{"id":104,"date":"2015-11-09T20:17:41","date_gmt":"2015-11-09T20:17:41","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/howell\/?page_id=104"},"modified":"2018-04-19T09:43:46","modified_gmt":"2018-04-19T14:43:46","slug":"psl","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/howell\/molecular-mechanisms\/exopolysaccharides\/psl\/","title":{"rendered":"Psl"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-overflow:visible;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_4 1_4 fusion-one-fourth fusion-column-first\" style=\"--awb-bg-size:cover;width:22%; margin-right: 4%;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-image-element in-legacy-container\" style=\"--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-none imageframe-1 hover-type-none\"><a href=\"https:\/\/lab.research.sickkids.ca\/howell\/wp-content\/uploads\/sites\/17\/2015\/11\/Psl_model.jpeg\" class=\"fusion-lightbox\" data-rel=\"iLightbox[3bf09136b3d2f3d74e4]\" data-title=\"Psl_model\" title=\"Psl_model\"><img decoding=\"async\" width=\"938\" height=\"1311\" alt=\"Model of Psl biosynthesis\" src=\"https:\/\/lab.research.sickkids.ca\/howell\/wp-content\/uploads\/sites\/17\/2015\/11\/Psl_model.jpeg\" class=\"img-responsive wp-image-912\" srcset=\"https:\/\/lab.research.sickkids.ca\/howell\/wp-content\/uploads\/sites\/17\/2015\/11\/Psl_model-215x300.jpeg 215w, https:\/\/lab.research.sickkids.ca\/howell\/wp-content\/uploads\/sites\/17\/2015\/11\/Psl_model-733x1024.jpeg 733w, https:\/\/lab.research.sickkids.ca\/howell\/wp-content\/uploads\/sites\/17\/2015\/11\/Psl_model-768x1073.jpeg 768w, https:\/\/lab.research.sickkids.ca\/howell\/wp-content\/uploads\/sites\/17\/2015\/11\/Psl_model.jpeg 938w\" sizes=\"(max-width: 938px) 100vw, 938px\" \/><\/a><\/span><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-bottom:10px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-text fusion-text-1\"><p>Model of Psl\u00a0biosynthetic machinery<\/p>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_3_4 3_4 fusion-three-fourth fusion-column-last\" style=\"--awb-bg-size:cover;width:74%;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-2\"><p>Psl is the predominant polysaccharide found in biofilm infections in clinical and environmental settings. Psl acts as an adhesin and plays an important role in the formation and maintenance of the biofilm architecture in <em>P. aeruginosa <\/em>infections. The polymer provides protection against the immune system and is a first line of defense during the initial stages of biofilm development. PSL is composed of a pentasaccharide-repeating unit of D-mannose, D-glucose and L-rhamnose. While the mechanistic details of how the polymer is produced are not fully defined, it is proposed to occur via a Wzx\/Wzy-dependent mechanism, similar to the <em>E.<\/em><em> coli<\/em> group 1 capsular polysaccharides.<\/p>\n<p>We have recently shown that deletion of <em>pslG <\/em>does not impact Psl synthesis or biofilm formation and have demonstrated that PslG is an active <a href=\"https:\/\/lab.research.sickkids.ca\/howell\/therapeutics\/glycoside-hydrolases\/\">glycoside hydrolase<\/a>.<\/p>\n<\/div><div class=\"fusion-text fusion-text-3\"><h2>Current projects<\/h2>\n<p>Ongoing projects are focused on:<\/p>\n<ul>\n<li>Determining the role of the PslG in polymer biosynthesis.<\/li>\n<li><a href=\"https:\/\/lab.research.sickkids.ca\/howell\/therapeutics\/glycoside-hydrolases\/\">Developing PslG as a therapeutic<\/a> for the prevention and degradation of PSL-dependent biofilms.<\/li>\n<\/ul>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-2 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last fusion-column-no-min-height\" style=\"--awb-bg-size:cover;--awb-margin-bottom:0px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-3 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last fusion-column-no-min-height\" style=\"--awb-bg-size:cover;--awb-margin-bottom:0px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-text fusion-title-size-two\"><h2 class=\"fusion-title-heading title-heading-left\" style=\"margin:0;\">Selected Publications<\/h2><\/div><div class=\"fusion-text fusion-text-4\"><div class=\"rprt\">\n<ul>\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26424791\" target=\"_blank\" rel=\"noopener\">Characterization of the Pseudomonas aeruginosa Glycoside Hydrolase PslG Reveals That Its Levels Are Critical for Psl Polysaccharide Biosynthesis and Biofilm Formation.<\/a>\u00a0Baker P, Whitfield GB, Hill PJ, Little DJ, Pestrak MJ, Robinson H, Wozniak DJ, Howell PL.\u00a0<span class=\"jrnl\" title=\"The Journal of biological chemistry\">J Biol Chem<\/span>. 2015 Nov 20;290(47):28374-87. doi: 10.1074\/jbc.M115.674929.<\/li>\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21991261\" target=\"_blank\" rel=\"noopener\">Biosynthesis of the Pseudomonas aeruginosa Extracellular Polysaccharides, Alginate, Pel, and Psl.<\/a>\u00a0Franklin MJ, Nivens DE, Weadge JT, Howell PL.\u00a0<span class=\"jrnl\" title=\"Frontiers in microbiology\">Front Microbiol<\/span>. 2011 Aug 22;2:167. doi: 10.3389\/fmicb.2011.00167.<\/li>\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22176658\" target=\"_blank\" rel=\"noopener\">The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix.<\/a>\u00a0Colvin KM, Irie Y, Tart CS, Urbano R, Whitney JC, Ryder C, Howell PL, Wozniak DJ, Parsek MR.\u00a0<span class=\"jrnl\" title=\"Environmental microbiology\">Environ Microbiol<\/span>. 2012 Aug;14(8):1913-28. doi: 10.1111\/j.1462-2920.2011.02657.x.<\/li>\n<\/ul>\n<\/div>\n<\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":95,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-104","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - 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