{"id":785,"date":"2017-11-09T11:41:21","date_gmt":"2017-11-09T11:41:21","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/fcf\/?post_type=avada_faq&p=785"},"modified":"2017-11-09T20:55:54","modified_gmt":"2017-11-09T20:55:54","slug":"no-events-in-acquisition-display-and-run-button-is-green","status":"publish","type":"avada_faq","link":"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/no-events-in-acquisition-display-and-run-button-is-green\/","title":{"rendered":"No events in acquisition display and RUN button is green"},"content":{"rendered":"
\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Observation<\/strong><\/th>Possible causes<\/strong><\/th>Recommended solutions<\/strong><\/th>\n<\/tr>\n<\/thead>\n
No events in acquisition display and RUN button is green<\/td>Threshold is not set to the correct parameter (usually FSC)<\/td>Set the threshold to the correct parameter for your application<\/td>\n<\/tr>\n
Threshold level is too high<\/td>Lower the threshold level<\/td>\n<\/tr>\n
PMT voltage for threshold parameter is set too low<\/td>Set the PMT voltage higher for the threshold parameter<\/td>\n<\/tr>\n
Gating issue<\/td>Ensure gating strategy is appropriate<\/td>\n<\/tr>\n
Air in the sheath filter<\/td>Purge the filter<\/td>\n<\/tr>\n
Sheath and air line not connected to the sheath tank<\/td>Ensure sheath and air line are connected to the sheath tank<\/td>\n<\/tr>\n
O-ring is not in position in the sheath tank<\/td>Reposition O-ring in sheath tank<\/td>\n<\/tr>\n
Cracked tube\/ Bal seal (tube to SIP connection) problem<\/td>Replace tube<\/td>\n<\/tr>\n
No sample in the tube<\/td>Add sample to the tube or install a new sample tube<\/td>\n<\/tr>\n
Sample is not mixed properly<\/td>Mix the sample to suspend cells<\/td>\n<\/tr>\n
Waste tank is full<\/td>Empty the waste tank<\/td>\n<\/tr>\n
PMT voltages set too low or too high for display parameter<\/td>Reset the PMT voltages<\/td>\n<\/tr>\n
Too few events are displayed<\/td>Increase the number of events to display<\/td>\n<\/tr>\n
Sample injection tube is clogged<\/td>Remove the sample tube to allow backflushing and prime the instrument several times. Clean instrument. Contact FMCF staff if issue is not resolved<\/td>\n<\/tr>\n
Laser is not warmed up<\/td>Wait the recommended amount of time for the laser to warm up.
\n- 30 min for the 488-nm (blue)
\n- 30 min for the 355-nm (UV)
\n- 15 min for the 405-nm (violet)
\n- 20 min for the 633-nm (red)<\/td>\n<\/tr>\n
Laser delay is set incorrectly<\/td>Contact FMCF staff<\/td>\n<\/tr>\n
Laser is not functioning<\/td>Verify the malfunction by changing the threshold to an alternative laser while running the appropriate sample. If unsuccessful, contact FMCF staff<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<\/div>
<\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[9],"class_list":["post-785","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-troubleshooting"],"yoast_head":"\nNo events in acquisition display and RUN button is green - SickKids-UHN Flow Cytometry Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/no-events-in-acquisition-display-and-run-button-is-green\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"No events in acquisition display and RUN button is green\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/no-events-in-acquisition-display-and-run-button-is-green\/\" \/>\n<meta property=\"og:site_name\" content=\"SickKids-UHN Flow Cytometry Facility\" \/>\n<meta property=\"article:modified_time\" content=\"2017-11-09T20:55:54+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/no-events-in-acquisition-display-and-run-button-is-green\/\",\"url\":\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/no-events-in-acquisition-display-and-run-button-is-green\/\",\"name\":\"No events in acquisition display and RUN button is green - 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