{"id":142,"date":"2017-10-17T19:22:07","date_gmt":"2017-10-17T19:22:07","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/fcf\/?page_id=142"},"modified":"2024-06-26T17:38:22","modified_gmt":"2024-06-26T17:38:22","slug":"instrument-training","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/fcf\/instrument-training\/","title":{"rendered":"Instrument Training"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-padding-top:20px;--awb-padding-bottom:20px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last\" style=\"--awb-bg-size:cover;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-1\"><h1>Training Courses<\/h1>\n<p>At the SickKids-UHN FCF, we are committed to implementing best practices in the field and ensuring that our clients have the requisite technical knowledge and tools to generate high quality, reproducible data.<\/p>\n<p>We offer online and hands-on training courses for clients to broaden their flow cytometry knowledge and\/or operate our instruments independently.<\/p>\n<\/div><div class=\"accordian fusion-accordian\" style=\"--awb-border-size:1px;--awb-icon-size:13px;--awb-content-font-size:14px;--awb-icon-alignment:left;--awb-hover-color:#f9f9f9;--awb-border-color:#cccccc;--awb-background-color:#ffffff;--awb-divider-color:#e0dede;--awb-divider-hover-color:#e0dede;--awb-icon-color:#ffffff;--awb-title-color:#64a0c8;--awb-content-color:#6b6b6b;--awb-icon-box-color:#333333;--awb-toggle-hover-accent-color:#64a0c8;--awb-title-font-family:Arial, Helvetica, sans-serif;--awb-title-font-weight:400;--awb-title-font-style:normal;--awb-title-font-size:18px;--awb-title-line-height:1.5;--awb-content-font-family:Arial, Helvetica, sans-serif;--awb-content-font-style:normal;--awb-content-font-weight:400;\"><div class=\"panel-group fusion-toggle-icon-boxed\" id=\"accordion-142-1\"><div class=\"fusion-panel panel-default panel-c448a743d6ef69376 fusion-toggle-has-divider\" style=\"--awb-title-color:#64a0c8;--awb-content-color:#6b6b6b;\"><div class=\"panel-heading\"><h4 class=\"panel-title toggle\" id=\"toggle_c448a743d6ef69376\"><a aria-expanded=\"false\" aria-controls=\"c448a743d6ef69376\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-142-1\" data-target=\"#c448a743d6ef69376\" href=\"#c448a743d6ef69376\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">FluoroFinder Online Training Course<\/span><\/a><\/h4><\/div><div id=\"c448a743d6ef69376\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_c448a743d6ef69376\"><div class=\"panel-body toggle-content fusion-clearfix\">\n<p>FluoroFinder hosts an online, on-demand streamlined educational program. The courses are delivered as subtitled video-based micro-content and simulation and can be completed in 2-3 h (total).<\/p>\n<ul>\n<li><strong>Flow Cytometry Essentials (FCE) <\/strong>provides a comprehensive introduction to the basic principles and concepts of flow cytometry:\n<ul>\n<li>Section 1: Principles &amp; Instrumentation<\/li>\n<li>Section\u00a02:\u00a0Reagents &amp; Panel Design<\/li>\n<li>Section\u00a03: Acquisition &amp; Measurements<\/li>\n<li>Section\u00a04: Analysis (with Interactive Tools)<\/li>\n<li>Section 5: Statistics<\/li>\n<\/ul>\n<\/li>\n<li><strong>Cytometry Select (CS) <\/strong>covers speciality, advanced topics:\n<ul>\n<li>CS1: Cell Sorting<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>* FCE and CS1 courses are a pre-requisite for all new clients to use our <a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/flow-cytometry\/\">cell sorting<\/a> services. <\/strong><\/p>\n<p><strong>* FCE course is a pre-requisite for clients who want to take our Analyzer Training Course to operate our <a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/flow-cytometry\/\">analytical instruments<\/a> independently.<\/strong><\/p>\n<p>To access the courses, submit a <strong>FluoroFinder Academic Plus Membership Request<\/strong> (listed under the Training Services category) in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\" target=\"_blank\" rel=\"noopener noreferrer\">iLab<\/a>.<\/p>\n<\/div><\/div><\/div><div class=\"fusion-panel panel-default panel-129ece376f18b2a90 fusion-toggle-has-divider\" style=\"--awb-title-color:#64a0c8;--awb-content-color:#6b6b6b;\"><div class=\"panel-heading\"><h4 class=\"panel-title toggle\" id=\"toggle_129ece376f18b2a90\"><a aria-expanded=\"false\" aria-controls=\"129ece376f18b2a90\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-142-1\" data-target=\"#129ece376f18b2a90\" href=\"#129ece376f18b2a90\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">Analytical Cytometer Training<\/span><\/a><\/h4><\/div><div id=\"129ece376f18b2a90\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_129ece376f18b2a90\"><div class=\"panel-body toggle-content fusion-clearfix\">\n<p>Clients who complete our Analyzer Training Course will be endowed with the expertise to operate our <a href=\"https:\/\/lab.research.sickkids.ca\/fcf\/flow-cytometry\/\">analytical instruments<\/a> independently. In addition to teaching the basics of instrument start-up &amp; shutdown, this intensive course outlines current best practices for optimizing instrument set-up to ensure precision, accuracy and reproducibility in your data acquisition.<\/p>\n<ul>\n<li><em>To reflect our new suite of analytical instruments, the course is now known as Flow Cytometry Analyzer Training Course (formerly LSR Training Course)<\/em><\/li>\n<li><strong><em>This course is intended for clients who plan to independently operate our analyzers frequently (at least twice\/month)<\/em><\/strong><\/li>\n<li><em>An &#8220;intention to use our resources&#8221; is now codified in our training fee structure:<\/em>\n<ul>\n<li><em>in addition to the training fee ($500), a &#8220;pre-charge of $1,000 for future analyzer use (20-25 hours within one year from course completion) is now included<\/em><\/li>\n<li><em>before training will be scheduled, the fee must be approved by the trainee&#8217;s PI or financial manager <\/em><\/li>\n<li><em>following course completion, the cost of each analyzer experiment run by the trainee will be deducted from the $1,000 pre-charge<\/em><\/li>\n<\/ul>\n<\/li>\n<li><em>Clients who require less frequent or extensive analyzer use should instead request our &#8220;Assisted Acquisition&#8221; service<\/em><\/li>\n<\/ul>\n<p><strong>Pre-requisites:<\/strong><\/p>\n<ul>\n<li><em>FluoroFinder<\/em> Online Training Course:\n<ul>\n<li>Section 1: Principles &amp; Instrumentation<\/li>\n<li>Section\u00a02:\u00a0Reagents &amp; Panel Design<\/li>\n<li>Section\u00a03: Acquisition &amp; Measurements<\/li>\n<li>Section\u00a04: Analysis (with Interactive Tools)<\/li>\n<li>Section 5: Statistics<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>The Analyzer Training Course includes:<\/strong><\/p>\n<ul>\n<li>Two webinar lectures:\n<ul>\n<li><em><strong>Webinar 1:<\/strong> Optimizing &amp; Standardizing Instrument Set-up (2 h)<\/em><\/li>\n<li><em><strong>Webinar 2:<\/strong> Introduction to BD FACSDiva Acquisition Software (1.5 h)<\/em><\/li>\n<\/ul>\n<\/li>\n<li>Three hands-on instrument training sessions:\n<ul>\n<li><em><strong>Day 1:<\/strong> Demo of Instrument Set-up: 3 colour experiment using stained compensation beads (3 h)<\/em><\/li>\n<li><em><strong>Day 2:<\/strong> Trainee optimizes instrument settings under guidance from Facility Trainer (3 h)<\/em><\/li>\n<li><em><strong>Day 3:<\/strong> Trainee optimizes and standardizes Instrument set-up for their own experimental samples with the Facility Trainer (3 h)<\/em><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>The hands-on instrument training sessions cover the following topics:<\/p>\n<ul>\n<li>Overview of key instrument components (optics, electronics &amp; fluidics)<\/li>\n<li>Instrument start-up &amp; fluidics checks<\/li>\n<li>Introduction to acquisition software<\/li>\n<li>Optimization of instrument set-up (including flow &amp; event rates, PMT voltages, threshold, area scaling, compensation &amp; acquisition criteria)<\/li>\n<li>Data acquisition<\/li>\n<li>Cleaning &amp; shutdown procedures<\/li>\n<\/ul>\n<p>To request training, submit an <strong>Analyzer Training Course<\/strong> Request (listed under the Training Services category) in <a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\" target=\"_blank\" rel=\"noopener noreferrer\">iLab<\/a>.<\/p>\n<h2><\/h2>\n<\/div><\/div><\/div><div class=\"fusion-panel panel-default panel-07e104512ff2c3ba4 fusion-toggle-has-divider\"><div class=\"panel-heading\"><h4 class=\"panel-title toggle\" id=\"toggle_07e104512ff2c3ba4\"><a aria-expanded=\"false\" aria-controls=\"07e104512ff2c3ba4\" role=\"button\" data-toggle=\"collapse\" data-parent=\"#accordion-142-1\" data-target=\"#07e104512ff2c3ba4\" href=\"#07e104512ff2c3ba4\"><span class=\"fusion-toggle-icon-wrapper\" aria-hidden=\"true\"><i class=\"fa-fusion-box active-icon awb-icon-minus\" aria-hidden=\"true\"><\/i><i class=\"fa-fusion-box inactive-icon awb-icon-plus\" aria-hidden=\"true\"><\/i><\/span><span class=\"fusion-toggle-heading\">AutoMACS Training<\/span><\/a><\/h4><\/div><div id=\"07e104512ff2c3ba4\" class=\"panel-collapse collapse \" aria-labelledby=\"toggle_07e104512ff2c3ba4\"><div class=\"panel-body toggle-content fusion-clearfix\">The AutoMACS Pro is a user-friendly, bench-top, automated column-based magnetic cell separation instrument.\u00a0 There are 10 pre-loaded separation programs that allow positive or negative selection of labelled target cells based on subset frequency &amp; antigen expression, as well as desired yield and purity.<\/p>\n<p>Pre-requisites:<\/p>\n<ul>\n<li>It is recommended that you consult with FCF staff and\/or local Miltenyi Biotec reps for advice about reagents and separation programs in advance.<\/li>\n<\/ul>\n<p>The AutoMACS hands-on training course consists of one ~30 min training session with an FCF staff member and covers the following topics:<\/p>\n<ul>\n<li>Brief intro to magnetic cell separation principles<\/li>\n<li>Overview of instrument key components<\/li>\n<li>Instrument start-up &amp; fluidics checks<\/li>\n<li>Overview of software<\/li>\n<li>Separation program set-up<\/li>\n<li>Cleaning &amp; shutdown procedures<\/li>\n<\/ul>\n<p>To request training, submit an <strong>AutoMACS Training<\/strong>\u00a0Request (listed under the Training Services category) in\u00a0<a href=\"https:\/\/ca.ilab.agilent.com\/service_center\/show_external\/4715\/sickkidsuhn_flow_cytometry_facility\/\" target=\"_blank\" rel=\"noopener noreferrer\">iLab<\/a>.<\/div><\/div><\/div><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-142","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Instrument Training - SickKids-UHN Flow Cytometry Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/fcf\/instrument-training\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Instrument Training\" \/>\n<meta property=\"og:url\" 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