The Agilent Seahorse XF Cell Mito Stress Test measures key parameters of mitochondrial function (basal respiration, ATP-linked respiration, maximal respiration, spare capacity, proton leak, non-mitochondrial oxygen consumption) by directly measuring the oxygen consumption rate (OCR) of cells.
The Agilent Seahorse XF Real-Time ATP Rate Assay measures total ATP production rates in living cells and is able to distinguish between the fractions of ATP that are produced from mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, the two main metabolic pathways responsible for ATP production in mammalian cells.
The Agilent Seahorse XF Glycolytic Rate Assay is an accurate and reliable analytical method for measuring glycolysis in cells. The assay utilizes both extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) measurements to determine the glycolytic proton efflux rate (glycoPER) of the cells.