{"id":645,"date":"2017-10-30T19:59:37","date_gmt":"2017-10-30T19:59:37","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/fcf\/?page_id=645"},"modified":"2020-04-23T23:04:22","modified_gmt":"2020-04-23T23:04:22","slug":"automacs-pro","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/fcf\/automacs-pro\/","title":{"rendered":"AutoMACS Pro"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_3_4 3_4 fusion-three-fourth fusion-column-first\" style=\"--awb-bg-size:cover;width:75%;width:calc(75% - ( ( 4% ) * 0.75 ) );margin-right: 4%;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-1\"><h3>autoMACS Pro Cell Separator<\/h3>\n<p><strong>Location: PMCRT 2-504<\/strong><\/p>\n<p>The autoMACS Pro Separator is a bench-top automated magnetic cell separator used for cell isolation and enrichment. Up to six samples can be processed automatically in volumes ranging from 0.2 mL to 50 mL. Preset cell separation programs allow positive or negative selection of magnetically labeled cells using MACS columns.<\/p>\n<p>Cell separation programs are chosen based on levels of antigen expression and desired levels of recovery and purity. Cells are first labeled by the user with antibodies and\/or MACS magnetic microbeads and then loaded onto the autoMACS where cells are passed through one or two columns (depending on the program). Both labeled and non-labeled fractions are eluted into tubes in chilled tube racks. Wash programs are performed between separations. The unit is in a bio-safety cabinet for aseptic handling of samples.<\/p>\n<p>For more info visit: <a href=\"http:\/\/www.automacs.info\/\" target=\"_blank\" rel=\"noopener noreferrer\">autoMACS<\/a> and <a href=\"http:\/\/www.miltenyibiotec.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Miltenyi Biotec<\/a>.<\/p>\n<p><strong>Key features<\/strong><\/p>\n<ul>\n<li>10 standard programs<\/li>\n<li>Program duration: 2.5-10 minutes per sample mL, 4 minutes per wash<\/li>\n<li>Maximum number of cells: 2 x 10<sup>8<\/sup> labeled cells, 4 x 10<sup>9<\/sup> total cells per separation<\/li>\n<li>Minimum number of cells: 1 x 10<sup>5<\/sup><\/li>\n<li>Sample volume: 200 \u00b5l to 50 mL<\/li>\n<\/ul>\n<p><strong>User provides<\/strong><\/p>\n<ul>\n<li>Running buffer: can be purchased from Miltenyi Biotec (PBS, 2mM EDTA, 0.5%BSA, 0.09% NaAzide, cat#130-091-221, $330 for 6 x 1.5L) or make your own in 1L pyrex bottles<\/li>\n<li>MACS microbeads and antibodies for labeling<\/li>\n<\/ul>\n<\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:10px;margin-bottom:10px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_4 1_4 fusion-one-fourth fusion-column-last\" style=\"--awb-bg-size:cover;width:25%;width:calc(25% - ( ( 4% ) * 0.25 ) );\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:25px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-button-wrapper\"><a class=\"fusion-button button-flat fusion-button-default-size button-default fusion-button-default button-1 fusion-button-default-span fusion-button-default-type\" target=\"_self\" href=\"https:\/\/lab.research.sickkids.ca\/fcf\/instruments\/\"><span class=\"fusion-button-text awb-button__text awb-button__text--default\">Back to Instruments<\/span><\/a><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:25px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-image-element in-legacy-container\" style=\"--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><span class=\" fusion-imageframe imageframe-glow imageframe-1 hover-type-none\" style=\"-webkit-box-shadow: 0 0 3px rgba(0,0,0,0.3);box-shadow: 0 0 3px rgba(0,0,0,0.3);\"><img decoding=\"async\" width=\"300\" height=\"200\" alt=\"automacs magnetic cell purification\" title=\"automacs\" src=\"https:\/\/lab.research.sickkids.ca\/fcf\/wp-content\/uploads\/sites\/83\/2017\/10\/automacs.jpg\" class=\"img-responsive wp-image-514\" srcset=\"https:\/\/lab.research.sickkids.ca\/fcf\/wp-content\/uploads\/sites\/83\/2017\/10\/automacs-200x133.jpg 200w, https:\/\/lab.research.sickkids.ca\/fcf\/wp-content\/uploads\/sites\/83\/2017\/10\/automacs.jpg 300w\" sizes=\"(max-width: 800px) 100vw, 300px\" \/><\/span><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-645","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - 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