{"id":396,"date":"2017-10-25T18:40:50","date_gmt":"2017-10-25T18:40:50","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/fcf\/?page_id=396"},"modified":"2023-01-09T20:59:25","modified_gmt":"2023-01-09T20:59:25","slug":"flow-cytometry","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/fcf\/flow-cytometry\/","title":{"rendered":"Instruments"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:20px;--awb-padding-bottom:20px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last\" style=\"--awb-bg-size:cover;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-1\"><h3>Flow Cytometry Instruments<\/h3>\n<\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:10px;margin-bottom:10px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-tabs fusion-tabs-1 classic nav-not-justified horizontal-tabs icon-position-left mobile-mode-accordion\" style=\"--awb-title-border-radius-top-left:0px;--awb-title-border-radius-top-right:0px;--awb-title-border-radius-bottom-right:0px;--awb-title-border-radius-bottom-left:0px;--awb-alignment:start;--awb-inactive-color:#ebeaea;--awb-background-color:#ffffff;--awb-border-color:#ebeaea;--awb-active-border-color:#64a0c8;\"><div class=\"nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li class=\"active\" role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-c10a359905bf5a9e61c\" aria-selected=\"true\" id=\"fusion-tab-cellsorters\" href=\"#tab-c10a359905bf5a9e61c\"><h4 class=\"fusion-tab-heading\">Cell Sorters<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-d74fd5c049e4cc3f394\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-analyzers\" href=\"#tab-d74fd5c049e4cc3f394\"><h4 class=\"fusion-tab-heading\">Analyzers<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-9bb72a5fc89932b698a\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-magneticcellseparators\" href=\"#tab-9bb72a5fc89932b698a\"><h4 class=\"fusion-tab-heading\">Magnetic Cell Separators<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-content\"><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li class=\"active\" role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-c10a359905bf5a9e61c\" aria-selected=\"true\" id=\"mobile-fusion-tab-cellsorters\" href=\"#tab-c10a359905bf5a9e61c\"><h4 class=\"fusion-tab-heading\">Cell Sorters<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix in active\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-cellsorters\" id=\"tab-c10a359905bf5a9e61c\">\n<p>Our cell sorters have different optical configurations (laser, detectors &amp; filters) and use different sorting technologies in order to accommodate many panels and applications. Click on the instruments below for detailed optical configuration information.<\/p>\n<div class=\"contentbox1\">\n<p>Clients must provide sample biosafety information for every cell sorting reservation.<\/p>\n<ul>\n<li>The FCF operates in <strong>Containment Level 2 (CL2) laboratories<\/strong> and can handle samples designated as RG1 or RG2.<\/li>\n<li>All of our cell sorters are installed in biosafety cabinets.<\/li>\n<li><strong>Samples or agents designated as RG3 and RG4 are prohibited from FCF labs. <\/strong><\/li>\n<li>More information about FCF Biosafety Policies can be found in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\" target=\"_blank\" rel=\"noopener noreferrer\">iLab<\/a>.<\/li>\n<\/ul>\n<\/div>\n<h5>BD Biosciences Cell Sorters<\/h5>\n\n<table id=\"tablepress-6\" class=\"tablepress tablepress-id-6 tablepress-responsive tbody-has-connected-cells\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Instrument<\/th><th class=\"column-2\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-facsaria-iii-cfi-byrv\/\">Aria III CFI<\/a><\/th><th class=\"column-3\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-facsaria-fusion-a-bgrv\/\">Aria Fusion A<\/a><\/th><th class=\"column-4\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-facsaria-fusion-b-bgrv\/\">Aria Fusion B<\/a><\/th><th class=\"column-5\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-symphony-s6-a-uvbyr\">Symphony S6 A<\/a><\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><strong>Location<\/strong><\/td><td class=\"column-2\">PMCRT, 2-203<\/td><td class=\"column-3\">PMCRT, 2-504<\/td><td class=\"column-4\">PMCRT, 2-203<\/td><td class=\"column-5\">PMCRT, 2-203<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><strong># of colours<\/strong><\/td><td class=\"column-2\">18<\/td><td class=\"column-3\">16<\/td><td class=\"column-4\">16<\/td><td class=\"column-5\">30<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td colspan=\"5\" class=\"column-1\"><strong>LASERS<\/strong><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><strong>UV<\/strong><\/td><td class=\"column-2\">-<\/td><td class=\"column-3\">-<\/td><td class=\"column-4\">-<\/td><td class=\"column-5\">60 mW<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><strong>Violet<\/strong><\/td><td class=\"column-2\">50 mW<\/td><td class=\"column-3\">85 mW<\/td><td class=\"column-4\">85 mW<\/td><td class=\"column-5\">200 mW<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><strong>Blue<\/strong><\/td><td class=\"column-2\">20 mW<\/td><td class=\"column-3\">50 mW<\/td><td class=\"column-4\">50 mW<\/td><td class=\"column-5\">200 mW<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><strong>(Yellow) Green<\/strong><\/td><td class=\"column-2\">50 mW (561 nm)<\/td><td class=\"column-3\">150 mW (532 nm)<\/td><td class=\"column-4\">150 mW (532 nm)<\/td><td class=\"column-5\">150 mW (561 nm)<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><strong>Red<\/strong><\/td><td class=\"column-2\">15 mW<\/td><td class=\"column-3\">100 mW<\/td><td class=\"column-4\">100 mW<\/td><td class=\"column-5\">140 mW<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-6 from cache -->\n<h5>Sony Cell Sorters<\/h5>\n\n<table id=\"tablepress-55\" class=\"tablepress tablepress-id-55 tablepress-responsive tbody-has-connected-cells\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Instrument<\/th><th class=\"column-2\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/sony-ma900-bryv\/\">Sony MA900 <\/a><\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><strong>Location<\/strong><\/td><td class=\"column-2\">PGCRL, 18.9430<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><strong># of colours<\/strong><\/td><td class=\"column-2\">12<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td colspan=\"2\" class=\"column-1\"><strong>LASERS (co-linear)<\/strong><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><strong>Violet \/ Blue \/ Red<\/strong><\/td><td class=\"column-2\">-<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><strong>Blue \/ Yellow-Green<\/strong><\/td><td class=\"column-2\">36 mW \/ 36 mW (561 nm)<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><strong>Violet \/ Red<\/strong><\/td><td class=\"column-2\">10 mW \/ 36 mW<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-55 from cache -->\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-d74fd5c049e4cc3f394\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-analyzers\" href=\"#tab-d74fd5c049e4cc3f394\"><h4 class=\"fusion-tab-heading\">Analyzers<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-analyzers\" id=\"tab-d74fd5c049e4cc3f394\">\n<p>Our analyzers have different optical configurations (laser, detectors &amp; filters) designed to accommodate many panels and applications. Click on the instruments below for detailed optical configuration information.<\/p>\n<p>The LSR Fortessa and Symphony A3 have a <strong>High Throughput Sampler (HTS)<\/strong> option. The HTS unit allows for acquisition from 96- or 384-well plates. Additional <a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/instrument-training\/\">training<\/a> is required to use the HTS option.<\/p>\n<ul>\n<li>To request training, submit an <strong>Analyzer HTS Training<\/strong> Request (listed under the Training Requests category) in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\" target=\"_blank\" rel=\"noopener noreferrer\">iLab<\/a>.<\/li>\n<\/ul>\n<p>Clients must provide sample biosafety information for every analytical reservation.<\/p>\n<ul>\n<li>The FCF operates in <strong>Containment Level 2 (CL2) laboratories<\/strong> and can handle samples designated as RG1 or RG2.<\/li>\n<li><strong>Samples or agents designated as RG3 and RG4 are prohibited from FCF labs. <\/strong><\/li>\n<li>More information about FCF Biosafety Policies can be found in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\" target=\"_blank\" rel=\"noopener noreferrer\">iLab<\/a>.<\/li>\n<\/ul>\n\n<table id=\"tablepress-5\" class=\"tablepress tablepress-id-5 tablepress-responsive tbody-has-connected-cells\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Instrument<\/th><th class=\"column-2\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-lsr-fortessa\">Fortessa<\/a><\/th><th class=\"column-3\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-fortessab\">Fortessa B<\/a><\/th><th class=\"column-4\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-symphonya3_pmcrt\/\">Symphony A3 A<\/a><\/th><th class=\"column-5\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/bd-symphonya3_pmcrt\/\">Symphony A3 C<\/a><\/th><th class=\"column-6\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/symphony-a3-b\/\">Symphony A3 B<\/a><\/th><th class=\"column-7\"><a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/lsr-ii-gc-brvu\/\">LSRII GC<\/a><\/th><th class=\"column-8\"><a href=\"https:\/\/app.fluorofinder.com\/experiments\/47f241100b00013d3b400211f09e3eab\/machine\" rel=\"noopener\" target=\"_blank\">Symphony A5SE<\/a><\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><strong>Location<\/strong><\/td><td class=\"column-2\">PGCRL, 18.9430<\/td><td class=\"column-3\">PMCRT, 2-203<\/td><td class=\"column-4\">PMCRT, 2-504<\/td><td class=\"column-5\">PMCRT, 2-504<\/td><td class=\"column-6\">PGCRL, 18.9430<\/td><td class=\"column-7\">PGCRL, 18.9430<\/td><td class=\"column-8\">PMCRT, 2-504<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><strong># of colours<\/strong><\/td><td class=\"column-2\">17<\/td><td class=\"column-3\">18<\/td><td class=\"column-4\">28<\/td><td class=\"column-5\">28<\/td><td class=\"column-6\">28<\/td><td class=\"column-7\">12<\/td><td class=\"column-8\">48<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><strong>HTS unit<\/strong><\/td><td class=\"column-2\">Yes<\/td><td class=\"column-3\">Yes<\/td><td class=\"column-4\">Yes<\/td><td class=\"column-5\">Yes<\/td><td class=\"column-6\">Yes<\/td><td class=\"column-7\">No<\/td><td class=\"column-8\">Yes<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td colspan=\"8\" class=\"column-1\"><strong>LASERS<\/strong><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><strong>UV<\/strong><\/td><td class=\"column-2\">20 mW<\/td><td class=\"column-3\">30 mW<\/td><td class=\"column-4\">45 mW<\/td><td class=\"column-5\">45 mW<\/td><td class=\"column-6\">60 mW<\/td><td class=\"column-7\">-<\/td><td class=\"column-8\">60 mW<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><strong>Violet<\/strong><\/td><td class=\"column-2\">50 mW<\/td><td class=\"column-3\">50 mW<\/td><td class=\"column-4\">100 mW<\/td><td class=\"column-5\">100 mW<\/td><td class=\"column-6\">100 mW<\/td><td class=\"column-7\">50 mW<\/td><td class=\"column-8\">200 mW<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><strong>Blue<\/strong><\/td><td class=\"column-2\">100 mW<\/td><td class=\"column-3\">50 mW<\/td><td class=\"column-4\">100 mW<\/td><td class=\"column-5\">100 mW<\/td><td class=\"column-6\">100 mW<\/td><td class=\"column-7\">100 mW<\/td><td class=\"column-8\">150 mW<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><strong>(Yellow) Green<\/strong><\/td><td class=\"column-2\">150 mW (532 nm)<\/td><td class=\"column-3\">150 mW (532 nm)<\/td><td class=\"column-4\">100 mW (561 nm)<\/td><td class=\"column-5\">100 mW (561 nm)<\/td><td class=\"column-6\">100 mW (561 nm)<\/td><td class=\"column-7\">-<\/td><td class=\"column-8\">150 mW (561 nm)<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><strong>Red<\/strong><\/td><td class=\"column-2\">40 mW <\/td><td class=\"column-3\">60 mW <\/td><td class=\"column-4\">140 mW <\/td><td class=\"column-5\">140 mW <\/td><td class=\"column-6\">140 mW <\/td><td class=\"column-7\">20 mW<\/td><td class=\"column-8\">140 mW <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-5 from cache -->\n<p>For access please submit request in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\" target=\"_blank\" rel=\"noopener noreferrer\">iLab<\/a>.<\/p>\n<div class=\"accordian fusion-accordian\" style=\"--awb-border-size:1px;--awb-icon-size:13px;--awb-content-font-size:14px;--awb-icon-alignment:left;--awb-hover-color:#f9f9f9;--awb-border-color:#cccccc;--awb-background-color:#ffffff;--awb-divider-color:#e0dede;--awb-divider-hover-color:#e0dede;--awb-icon-color:#ffffff;--awb-title-color:#64a0c8;--awb-content-color:#6b6b6b;--awb-icon-box-color:#333333;--awb-toggle-hover-accent-color:#64a0c8;--awb-title-font-family:Arial, Helvetica, sans-serif;--awb-title-font-weight:400;--awb-title-font-style:normal;--awb-title-font-size:18px;--awb-content-font-family:Arial, Helvetica, sans-serif;--awb-content-font-style:normal;--awb-content-font-weight:400;\"><div class=\"panel-group fusion-toggle-icon-boxed\" id=\"accordion-396-1\"><div class=\"fusion-panel panel-default panel-aedd5772157fc1981 fusion-toggle-has-divider\"><div class=\"panel-heading\"><h4 class=\"panel-title toggle\" id=\"toggle_aedd5772157fc1981\"><a aria-expanded=\"false\" aria-controls=\"aedd5772157fc1981\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-396-1\" data-target=\"#aedd5772157fc1981\" href=\"#aedd5772157fc1981\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Additional Filters<\/span><\/a><\/h4><\/div><div id=\"aedd5772157fc1981\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_aedd5772157fc1981\"><div class=\"panel-body toggle-content fusion-clearfix\">\n<p>Each analyzer has its own default configuration designed for a specific set of commonly used fluorochromes in which all detectors are used simultaneously. Filters in this configuration may not necessarily be optimal for your particular fluorochrome combination. Additional filters are available and can be signed out from the FCF office.<\/p>\n<ul>\n<li>To request alternative filters, submit a <strong>General Assistance<\/strong> Request (listed under the General Requests category) in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\">iLab<\/a>.<\/li>\n<\/ul>\n<p>The instruments must be returned to their default configuration after use.<\/p>\n<ul>\n<li>A member of our staff will assist you to sign out the filter(s) you require during normal working hours (Monday to Friday, 10 a.m. to 6 p.m.). Please return the filter(s) to a member of the our staff at the end of your appointment.<\/li>\n<li>If your appointment occurs after hours or on the weekends, the filter(s) must be returned by 10 a.m. the next working day.<\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><\/div>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-9bb72a5fc89932b698a\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-magneticcellseparators\" href=\"#tab-9bb72a5fc89932b698a\"><h4 class=\"fusion-tab-heading\">Magnetic Cell Separators<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-magneticcellseparators\" id=\"tab-9bb72a5fc89932b698a\">\n<h5>Magnetic Cell Separators<\/h5>\n<p>Magnetic cell separation technology can be used to enrich heterogeneous cell samples for particular cell populations prior to sorting, or as an endpoint itself (e.g. positive selection of CD11c+ DC, generation of Lineage negative cells for adoptive transfer).<\/p>\n<p>Our facility has an automated magnetic separator, autoMACS Pro Separator (Miltenyi Biotec), located in room 2-504 at PMCRT. A<a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/instrument-training\/\" target=\"_blank\" rel=\"noopener noreferrer\"> mandatory 15-minute training session provided by FCF staff is a prerequisite<\/a>.<\/p>\n<h5>autoMACS Pro Separator<\/h5>\n<p><strong>Location:<\/strong> PMCRT 2-504<\/p>\n<p><img decoding=\"async\" class=\"size-full wp-image-514 alignleft\" src=\"https:\/\/lab.research.sickkids.ca\/fcf\/wp-content\/uploads\/sites\/83\/2017\/10\/automacs.jpg\" alt=\"\" width=\"300\" height=\"200\" srcset=\"https:\/\/lab.research.sickkids.ca\/fcf\/wp-content\/uploads\/sites\/83\/2017\/10\/automacs-200x133.jpg 200w, https:\/\/lab.research.sickkids.ca\/fcf\/wp-content\/uploads\/sites\/83\/2017\/10\/automacs.jpg 300w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>The autoMACS Pro Separator is a bench-top automated magnetic cell separator used for cell isolation and enrichment. Up to six samples can be processed automatically in volumes ranging from 0.2 mL to 50 mL. Preset cell separation programs allow positive or negative selection of magnetically labeled cells using MACS columns.<\/p>\n<p>Cell separation programs are chosen based on levels of antigen expression and desired levels of recovery and purity. Cells are first labeled by the user with antibodies and\/or MACS magnetic microbeads and then loaded onto the autoMACS where cells are passed through one or two columns (depending on the program). Both labeled and non-labeled fractions are eluted into tubes in chilled tube racks. Wash programs are performed between separations. The unit is in a bio-safety cabinet for aseptic handling of samples.<\/p>\n<p>Clients must provide sample biosafety information for every autoMACS reservation.<\/p>\n<ul>\n<li>The FCF operates in <strong>Containment Level 2 (CL2) laboratories<\/strong> and can handle samples designated as RG1 or RG2.<\/li>\n<li><strong>Samples or agents designated as RG3 and RG4 are prohibited from FCF labs. <\/strong><\/li>\n<li>More information about FCF Biosafety Policies can be found in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\">iLab<\/a>.<\/li>\n<\/ul>\n<p><strong>Key features:<\/strong><\/p>\n<ul>\n<li>10 standard programs<\/li>\n<li>Program duration: 2.5-10 minutes per sample mL, 4 minutes per wash<\/li>\n<li>Maximum number of cells: 2 x 10<sup>8<\/sup> labeled cells, 4 x 10<sup>9<\/sup> total cells per separation<\/li>\n<li>Minimum number of cells: 1 x 10<sup>5<\/sup><\/li>\n<li>Sample volume: 200 \u00b5l to 50 mL<\/li>\n<\/ul>\n<p><strong>User provides:<\/strong><\/p>\n<ul>\n<li>Running buffer: can be purchased from Miltenyi (PBS, 2 mM EDTA, 0.5% BSA, 0.09% NaAzide, cat#130-091-221, $330 for 6 x 1.5L) or make your own in 1L pyrex bottles<\/li>\n<li>MACS microbeads and antibodies for labeling<\/li>\n<\/ul>\n<\/div><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-396","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - 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