{"id":8,"date":"2015-10-08T23:57:30","date_gmt":"2015-10-08T23:57:30","guid":{"rendered":"https:\/\/lab.research.sickkids.ca\/campos\/?page_id=8"},"modified":"2018-08-28T16:30:42","modified_gmt":"2018-08-28T20:30:42","slug":"research","status":"publish","type":"page","link":"https:\/\/lab.research.sickkids.ca\/campos\/research\/","title":{"rendered":"Research"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-overflow:visible;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last\" style=\"--awb-bg-size:cover;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><script type=\"text\/javascript\">jQuery(function() { _initLayerSlider( '#layerslider_4_1bme36yp18dgf', {sliderVersion: '6.1.0', type: 'fixedsize', responsiveUnder: 1280, allowFullscreen: true, slideBGSize: 'auto', hideUnder: 0, hideOver: 100000, pauseOnHover: 'enabled', skin: 'noskin', sliderFadeInDuration: 350, globalBGColor: 'transparent', useSrcset: true, skinsPath: 'https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/plugins\/LayerSlider\/assets\/static\/layerslider\/skins\/', performanceMode: true}); });<\/script><link href=\"https:\/\/fonts.googleapis.com\/css?family=Open+Sans:100,100i,200,200i,300,300i,400,400i,500,500i,600,600i,700,700i,800,800i,900,900i%7CLato:100,100i,200,200i,300,300i,400,400i,500,500i,600,600i,700,700i,800,800i,900,900i%7CIndie+Flower:100,100i,200,200i,300,300i,400,400i,500,500i,600,600i,700,700i,800,800i,900,900i\" rel=\"stylesheet\"><div id=\"layerslider_4_1bme36yp18dgf\" data-ls-slug=\"Epigenetics_Slider\" class=\"ls-wp-container fitvidsignore ls-selectable\" style=\"width:1000px;height:500px;margin:0 auto;margin-bottom: 0px;\"><div class=\"ls-slide\" data-ls=\"duration:4000;transition2d:1;timeshift:-1000;\"><img decoding=\"async\" width=\"1086\" height=\"751\" src=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/Gel-2-blurry.jpg\" class=\"ls-l ls-img-layer\" alt=\"protein gel 1\" srcset=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/Gel-2-blurry-300x207.jpg 300w, https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/Gel-2-blurry-1024x708.jpg 1024w, https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/Gel-2-blurry.jpg 1086w\" sizes=\"(max-width: 1086px) 100vw, 1086px\" style=\"width:100%;top:-160px;left:-1px;white-space:normal;\" title=\"protein gel\" data-ls=\"offsetxin:80;offsetxout:-80;durationout:400;parallaxlevel:0;\"><img decoding=\"async\" width=\"1000\" height=\"466\" src=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/Graph-1-full2-e1448856453486.png\" class=\"ls-l ls-img-layer\" alt=\"replication fork\" style=\"top:6px;left:154px;white-space:normal;\" title=\"replication fork\" data-ls=\"offsetxin:80;delayin:1500;scalexin:0;scaleyin:0;offsetxout:-80;durationout:400;parallaxlevel:0;\"><p style=\"font-weight: 300;height:40px;padding-right:10px;padding-left:10px;font-family:Lato, 'Open Sans', sans-serif;font-size:30px;line-height:37px;color:#ffffff;background:#82d10c;border-radius:3px;top:361px;left:12px;\" class=\"ls-l ls-text-layer\" title=\"Inheritance of\" data-ls=\"durationin:2000;delayin:1500;easingin:easeOutElastic;rotatexin:-90;transformoriginin:50% top 0;offsetxout:-200;parallaxlevel:0;\">Inheritance of<\/p><p style=\"font-family:'Indie Flower';font-size:31px;color:#6db509;top:405px;left:36px;\" class=\"ls-l ls-text-layer\" title=\"Epigenetic Info\" data-ls=\"durationin:2000;delayin:2000;easingin:easeOutElastic;rotatexin:90;transformoriginin:50% top 0;offsetxout:-400;durationout:400;parallaxlevel:0;\">Epigenetic Information<\/p><\/div><div class=\"ls-slide\" data-ls=\"duration:4000;transition2d:1;timeshift:-1000;\"><img decoding=\"async\" width=\"2867\" height=\"2196\" src=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/10\/gel-1.jpg\" class=\"ls-l ls-img-layer\" alt=\"protein gel 2\" srcset=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/10\/gel-1-180x138.jpg 180w, https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/10\/gel-1-300x230.jpg 300w, https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/10\/gel-1-1024x784.jpg 1024w, https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/10\/gel-1.jpg 2867w\" sizes=\"(max-width: 2867px) 100vw, 2867px\" style=\"top:0px;left:50%;\" title=\"protein gel\" data-ls=\"offsetxin:600;durationin:2000;offsetxout:-600;durationout:400;parallaxlevel:0;\"><img decoding=\"async\" width=\"1000\" height=\"572\" src=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/trace-blurry1-e1448847193285.png\" class=\"ls-l ls-img-layer\" alt=\"chromatography\" style=\"top:-69px;left:210px;\" title=\"chromatography\" data-ls=\"offsetxin:900;durationin:2000;delayin:1000;offsetxout:-900;durationout:400;parallaxlevel:0;\"><p style=\"font-weight: 300;height:40px;padding-right:10px;padding-left:10px;font-family:Lato, 'Open Sans', sans-serif;font-size:30px;line-height:37px;color:#ffffff;background:#f04705;border-radius:3px;top:170px;left:174px;\" class=\"ls-l ls-text-layer\" title=\"BIOCHEMICAL\" data-ls=\"durationin:2000;delayin:1500;easingin:easeOutElastic;rotatexin:-90;transformoriginin:50% top 0;offsetxout:-200;parallaxlevel:0;\">HISTONES<\/p><p style=\"font-family:'Indie Flower';font-size:31px;color:#f04705;top:210px;left:183px;\" class=\"ls-l ls-text-layer\" title=\"Purification\" data-ls=\"durationin:2000;delayin:2000;easingin:easeOutElastic;rotatexin:90;transformoriginin:50% top 0;offsetxout:-400;durationout:400;parallaxlevel:0;\">&amp; histone chaperones<\/p><\/div><div class=\"ls-slide\" data-ls=\"duration:4000;transition2d:1;timeshift:-1000;\"><img decoding=\"async\" width=\"1200\" height=\"1041\" src=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/PPI-blurry.png\" class=\"ls-l ls-img-layer\" alt=\"protein protein interaction\" srcset=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/PPI-blurry-300x260.png 300w, https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/PPI-blurry-1024x888.png 1024w, https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/PPI-blurry.png 1200w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" style=\"top:-70px;left:453px;\" title=\"protein protein interactions\" data-ls=\"offsetxin:400;durationin:2000;offsetxout:400;durationout:400;parallaxlevel:0;\"><img decoding=\"async\" width=\"500\" height=\"768\" src=\"https:\/\/lab.research.sickkids.ca\/campos\/wp-content\/uploads\/sites\/15\/2015\/11\/FPLC-edited-e1448854037472.png\" class=\"ls-l ls-img-layer\" alt=\"FPLC\" style=\"top:-94px;left:-22px;\" title=\"FPLC\" data-ls=\"offsetxin:-200;durationin:2000;offsetxout:-200;durationout:400;parallaxlevel:0;\"><p style=\"font-weight: 300;height:40px;padding-right:10px;padding-left:10px;font-family:Lato, 'Open Sans', sans-serif;font-size:30px;line-height:37px;color:#ffffff;background:#544f8c;border-radius:3px;top:91px;left:547px;\" class=\"ls-l ls-text-layer\" title=\"CHROMATIN\" data-ls=\"durationin:2000;delayin:1500;easingin:easeOutElastic;rotatexin:-90;transformoriginin:50% top 0;offsetxout:-400;parallaxlevel:0;\">CHROMATIN<\/p><p style=\"font-family:'Indie Flower';font-size:31px;color:#544f8c;top:137px;left:613px;\" class=\"ls-l ls-text-layer\" title=\"Biology\" data-ls=\"durationin:2000;delayin:2000;easingin:easeOutElastic;rotatexin:90;transformoriginin:50% top 0;offsetxout:-600;durationout:400;parallaxlevel:0;\">Biology<\/p><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last\" style=\"--awb-bg-size:cover;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-column-content-centered\"><div class=\"fusion-column-content\"><div class=\"fusion-text fusion-text-1\"><h2>My laboratory centers on the topic of chromatin biology in health and disease, with a particular focus on histone proteins.\u00a0Histones play a critical structural role by organizing and packaging DNA. Our genome encodes numerous histone variants, all of which are subject to a large number of posttranslational modifications. These variants, and modifications, help dictate proteins that bind local chromatin structures and therefore carry tremendous influence on the expression of underlying genes. This information can be transmitted through numerous cell cycles, and is believed to be epigenetic.<\/h2>\n<h2><\/h2>\n<h2>Two main themes have been developed in my lab, namely: i) the study of proteins that influence the inheritance of epigenetic information; and ii) the study of histone-binding proteins whose deregulation is pathogenic.<\/h2>\n<h2><\/h2>\n<h2>i) Inheritance of \u00a0epigenetic information: While histones carry important information, they are also routinely displaced and modified as DNA is replicated, transcribed and repaired. We aim to understand the spatiotemporal regulation of epigenetic factors that cells utilize to maintain a transcriptional \u2018memory\u2019 through cell division.<\/h2>\n<h2><\/h2>\n<h2>ii) Histone binding proteins: Histones are chaperoned, moved, and bound by a myriad of proteins. In our studies, a particular emphasis is placed on the biochemical characterization of histones, histone chaperones, as well as other histone-binding proteins, and how their functions go awry in a number of childhood disorders.<\/h2>\n<\/div><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-2 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last fusion-column-no-min-height\" style=\"--awb-bg-size:cover;--awb-margin-bottom:0px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-2\"><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":54,"featured_media":0,"parent":0,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-8","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - 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