{"id":735,"date":"2017-11-09T14:00:52","date_gmt":"2017-11-09T14:00:52","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/fcf\/?post_type=avada_faq&p=735"},"modified":"2018-01-23T19:20:54","modified_gmt":"2018-01-23T19:20:54","slug":"how-many-cells-do-i-need-bring-to-the-sorter","status":"publish","type":"avada_faq","link":"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/how-many-cells-do-i-need-bring-to-the-sorter\/","title":{"rendered":"How many cells do I need to bring to the sorter?"},"content":{"rendered":"

This is the most commonly asked question. To answer it we need to know the following information:<\/p>\n

    \n
  1. What is the approximate percentage of the population(s) you wish to sort?<\/li>\n
  2. How many total cells do you want back?<\/li>\n
  3. Do you want stringent purification or enrichment?<\/li>\n
  4. How fragile or large are your cells?<\/li>\n<\/ol>\n

    All of these parameters influence the yield and purity of cell sorting. Here is an example: Let’s say the subset you want sorted is 20% of the total and you need 2 million cells for your experiment. In theory you would need to run 10 million cells through the sorter (10 million x 20% = 2 million). However, the actual yield is usually 75-95% of this theoretical yield, due to abort rates (caused by sort conflicts) and also the quality of your sample.<\/p>\n

    Therefore, we recommend that you bring 25-50% more cells to the sorter than you would need if the actual yield were 100% (based on the abundance of your target cells).<\/p>\n<\/div>

    <\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[7],"class_list":["post-735","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-cell-sorting"],"yoast_head":"\nHow many cells do I need to bring to the sorter? - SickKids-UHN Flow Cytometry Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/how-many-cells-do-i-need-bring-to-the-sorter\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How many cells do I need to bring to the sorter?\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/how-many-cells-do-i-need-bring-to-the-sorter\/\" \/>\n<meta property=\"og:site_name\" content=\"SickKids-UHN Flow Cytometry Facility\" \/>\n<meta property=\"article:modified_time\" content=\"2018-01-23T19:20:54+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/how-many-cells-do-i-need-bring-to-the-sorter\/\",\"url\":\"https:\/\/lab.research.sickkids.ca\/fcf\/faq-items\/how-many-cells-do-i-need-bring-to-the-sorter\/\",\"name\":\"How many cells do I need to bring to the sorter? 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