JP, Tomai M, Fox CB, Villinger F, Hill AVS, Tomaras GD, Pulendran B.

Science Translational Medicine. 2024 July 31; 16(758), adn6605. doi: 10.1126/scitranslmed.adn6605.

Humanization of pan-HLA-DR mAb 44H10 Hinges on Critical Residues in the Antibody Framework.

Kassardjian A, Ivanochko D, Barber B, Jetha A, Julien JP.

Antibodies. 2024 July 16; 13(3), 57. doi: 10.3390/antib13030057.

Disproportionate Rates of COVID-19 Among Black Canadian Communities: Lessons from a Cross-Sectional Study in the First Year of the Pandemic.

Allen UA, Barton M, Upton J, Bailey A, Campigotto A, Abdulnoor M, Julien JP, Gubbay J, Kissoon N, Litosh A, La Neve MR, Wong P, Allen A, Bailey R, Byrne W, Jagoowani R, Phillips C, Merreles Pulcini M, Polack A, Prescod C, Siddiqi A, Summers A, Thompson K, Thompson S, James C, for the seroMARK Research Group.

Journal of Racial and Ethnic Health Disparities. 2024 Jan 22. ofad500.1652. doi: 10.1007/s40615-023-01903-z.

Molecular determinants of cross-reactivity and potency by VH3-33 antibodies against the Plasmodium falciparum circumsporozoite protein.

Thai E, Murugan R, Binter S, Burn Aschner C, Prieto K, Kassardjian A, Obraztsova AS, Kang RW, Flores-Garcia Y, Mathis-Torres S, Li K, Horn GQ, Huntwork RHC, Bolscher JM, de Bruijni MHC, Sauerwein R, Dennison SM, Tomaras GD, Zavala F, Kellam P, Wardemann H, Julien JP.

Cell Reports. 2023 Oct 28; 42(11), 113330. doi: 10.1016/j.celrep.2023.113330.

A multi-specific, multi-affinity antibody platform neutralizes sarbecoviruses and confers protection against SARS-CoV-2 in vivo.

Burn Aschner C, Muthuraman K, Kucharska I, Cui H, Prieto K, Nair MS, Wang M, Huang Y, Christie-Holmes N, Poon B, Lam J, Sultana A, Kozak R, Mubareka S, Rubinstein JL, Rujas E, Treanor B, Ho DD, Jetha A, Julien JP.

Sci Transl Med. 2023 May 24; 15(697): eadf45. doi: 10.1126/scitranslmed.adf4549.

bioRxiv. 2022 Oct 24. doi: 10.1101/2022.10.23.513379.

Modular adjuvant-free pan-HLA-DR-immunotargeting subunit vaccine against SARS-CoV-2 elicits broad sarbecovirus-neutralizing antibody responses.

Kassardjian A, Sun E, Sookhoo J, Muthuraman K, Frias Boligan K, Kucharska I, Rujas E, Jetha A, Branch DR, Babiuk S, Barber B, Julien JP.

Cell Rep. 2023 Apr 25; 42(4): 112391. doi: 10.1016/j.celrep.2023.112391.

Molecular and functional properties of human Plasmodium falciparum CSP C-terminus antibodies.

Oludada OE, Costa G, Burn Aschner C, Obraztsova AS, Prieto K, Canetta C, Hoffman SL, Kremsner PG, Mordmüller B, Murugan R, Julien JP, Levashina EA, Wardemann H.

EMBO Mol Med. 2023 Apr 21; e17454. doi: 10.15252/emmm.202317454

bioRxiv. 2023 Jan 19. doi: 10.1101/2023.01.19.524692.

Glycosylated nanoparticle-based PfCSP vaccine confers long-lasting antibody responses and sterile protection in mouse malaria model.

Ludwig J, Scally SW, Costa G, Hoffmann S, Murugan R, Lossin J, Prieto K, Obraztcova A, Lobeto N, Franke-Fayard B, Janse CJ, Lebas C, Collin N, Binter S, Kellam P, Levashina EA, Wardemann H, Julien JP.

npj Vaccines. 2023 Apr 7; 8: 52. doi: 10.1038/s41541-023-00653-7.

Therapeutic targeting of LIF overcomes macrophage mediated immunosuppression of the local tumor microenvironment.

Hallett RM, Bonfill-Teixidor E, Iurlaro R, Arias A, Neva-Alejo A, Raman S, Bayliss P, Egorova O, McGray AJR, Lau E, Bosch A, Beilschmidt M, Choudhry A, Maetzel D, Fransson J, Huber-Ruano I, Anido J, Julien JP, Giblin P, Seoane J.

Clin Cancer Res. 2023 Feb 16; 29(4): 791-804. doi: 10.1158/1078-0432.CCR-21-1888.

Identification of highly potent transmission-blocking human monoclonal antibodies to Plasmodium falciparum Pfs230 in naturally exposed individuals.

Ivanochko D, Fabra-García A, Teelen K, van de Vegte-Bolmer M, van Gemert GJ, Newton J, Semesi A, de Bruijni M, Bolscher J, Ramjith J, Szabat M, Vogt S, Kraft L, Duncan S, Lee SM, Kamya MR, Feeney ME, Jagannathan P, Greenhouse B, Sauerwein RW, King CR, MacGill RS, Bousema T, Jore MM, Julien JP.

Immunity. 2023 Feb 14; 56(2): 420-432.e7. doi:10.1016/j.immuni.2023.01.013.

SSRN (Sneak Peek). 2022 Sep 8.

Highly potent naturally acquired human monoclonal antibodies against Pfs48/45 block Plasmodium falciparum transmission to mosquitoes.

Fabra-Garcia A, Hailemariam S, de Jong RM, Janssen K, Teelen K, van de Vegte-Bolmer M, van Gemert GJ, Ivanochko D, Semesi A, McLeod B, Vos MW, de Bruijni MHC, Bolscher JM, Szabat M, Vogt S, Kraft L, Duncan S, Kamya MR, Feeney ME, Jagannathan P, Greenhouse B, Sauerwein RW, King CR, MacGill RS, BousemaT, Julien JP, Jore MM.

Immunity. 2023 Feb 14; 56(2): 406-419.e7. doi:10.1016/j.immuni.2023.01.009.

SSRN (Sneak Peek). 2022 Sep 7.

High-density binding to Plasmodium falciparum circumsporozoite protein repeats by inhibitory antibody elicited in mouse with human immunoglobulin repertoire.

Kucharska I, Binter S, Murugan R, Scally SW, Ludwig J, Prieto K, Thai E, Costa G, Li K, Horn GQ, Flores-Garcia Y, Bosch A, Sicard T,  Rubinstein JL, Zavala F, Dennison SM, Tomaras GD, Levashina EA, Kellam P, Wardemann H, Julien JP.

PLOS Pathog. 2022 Nov 28. doi: 10.1371/journal.ppat.1010999.

Vaccination with a structure-based stabilized version of malarial antigen Pfs48/45 elicits ultra-potent transmission-blocking antibody responses.

McLeod B, Mabrouk MT, Miura K, Ravichandran R, Kephart S, Hailemariam S, Pham TP, Semesi A, Kucharska I, Kundu P, Huang WC, Johnson M, Blackstone A, Pettie D, Murphy M, Kraft JC, Leaf EM, Jiao Y, van de Vegte-Bolmer M, van Gemert GJ, Ramjith J, King CR, MacGill RS, Wu Y, Lee KK, Jore MM, King NP, Lovell JF, Julien JP.

Immunity. 2022 Sep 13; 55, 1-13. doi:10.1016/j.immuni.2022.07.015.

Dual inhibition of vacuolar ATPase and TMPRSS2 is required for complete blockade of SARS-CoV-2 entry into cells.

Icho S, Rujas E, Muthuraman K, Tam J, Liang H, Harms S, Liao M, Falzarano D, Julien JP, Melnyk R.

Antimicrob Agents Chemother. 2022 Jul 19; 66(7):e0043922. doi: 10.1128/aac.00439-22.

bioRxiv. 2022 Mar 14. doi: 10.1101/2022.03.11.484006.

Engineering pan–HIV-1 neutralization potency through multispecific antibody avidity.

Rujas E, Cui H, Burnie J, Aschner CB, Zhao T, Insausti S, Muthuraman K, Semesi A, Ophel J, Nieva JL, Seaman MS, Guzzo C, Treanor B, Julien JP.

Proc. Natl. Acad. Sci. U.S.A. 2022 Jan 21; 119(4):e2112887119. doi:10.1073/pnas.2112887119.

Structural basis of Plasmodium vivax inhibition by antibodies binding to the circumsporozoite protein repeats.

Kucharska I, Hossain L, Ivanochko D, Yang Q, Rubinstein JL, Pomès R, Julien JP.

eLife. 2022 Jan 13; 11:e72908. doi:10.7554/eLife.72908.

bioRxiv. 2021 Sep 1. doi: 10.1101/2021.08.31.458469.

GPC2-CAR T cells tuned for low antigen density mediate potent activity against neuroblastoma without toxicity.

Heitzeneder S, Bosse KR, Zhu Z, Zhelev D, Majzner RG, Radosevich MT, Dhingra S, Sotillo E, Buongervino S, Pascual-Pasto G, Garrigan E, Xu P, Huang J, Salzer B, Delaidelli A, Raman S, Cui H, Martinez B, Bornheimer SJ, Sahaf B, Alag A, Fetahu IS, Hasselblatt M, Parker KR, Anbunathan H, Hwang J, Huang M, Sakamoto K, Lacayo NJ, Klysz DD, Theruvath J, Vilches-Moure JG, Satpathy AT, Chang HY, Lehner M, Taschner-Mandl S, Julien JP, Sorensen PH, Dimitrov DS, Maris JM, Mackall CL.

Cancer Cell. 2022 Jan 10; 40(1):53-69.e9. doi: 10.1016/j.ccell.2021.12.005.

Multiscale interactome analysis coupled with off-target drug predictions reveals drug repurposing candidates for human coronavirus disease.

Sugiyama MG, Cui H, Redka DS, Karimzadeh M, Rujas E, Maan H, Hayat S, Cheung K, Misra R, McPhee JB, Viirre RD, Haller A, Botelho RJ, Karshafian R, Sabatinos SA, Fairn GD, Madani Tonekaboni SA, Windemuth A, Julien JP, Shahani V, MacKinnon SS, Wang B, Antonescu CN.

Sci Rep. 2021 Dec 2; 11, 1, 23315. doi: 10.1038/s41598-021-02432-7.

bioRxiv. 2021 Apr 13. doi: 10.1101/2021.04.13.439274.

Lyophilized, thermostable Spike or RBD immunogenic liposomes induce protective immunity against SARS-CoV-2 in mice.

Mabrouk MT, Chiem K, Rujas E, Huang WC, Jahagirdar D, Quinn B, Surendran Nair M, Nissly RH, Cavener VS, Boyle NR, Sornberger TA, Kuchipudi SV, Ortega J, Julien JP, Martinez-Sobrido L, Lovell J.

Sci Adv. 2021 Dec 1; 7, 49. doi: 10.1126/sciadv.abj1476.

Systematic engineering of optimized autonomous heavy-chain variable domains.

Nilvebrant J, Ereño-Orbea J, Gorelik M, Julian MC, Tessier PM, Julien JP, Sidhu SS.

J Mol Biol. 2021 Sep 9; 167241. doi: 10.1016/j.jmb.2021.167241.

Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile.

Rujas E, Leaman DP, Insausti S, Carravilla P, García-Porras M, Largo E, Morillo I, Sánchez-Eugenia R, Zhang L, Cui H, Iloro I, Elortza F, Julien JP, Eggeling C, Zwick MB, Caaveiro JMM, Nieva JL.

iScience. 2021 Aug 17; 102987. doi: 10.1016/j.isci.2021.102987.

A GPC2 antibody-drug conjugate is efficacious against neuroblastoma and small-cell lung cancer via binding a conformational epitope.

Raman S, Buongervino SN, Lane M, Zhelev DV, Zhu Z, Cui H, Martinez B, Martinez D, Wang Y, Upton K, Patel K, Rathi KS, Navia CT, Harmon DB, Pawel B, Dimitrov DS, Maris JM, Julien JP, Bosse KR.

Cell Rep Med. 2021 Jul 20; 2, 7, 100344. doi: 10.1016/j.xcrm.2021.100344.

Sneak Peak. 2020 Sep 18.

Structural details of monoclonal antibody m971 recognition of the membrane-proximal domain of CD22.

Ereño-Orbea J, Liu X, Sicard T, Kucharska I, Li W, Borovsky D, Cui H, Feng Y, Dimitrov DS, Julien JP.

J Biol Chem. 2021 Jul 14; 100966. doi: 10.1016/j.jbc.2021.100966.

Multivalency transforms SARS-CoV-2 antibodies into ultrapotent neutralizers.

Rujas E, Kucharska I, Tan YZ, Benlekbir S, Cui H, Zhao T, Wasney GA, Budylowski P, Guvenc F, Newton JC, Sicard T, Semesi A, Muthuraman K, Nouanesengsy A, Aschner CB, Prieto K, Bueler SA, Youssef S, Liao-Chan S, Glanville J, Christie-Holmes N, Mubareka S, Gray-Owen SD, Rubinstein JL, Treanor B, Julien JP.

Nat Commun. 2021 Jun 16; 12:3661. doi: 10.1038/s41467-021-23825.

bioRxiv. 2020 Oct 16. doi: 10.1101/2020.10.15.341636.

Video highlight of manuscript:

Polymeric assembly of endogenous Tuberous Sclerosis Protein Complex.

Dai DL, Hasan SNM, Woollard G, Bueler SA, Julien JP, Rubinstein JL, Mazhab-Jafari MT.

Biochemistry. 2021 Jun 3; 60, 23, 1808–1821. doi: 10.1021/acs.biochem.1c00269.

bioRxiv. 2021 Jan 19. doi: 10.1101/2021.01.18.426261.

Structural ordering of the Plasmodium berghei circumsporozoite protein repeats by inhibitory antibody 3D11.

Kucharska I, Thai E, Srivastava A, Rubinstein JL, Pomès R, Julien JP.

eLife. 2020 Nov 30; 9:e59018. doi:10.7554/eLife.59018.

bioRxiv. 2020 Jun 4. doi:10.1101/2020.06.02.131110.

B cell targeting by molecular adjuvants for enhanced immunogenicity.

Sicard T, Kassardjian A, Julien JP.

Expert Rev Vaccines. 2020 Nov 30. doi: 10.1080/14760584.2020.1857736.

Structural characterization of the ICOS/ICOS-L immune complex reveals high molecular mimicry by therapeutic antibodies.

Rujas E, Cui H, Sicard T, Semesi A, Julien JP.

Nat Commun. 2020 Oct 8; 11:5066. doi: 10.1038/s41467-020-18828-4.

A Versatile Soluble Siglec Scaffold for Sensitive and Quantitative Detection of Glycan Ligands.

Rodrigues E, Jung J, Park H, Loo C, Soukhtehzari S, Kitova EN, Mozaneh F, Daskhan G, Aghanya V, Sarkar S, Streith L, St. Laurent C, Julien JP, West LJ, Williams KC, Klassen JS, Macauley MS.

Nat Commun. 2020 Oct 8; 11:5091. doi: 10.1038/s41467-020-18907-6.

bioRxiv. 2020 Jul 29. doi: 10.1101/2020.07.28.223164.

Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments.

Rujas E, Insausti S, Leaman DP, Carravilla P, González-Resines S, Monceaux V, Sánchez-Eugenia R, García-Porras M, Iloro I, Zhang L, Elortza F, Julien JP, Saéz-Cirión A, Zwick MB, Eggeling C, Ojida A, Domene C, Caaveiro JMM, Nieva JL.

Cell Rep. 2020 Aug 18; 32 (7): 108037. doi: 10.1016/j.celrep.2020.108037.

A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity.

Thai E , Costa G, Weyrich A, Murugan R, Oyen D, Flores-Garcia Y, Prieto K, Bosch A, Valleriani A, Wu NC, Pholcharee T, Scally SW, Wilson IA, Wardemann H, Julien JP, Levashina EA.

J Exp Med. 2020 Aug 13; 217 (11): e20200061. doi: 10.1084/jem.20200061.

bioRxiv. 2020 Jan 14. doi: 10.1101/2020.01.13.904425.

Recognition of Semaphorin Proteins by P. sordellii Lethal Toxin Reveals Principles of Receptor Specificity in Clostridial Toxins.

Lee H, Beilhartz GL, Kucharska I, Raman S, Cui H, Lam M, Rubinstein JL, Schramek D, Julien JP, Melnyk RA, Taipale M.

Cell. 2020 Jul 23; 182 (2): 345–356.e16. doi: 10.1016/j.cell.2020.06.005.

bioRxiv. 2019 Dec 10. doi: 10.1101/871707.

Evolution of protective human antibodies against Plasmodium falciparum circumsporozoite protein repeat motifs.

Murugan R, Scally SW, Costa G, Mustafa G, Thai E, Decker T, Bosch A, Prieto K, Levashina EA, Julien JP, Wardemann H.

Nat Med. 2020 May 25; 26 (7):1135-1145. doi: 10.1038/s41591-020-0881-9.

bioRxiv. 2019 Oct 9. doi: 10.1101/798769.

De novo protein design enables the precise induction of RSV-neutralizing antibodies.

Sesterhenn F, Yang C, Bonet J, Cramer JT, Wen X, Wang Y, Chiang CI, Abriata LA, Kucharska I, Castoro G, Vollers SS, Galloux M, Dheilly E, Rosset S, Corthésy P, Georgeon S, Villard M, Richard CA, Descamps D, Delgado T, Oricchio E, Rameix-Welti MA, Más V, Ervin S, Eléouët JF, Riffault S, Bates JT, Julien JP, Li Y, Jardetzky T, Krey T, Correia BE.

Science. 2020 May 15; 368 (6492): eaay5051. doi: 10.1126/science.aay5051.

Potent antibody lineage against malaria transmission elicited by human vaccination with Pfs25.

McLeod B, Miura K, Scally SW, Bosch A, Nguyen N, Shin H, Kim D, Volkmuth W, Rämisch S, Chichester JA, Streatfield S, Woods C, Schief WR, Emerling D, King CR, Julien JP.

Nat Commun. 2019 Sep 24; 10: 4328. doi: 10.1038/s41467-019-11980-6.

Antibodies against Plasmodium falciparum malaria at the molecular level.

Julien JP, Wardemann H.

Nat Rev Immunol. 2019 Aug 28; 19: 761-775. doi: 10.1038/ s41577-019-0209-5.

Cholesterol Interaction Directly Enhances Intrinsic Activity of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).

Chin S, Ramjeesingh M, Hung M, Ereño-Oreba J, Cui H, Laselva O, Julien JP, Bear CE.

Cells. 2019 Jul 31;8(8). pii: E804. doi: 10.3390/cells8080804.

bioRxiv. 2018 Dec 16. doi: 10.1101/495010.

N-linked glycosylation regulates CD22 organization and function.

Wasim L, Buhari FH, Yoganathan M, Sicard T, Ereno-Orbea J, Julien JP, Treanor B.

Front. Immunol. 2019 Mar 14; 10: 699. doi: 10.3389/fimmu.2019.00699.

Structure-guided design fine-tunes pharmacokinetics, tolerability, and antitumor profile of multispecific frizzled antibodies.

Raman S, Beilschmidt M, To M, Lin K, Lui F, Jmeian Y, Ng M, Fernandez M, Fu Y, Mascall K, Duque A, Wang X, Pan G, Angers S, Moffat J, Sidhu SS, Magram J, Sinclair AM, Fransson J, Julien JP.

Proc. Natl. Acad. Sci. U.S.A. 2019 Mar 20; 116(14): 6812-6817. doi: 10.1073/pnas.1817246116.

Molecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions.

Carravilla P, Chojnacki J, Rujas E, Insausti S, Largo E, Waithe D, Apellaniz B, Sicard T, Julien JP, Eggeling C, Nieva JL.

Nat Commun. 2019 Jan 8; 10: 78. doi: 10.1038/s41467-018-07962-9.